SU1708828A1 - Method of separation of chorioallantois membranes from chick embryos - Google Patents

Abstract

The invention relates to the microbiological industry, in particular to the production of chicken embryo (CE) vaccine preparations, and can be used in the development of automated equipment. The purpose of the invention is to reduce the labor intensity and increase the productivity of the method. To do this, in the proposed method, which includes opening the shell, breaking the HAO bond with the internal structures of the embryo, removing the inner structures and selecting the chorioallantoic membranes (HAO) from the shell, the CE is oriented vertically with the sharp end of the shell down, a hole in the shell is punched from the blunt end shell and chorioallantoic membranes, the shell opening is performed by cutting from the sharp end to a diameter of 34-36 mm with simultaneous destruction of the HAO bond with the internal structures of the embryo, after which the internal structures are removed from the shell by gravity and HAO is sampled by creating inside the shell a flow of air with a flow-directed air of at least 1.4 to 10 m / s. 2 tab., 1 sludge.

Description

The invention relates to the microbiological industry, in particular to the production of veterinary vaccines based on chicken embryos (CE), and can be used in the development of automated equipment.

There is a method of manual selection of chorioallantoic shells (HAO), in which CEs are placed horizontally. The opening of the shell is conducted from the side of the side surface in the region of the artificially formed air chamber. Then, HAC is incised. The CEs are turned with a hole downwards and internal structures (C C) are removed from it, mechanically destroying their connection with HAO. After that, HAO is taken from the shell cavity using tweezers.

The disadvantage of this method is the high complexity of the method.

The purpose of the invention is to reduce the labor intensity and increase the productivity of the selection method.

The drawing is a diagram explaining the proposed method.

When selecting HAO, CEs are oriented vertically with a sharp end down. From the side of the blunt end, punch a hole (a) in the shell, underwater, and choriolantoic shells. Open the shell, cut it from the side of the sharp end of the diameter of 34-36 mm (b). At the opening, the connection between the internal structures of CE and HAO is destroyed. The internal structures are removed by gravity from the shell through the open hole, and the hole at the blunt end serves to allow air to enter the shell cavity. After removal of the internal structures, the remaining HAO inside the shell is selected by creating a directional airflow (c) in the shell cavity. The use of the method makes it possible to reduce the laboriousness of the operation due to its mechanization and to reduce its time. The proposed method is verified experimentally. In the course of the experiments, the following parameters were determined: the minimum diameter of the opening of the CE shell, which guaranteedly destroys the connection of the HAO with the internal structures of the embryo; the amount of air flow is sufficient for the collection of HAO from the shell (see tab. 1 and 2). Experiments have shown that the minimum diameter of the opening of the shell is 3436 mm, and the air flow rate is not less than 1.4-10 m / s. The total time of the operation when opening the FE at a diameter of 3436 mm and an air flow rate of 1.5-10 m / s 3 average is 5 s. The average time for the dependence of the destruction of the link HAO with the internal structures of the diameter of the opening

Table 1

Claims (1)

The shells spent on the selection operation are 36 seconds. Formula of the invention A method for selecting chorioallantoic membranes from chick embryos, including opening the shell, breaking the connection of the chorioallantoic shell with the internal structures of the embryo, removing the internal structures and selecting the chorioallantoic shell from the shell, characterized in that. In order to reduce the labor intensity and increase the productivity of the method, before inserting the shells, the chicken embryo is oriented with the vertical end of the shell with the sharp end down, and from the blunt end they pierce the hole in the shell, the hypochondral and chorioallantoic membranes, the dissection is carried out simultaneously with the destruction of the lining of the chorioallastic disperse bed pulp. from the side of the sharp end to create a hole with a diameter of 34-36 mm, the internal structures are removed by gravity, and the selection of the chorioallantoic membrane is about uschestvl removed by providing inside the shell directed airflow with flow rate namenya 1,4-10. Continued table. .one Dependence of HAO selection on air consumption Table 2 Freedom