SU1696434A1 - Method for preparation of steroid glycosides of furostane series - Google Patents

Abstract

This invention relates to the production of steroid glycosides in a row of furostan. The goal is to simplify the process and increase the growth of the stimulating effect. Production is carried out by extraction of potato seeds (Solanum tuberosum) with 70% ethanol. The extract is treated with chloroform, the aqueous layer is exhaustively treated with butanol-1, the butanol extracts are concentrated in vacuo, the residue is passed on a Sephadex column, the fractions containing glycosides are combined and evaporated to a dry residue. 1 tab.

Description

This invention relates to bioorganic chemistry, in particular to the preparation of new biologically active substances, steroidal glycosides of the furrostan variety from vegetable raw materials.

A known method for producing steroid glycosides from inflorescences and onion seeds consists in degreasing vegetable raw materials and extracting with methanol, concentrating the methanol extract, diluting the residue with water and extracting with butyl alcohol, and evaporating the resulting extract to a dry residue, which is precipitated acetone 1.

The disadvantage of this method is the use of acetone and methanol, which have a detrimental effect on human health. In addition, this method is rather laborious.

There is also known a method for producing capsicosides with a growth-promoting effect, which consists in the fact that

extraction of the roots of chilli peppers with methanol 2.

 The purpose of the invention is to simplify the process of obtaining steroid glycosides and increase the growth-promoting activity. The goal is achieved by using potato seeds (Solanum tuberosum L) as a source, extraction is carried out with 70% ethanol, the resulting extract is concentrated, treated with chloroform, then the aqueous layer is extracted with butanol-1, butanol extract is concentrated in vacuo to dry residue with further percolation on sexdex.

Example. 300 g of dried potato seeds (Solanum tuberosum L) are crushed, poured onto 1.5 l of 70% ethanol and extracted for 6 hours. Extraction is repeated four times. The extracts are combined, evaporated. The remaining sod extract is treated with chloroform

WITH

with

oh oh

B gj 4

(three times 300 ml each). The aqueous extracts are treated with butanol-1 (400 ml four times). The butanol extracts are concentrated in vacuo to a dry residue. The isolated target product is applied to a Sephadex column (h column 50 cm, d 2 cm) and washed with water. Next, one stripped off and the dried product is controlled in a thin layer of silica gel in the chloroform-methanol-water solvent system (65: 35: 7 - volume ratios), showing the chromatogram with Sagnier and Erlich reagents.

The fractions containing glycosides are combined and evaporated. The result is 10 g of the amount of glycosides (3.3% of the mass of the feedstock).

The sum of steroid glycosides from potato seeds is a white powder, easily soluble in water, ethyl alcohol, methyl alcohol, pyridine, insoluble in acetone, chloroform, diethyl ether. After total acid hydrate, the amount of aglycone is determined by physicochemical methods of mohenin, in the oligosaccharide part - galactose, glucose and ramnase. Chromatography on a thin layer of silica gel shows four pink spots of Ehrlich reagent with Rf 0.42; 0.36; 0.32: 0.29 (solvent system chloroform-methanol-water 65: 35: 7 v / v / v), which is typical for steroid glycosides and furostan.

To determine the biological activity of the preparation, aqueous solutions are prepared at a concentration of 0.01; 0.001; 0.0001.

The pre-calibrated Rainbow Tomato seeds are soaked in

24 h in test compound solutions. After the specified period, the seeds are removed, washed and laid out in Petri dishes. In each variant, 100 seeds are used. The repetition of the experiment is 3-fold in each version. On the 6th day, germination energy is taken into account, and on the 10th - germination. The control is distilled water.

At the same time, a structural analogue, capsicoside, is tested in the same concentrations. The base is a well-known drug - ivin.

The test results — the effect of the amount of steroid glycosides on the germination energy and germination of Rainbow seeds — are presented in the table.

From the data in the table of the video, that the resulting drug has a higher stimulating effect compared with the known drug, capsicoside.

Claims (1)

Claims of the method of producing steroid glycosides of the Furostan series, which have a growth-stimulating effect, by extracting vegetable raw materials, characterized in that, in order to simplify the process and increase the growth-promoting effect, potato seeds (Solanum tuberosum) are extracted with 70% ethanol, the extract treated with chloroform, the aqueous layer exhaustively treated with butanol-1, the butanol extracts are concentrated, vacuum, the residue is passed on a Sephadex column, the fractions containing glycosides are combined and evaporated to dryness.