SU1708400A1 - Method of producing sorbent for hemosorption - Google Patents

Abstract

The invention relates to the preparation of sorbents with polymer coatings and allows to increase the efficiency of hemosorption. The thickness of the fibrin film V covering 1 cm of coal can be varied by varying the concentration of thrombin 1–3 mg / ml, fibrinogen 35–45 mg / ml without the formation of conglomerates with the following sequence of introduction of the components during the formation of the fibrin film: carbon granules prepared to the coating, centrifuged until the phases of the solution are completely removed, treated with a solution of thrombin with a concentration of 1-3 mg / ml for 20-30 minutes, remove the excess of thrombin by washing with saline, re centrifuging, then treating with fibrinogen with a concentration of 35-45 mg / ml for 5-10 min t / t, the resulting sorbent with a fibrin film is washed from excess fibrinogen with saline. 2 tablets

Description

The invention relates to the field of the preparation of sorbents with polymer coatings and can be used in medical practice, namely in the purification of blood from toxins.

The aim of the invention is to increase the efficiency of the process of hemosorption by preventing the formation of a conglomerate of sorbent granules.

Preventing the formation of coal granule conglomerates is achieved by treating the granules with a solution of thrombin with a concentration of 1-3 mg / ml. removing unabsorbed thrombin, followed by treating the granules with a solution of fibrinogen with a concentration of 35-45 mg / ml, followed by removal of the residue of unreacted thrombin.

PRI me R. As a starting sorbent for coating the fibrin film, take the floconic hemosorbent SKN-1K large-pore. The size of spherical granules is 0.5-1 mm. The strength of hemosorbent is at least 80%, the volume of sorption pores (for benzene) is at least 0.9 cm / g.

The coal bottle is uncorked and the hemosorbent is removed from the saline solution, the granules are transferred into a plastic tube with a perforated bottom to a mark corresponding to a volume of 1 cm, the saline is drained and the granules with a plastic tube are more centrifuged for more complete removal of the physiological residues for 1 min. solution. This residual volume of saline is measured and is 0.4 ml. To coal prepared in this way

0.5 ml of the thrombin solution of the corresponding concentration (ie, the volume slightly exceeds 0.4 ml) is poured into the granules. The thrombin solution is incubated with angular granules for 20-30 minutes. During this time, the physical sorption of thrombin is almost completely carried out in the pores of the granules. Then, the coal granules are washed from un-sorbed thrombin by fifteen times compared to the volume of coal with a volume of saline. The granules are re-centrifuged for 1 minute to remove residual saline in order not to dilute the fibrinogen concentration in the process of fibrinogen sorption following the centrifugation.

The initial concentration of the fibrinogen solution prior to sorption is prepared by dissolving a portion of fibrinogen in an appropriate volume of saline. In this case, the volume of the fibrinogen solution is 0.4 ml for every 1 cm of SKN 1K coal, i.e. minimum volume required only for wetting the pores of the coal granules.

Coal granules are kept in the fibrinogen solution for 5-10 minutes. During this time, depending on the concentration of fibrinogen, 65-90% of fibrinogen is sorbed and simultaneously polymerized. The resulting hemosorbent coated with fibrin film is washed again with a volume of fifteen times as compared with the volume of carbon of the saline solution into which non-sorbed fibrinogen passes. The volume of washing solutions taken into account. Washing is carried out to the complete absence of traces of non-sorbed protein in the washing solutions. The concentration of non-sorbed fibrinogen is determined by measuring the optical density of the washing solutions at a wavelength of 280 nm using an SF-26 spectrophotometer. Using the dependence of optical density on the concentration of fibrinogen (calibration graph), the concentration of non-adsorbed fibrinogen is found. Knowing the volume of washing solutions and the concentration of fibrinogen, the amount of fibrinogen in the discharge after sorption is determined. The difference in the amount of fibrinogen at the entrance to coal (0.4 ml) and in the discharge determines the magnitude of fibrinogen sorption.

The results of studies of the influence of concentrations of solutions of thrombin and fibrinogen, as well as the sequence of sorption

thrombin (T) - fibrinogen (f) or ft are summarized in table 1.

From table 1 it follows that the sorbent obtained by processing thrombin

(1-3 mg / ml) - fibrinogen (35-45 mg / ml), are optimal for hemosorption fibrin film thickness, preventing adhesion of the granules.

Improving the efficiency of hemosorption. Sorbents SKN-1K of commercial production of SKN-1K, coated with fibrin film according to F-T scheme from 35 and 45 mg / ml fibrinogen solutions, as well as coated with fibrin film according to T-F from 35 and 45 mg / ml fibrinogen solutions and thrombin concentration 2 mg / ml, tested in laboratory conditions for the sorption of medium molecules using the marker of medium molecules Pi2 (M.v. 1350),

To do this, 3 ml of vitamin Bi2, containing 600 µg of vitamin, are incubated for 1 hour with 1 cm of the indicated sorbents and shaken periodically. After incubation, the supernatant is removed from the vial.

liquid and spectrophotometrically measured to determine the optical density of unabsorbed vitamin Bi2 on an SF-26 spectrophotometer at a wavelength of 361 nm. Using the calibration graph of the dependence of the optical G1 density of a standard solution of vitamin B12 on its concentration, the content of non-adsorbed vitamin Bi2 is determined. According to the difference in vitamin content before incubation with sorbents and after

find the amount of sorption. The test results are shown in table 2.

From the data of table 2 it can be seen that sorbtsi. vitamin Bi2 on the sorbent, obtained by the scheme TF, 3-9% more than

coal covered with fibrin film according to the ft scheme.

Claims (1)

Claims A method for producing a sorbent for hemosorption, comprising treating coal granules with thrombin and fibrinogen solutions, characterized in that, in order to increase the efficiency of the hemosorption process by preventing the formation of conglomerates of sorbent granules, coal granules are treated with a solution of thrombin with a concentration of 1-3 mg / ml then i-non-absorber & thrombin thrombin is removed and treated with fibrinogen solution with concentration of 35-45 mg / ml, followed by removal of the residue of unreacted fibrinogen. t a b ji and k "1 T a 6 L and c a 2