SU1703679A1 - @@@ incubator - Google Patents

Abstract

The invention relates to a biological technique, namely to CO2 incubators designed to preserve and cultivate bioobjects. The purpose of the invention is to maintain stable conditions of the environment in an incubator. The incubator contains a thermostatically controlled chamber 1 with a door installed in the chamber 1 of the shelf 3, cells 4 with biological objects placed in them, vertical partitions 6 with the formation of compartments 7 for cells 4, while the partitions 6 and the side walls 8 of the cells 4 have holes 9 and 10 for the passage of gas, located opposite each other. In addition, the incubator contains heating elements 11. a fan 12 with a drive 13, a gas supply system 14 and a sump 15 with water. The proposed CC 2 incubator provides stable conditions for the cultivation environment and allows them to be maintained during loading and unloading of biological objects. 3 sludge. Ё 1 10 VJ About with about VI VI 15

Description

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The invention relates to a biological technique, namely to a biological C02 incubator, intended for storage and cultivation in a specific gas environment with a certain temperature and humidity of various bioobjects. for example cell cultures.

The purpose of the invention is to maintain stable environmental conditions in the incubator.

1 shows a CO2 incubator, a longitudinal section; figure 2 - section aa in figure 1; on fig.Z - retractable cell, axonometric.

The C02 incubator contains a thermostatically controlled chamber 1 with a door 2, placed in the chamber 1 at a height horizontal shelves 3 with retractable cells 4 for accommodating bioobjects 5. mounted on horizontal shelves 3, vertical partitions 6 with the formation of compartments 7 for retractable cells 4 , while the partitions 6 and the side walls 8 of the cells 4 have openings 9 and 10 for the passage of gas opposite each other. In addition, the incubator contains heating elements 11, a circulation fan 12 with a drive 13, a gas mixture supply system 14, and a water pan 15 with water located at the bottom of the chamber to humidify the atmosphere in the incubator.

C02 incubator works as follows.

When the incubator is turned on, the fan 12 creates a forced convection of the atmosphere inside the thermally insulated chamber 1 with a closed circulation circuit. The gas medium moves evenly in the working volume of chamber 1 (Fig. 1, arrows), successively passing all compartments 7 through the corresponding through holes 9 and 10. The control and regulation unit using a temperature sensor and CO2 detection detector (not shown) installed in chamber 1 controls the operation of the heating elements 11 and the gas mixture supply system 14. Due to the natural evaporation of the water in the heated tray 15, the circulating gaseous medium of the incubator is saturated with moisture. When the sump 15 is filled with distilled water, the incubator chamber will provide the maximum level of relative humidity (95-96%) for all operating temperatures.

Upon reaching the established values of a predetermined temperature and gas environment parameters, the incubator is ready for operation.

For placing bioobjects 5, the incubator door 2 is opened and the necessary cell 4 is pushed out of the corresponding compartment 7. At the same time, the stabilization of the temperature and gas phase in the remaining compartments of the incubator chamber is not disturbed, because when the incubation cell 4 is extended, the holes 10 in the vertical partitions 6 of the corresponding compartment 7 overlap the walls 8 of the extended cell 4. In the final extended position, the side walls 8 of the incubation cell 4 do not interfere with the passage of the gaseous medium through the compartment 7, and the rear wall Cells 4 prevent leakage of the internal atmosphere to the outside.

5 After installing the incubation cell back to the compartment 7, the holes 9 and 10 will be located opposite each other, due to which the circulation of the gas section through the installed cell 4 with the placed biological objects 5 is resumed.

Thus, in the proposed incubator, during loading and unloading, the established composition of the gaseous medium is stably maintained, the relative humidity and the given temperature conditions are high. necessary to maintain the vitality of cultivated biological objects.

The use of the proposed isotope in medical practice and in laboratory biological and microbiological studies with the content of cell and tissue cultures, as well as microorganisms, allows

5 in comparison with the prototype, to improve the conditions for their cultivation, and by preventing leakage of the gaseous medium during manipulations with biological objects, to increase the economy and reliability of this process.

0 In addition, the reduction of possible fluctuations of the controlled parameters of the gas environment during the laying and extraction of biological material increases the possibility of using the proposed

5 Soz incubator to maintain the viability of such sensitive bio-objects as reproductive cells of humans and animals at the stage of their maturation and development.

0

Claims (1)

Claims of the COA-incubator. containing a thermostatically controlled chamber with a door, horizontal shelves with 5 retractable cells for placing bioobjects therein, characterized in that. that, in order to maintain a stable environment in the incubator, vertical partitions are installed on horizontal shelves with the formation of compartments for retractable cells, while the partitions and side walls of the cells have openings for 1A-A 2 gas passage, located opposite each other. eight / / / 8 Rig.Z