SU1679371A1 - Device for measuring concentrations of multicomponents sugar solutions - Google Patents

Abstract

The invention relates to a measurement technique, in particular to devices for the optical analysis of multicomponent solutions, and can be used to automatically determine the concentration of substances dissolved in a liquid. The purpose of the invention is to improve the accuracy and expansion of the measurement range. The radiation source 1 (tunable infrared laser) is tuned to the wavelength characteristic of the selected wavelength. 1 Controllable Geshtrator CO recording unit Os XI S X X VM

Description

1

functional group of the test substance. The beam oj of the radiation source is directed to a double-beam interferometer, where it passes through the measuring channel in which the first chamber 2 of the cuvette is located, which is under the influence of a magnetic field created by the magnetic source, and through the control channel in which the second chamber of the cuvette 3 is located, affected by the magnetic field. On the output prism 12 of the interferometer, an interference pattern is observed, recorded by the radiation detector 8 and displayed on the display elements of the recording unit 15. The controlled oscillator b generates an alternating current in the radio frequency circuit 7 with the frequency necessary to produce nuclear proton magnetic resonance of the chosen functional group. In this case, a change in the phase, s of the beam of the measuring channel will entail a shift in the interference pattern, which will be displayed on the display elements of the registration unit 15.3 ill.

The invention relates to a measurement technique, in particular to devices for the optical analysis of multicomponent solutions, and can be used to automatically determine the concentration of substances dissolved in a liquid.

The aim of the invention is to improve the accuracy and the expansion of the measurement range.

The essence of the invention consists in the simultaneous use of instrumental means used in measurements by the method of infrared (IR) spectroscopy and nuclear magnetic resonance (NMR).

A comparison of the IR spectra and the NMR spectrum of the test substance and the rest of the mixture components makes it possible to isolate the signal about the amount of the test substance in a multicomponent solution. The use of the NMR effect allows introducing a certain proton of the functional group of the substance under investigation into the state of nuclear magnetic resonance. With simultaneous exposure to infrared radiation characteristic of the functional group under study, the phase change of infrared radiation occurs.

FIG. 1 shows a diagram of the device; in fig. 2 - IR spectrum / J-D - (+) glucose; in fig. 3 - proton magnetic resonance spectrum of / -D - (+) glucose.

The device (Fig. 1) contains a radiation source 1, made in the form of a tunable infrared laser, a cuvette in the form of two communicating (not shown) cameras 2 and 3 between them, the source of a magnetic field, between poles 4 and 5 of which is located first (measuring a cell chamber 2, a controlled generator 6, the output of which is connected to the radio frequency circuit 7, and a radiation detector 8. The chamber 2 of the cuvette is located inside the radio frequency circuit 7 and is placed in the measuring channel 9 of the Mach-Zander two-beam interferometer, and the chamber 3 is placed in the reference

channel 10 of the interferometer. The two-beam interferometer includes beam-splitting prisms 11 and 12 and reflectors 13 and 14. The output of the radiation detector 8 is connected to block 15.

registration. The operation of the device is controlled by the control panel 16, the outputs of which are connected to the radiation source 1 and controlled by the generator 6,

A laser manufactured according to the technology of producing multicomponent solid solutions and heterostructures of semiconductor compounds was used as a source of infrared radiation. As

The radiation decoder can be applied any known method and device for determining the shift of interference patterns.

The control panel contains available

the operator controls the tuning of the frequency of the controlled oscillator and the wavelength of the radiation source. As a controlled oscillator, radio frequency circuit, magnetic source can be

the corresponding modules are used, which are part of the commercially available nuclear magnetic resolution spectrometer type RYa-2305.

Let there be a solution consisting of

components: A, B, C, D, E, F, glucose.

The IR spectrum includes components A, B, C and.

the spectrum of glucose, and the NMR spectrum includes

components D, E, F and NMR spectrum of glucose.

Then, using IR spectroscopy, you can determine the total contribution of glucose and the components A, B, C. Similarly, using NMR spectroscopy will give the opportunity to determine the total contribution of glucose and the components D, E, F.

The infrared spectrum of a multicomponent solution (Fig. 2) contains zones of 1-valent OH oscillations, 11-valent oscillations of the C.N.SH-lattice change, IV-valent oscillations of CO.

The NMR spectrum of the same solution (Fig. 3)

contains the resonant frequencies of the protons I

- On, II - OH, III - On. IV - Hx, Nd, V - CHt.VI

- residual solvent signal. The spectrum was obtained with a magnetic field strength of 14 kG. 60 MHz operating frequency and 600 Hz sweep rate.5

From these spectra, for a specific functional group, for example, the C-H group that is part of glucose, the characteristic absorption wavelength in the IR spectrum is determined. For glucose, it is 3.43 microns. 10 Similarly, the NMR spectrum determines the value of the frequency of the alternating current induced in the radio frequency circuit 7 set by the controlled generator 6 for the occurrence of the C-15 N functional group NMR flux at magnetic field strength

14kGs generated by a magnetic source.

Using the data obtained from the IR and NMR spectra of glucose, the operator from the console adjusts the radiation source — the tunable laser of the AND K-band — to radiation with a wavelength of 3.43 µm. In this case, the controlled oscillator b is closed and in the RF circuit 7 the RF field is not induced. -25

The beam from the radiation source passes through the cell 2 of the cuvette under the influence of the magnetic field created by the magnetic source. At the same time, the beam of the control channel passes through 30 the chamber 3 of the cuvette, which is not affected by the magnetic field, and is directed to the prism 12 of the interferometer, on which the interference pattern is observed.

The operator places the radiation detector 8 at the center of the dark or light band of the interference pattern. The accuracy of the installation is determined by the size of the bl

15 registrations. Then the operator of the control panel 16 opens the controlled generation of 40

2.5

Length of 8 waves, micron 3.0 W 5.06.0 7.0 8.03 110 11 W 16

 80 60

§ y

20 § WOO 3500 3000 2500 2000 7800 7600 40Q 12QQ 1QQQ 800 625

Frequency, -1 Z

the torus 6. A controlled oscillator in the RF circuit 7 generates an alternating current with a frequency necessary for the appearance of the NMR proton of the C-H functional group. This changes the phase of the first beam in the measuring channel 9, which will cause a shift in the interference pattern. The amount of displacement is proportional to the number of C-H functional groups whose protons are in the NMR state, therefore, the amount of displacement is proportional to the amount of glucose in this solution.

Claims (1)

The invention The device dp measuring the concentration of multicomponent sugar solutions containing a cuvette, a radiation source, a magnetic field source, a radiation detector, characterized in that, in order to improve accuracy and extend the measurement range, it additionally contains a controlled radio frequency oscillator with a radio frequency circuit - a Mach – Zander interferometer with measuring and control channels, a control panel, the radiation source being a tunable laser infrared range, the cuvette is made in the form of two communicating cameras, with the first camera located inside the RF circuit between the poles of the magnetic field source and placed in the measuring channel and the second camera in the control channel of the double-beam interferometer, the two-beam interferometer located between the radiation source and the radiation detector a control panel is associated with a radiation source and a controlled radio frequency oscillator.