SU1677060A1 - Method of preparing nutrient antibiotics - Google Patents

Abstract

The invention relates to the production of antibiotics and can be used in the preparation of feed preparations. The purpose of the invention is to simplify the method and reduce the loss of antibiotic. The method includes the cultivation of the producer in a liquid nutrient medium, followed by separation of the culture medium and the introduction of the filler in the amount of 3-67 May. % After that, the medium is dried in a spray dryer. 1 z p f-ly

Description

The invention relates to the production of antibiotics and can be used in the preparation of feed preparations.

The aim of the invention is to simplify the method and reduce the loss of antibiotic.

The simplification of the method of obtaining an antibiotic is due to the fact that the addition of a filler to the culture medium makes it possible to exclude two operations — evaporation and standardization. As a result of the introduction of the filler, the solids content in the culture medium is increased in such a way that it is not necessary to thicken them by evaporation.

The creation of a low-waste technology is a consequence of the fact that, with the exception of the evaporation operation, the condensate is not included in the composition of wastewater. The method also makes it possible to dispose of fractions of the culture medium with a low content of antibiotic and, thus, not direct them to the treatment plant. Activated sludge from sewage treatment plants is used to increase the concentration of solids in the culture medium or its fractions, to increase the nutritional value and standardize products.

The loss of the antibiotic is reduced due to its lower inactivation, since during the dewatering process, this thermolabile substance is exposed to heat only once (during drying) and the addition of a filler slows down the destruction of the target product. In addition, there is no entrainment of the antibiotic into the sewage with condensate and recycled fractions of the culture medium.

The cost of producing the antibiotic is reduced due to the fact that its yield increases, the pollution becomes less and the cost of wastewater treatment and the need for equipment for vacuum evaporation and standardization is eliminated.

The proposed method is carried out as follows.

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As a producer, bacteria, fungi or other microorganisms synthesizing antibiotics are cultivated in liquid nutrient medium. The composition, pH and aeration level of the nutrient medium, temperature and duration of cultivation are determined based on the characteristics of the producer and the conditions necessary for maximum formation of the target product. After completion of biosynthesis, if necessary, reagents that inhibit the inactivation of the antibiotic are added to the culture medium, and the latter is concentrated and isolated as enriched fractions to obtain pure preparations or concentrates of the target product. The separation of the culture medium into fractions is carried out by filtration, centrifugation, sorption on ion exchange resins or other known methods. The filler is products of plant, microbial and animal origin, or a mixture of bran, biomass of microorganisms, skim milk, etc. It is highly advisable to use activated sludge microorganisms as biomass, especially in the form of a thickened suspension, which helps to solve the problem of utilization of wastewater biological purification products. These fillers are introduced into the culture medium or its fractions in an amount of 3-67% of their mass so that the resulting suspensions are suitable for direct drying and the resulting product contains a certain amount of antibiotic. In this case, the culture medium is understood to mean a product obtained by cultivating microorganisms on a nutrient medium, and the fractions of a culture medium are its parts in the form of suspensions and solutions, including the process flow.

PRI me R 1. Producer antibiotic grisin - Actinomyces griseus, strain 70, cultured at a temperature of 27 ± 1 ° C, pH 6.5-6.9 and aeration 32 g 02 per 1 m3 of medium per minute for 48 h on next Wednesday, May. %: corn flour 2,3; potato starch 1,4; ammonium sulfate 0.6; potassium phosphate disubstituted 0.02; magnesium sulfate 0.05; salt 0.2; chalk 0.5; enantic acid 0.1; propinol B-400 0.2; tap water else, The resulting culture medium with grizin is acidified with 30% hydrochloric acid to pH 5.5 and divided into two equal flows.

Finely ground wheat bran is added to the first stream in an amount of 3% of

mass of culture medium. The resulting composition is mixed and dried in a spray dryer at a heat carrier temperature of 135 ° C at the inlet and 55 ° C at

output As a result, 57.5 kg of kormogrizin 40, a standard antibiotic preparation containing 40 g of grisin per 1 kg of product, is obtained from 1 m3 of culture medium. The second stream (control) is evaporated in a vacuum evaporator at 60 ° C for 1 h, dried in a spray dryer under the above regime and the product obtained by dehydrating 1 m3 of culture medium, after grinding in

5 hammer crusher mixed with 21.7 kg of the same bran finely ground. As a result, 48.9 kg of kormogrizin 40 are obtained from 1 m of culture medium. At the same time, from 1 m3 of culture medium, 0.68 m of condensate with a COD of 57 ml / l 02 is fed into the wastewater.

PRI mme R 2. Producer antibiotic bacitracin - Bacillus licheniformis, strain 1001, cultured at a temperature of 37 ± 1 °

5 C, pH 7.0-7.2 and aeration 34 g 02 per 1 m3 of medium per minute for 30 hours on the medium of the following composition, May. %: Soybean flour 7.5; potato starch 2.0; calcium carbonate 1.0; magnesium sulfate 0.33; propynol B-400

0 0.2; water plumbing rest. The obtained culture medium with bacitraction is acidified with 30% hydrochloric acid to pH 6.0, stirred and divided into three streams.

5 To the first stream, yeast biomass — paprin is added in the amount of 15.7% by weight of the culture medium. The resulting composition is mixed and dried in a spray dryer at a baking temperature of 160 ° C at the inlet and 65 ° C at the outlet. As a result, 209.8 kg of bacilliquin 30, a standard antibiotic preparation containing 30 g of bacitracin per kilogram, is obtained from 1 m of culture medium.

5 product. The bulk of bacilliquin 30 is yeast biomass, the preparation as a whole is also characterized by the presence of a significant amount of protein — 48.1 May. % crude protein. Content, May. %:

0 amine nitrogen 0,4; carbohydrates 10.5, including reducing substances 0.4,

Cornmeal is added to the second sweat in the amount of 13% of the weight of the culture medium. The resulting composition is agitated, maintained at 55 ° C for 2 hours and then dried in a spray dryer under the above regime. 183.6 kg of bacilliquin 30 are obtained from 1 m3 of culture medium. The bulk of it is fermentolysate.

corn flour, the drug is generally characterized by the presence of significant amounts of easily digestible sugars. Content, May,%: crude protein 15.8; amine nitrogen 0,3; carbohydrates 66.4, including reducing substances 6.0.

The third stream (control) is evaporated in a vacuum evaporator at 65 ° C for 1 h, dried in a spray dryer under the same conditions, and the product obtained by dehydrating 1 m of culture medium, after grinding in a hammer crusher, is mixed with 108.3 kg of corn flour. As a result, 166.6 kg of bacillinus 30 is obtained from 1 m of culture medium. At the same time, from 1 m3 of culture medium, 0.67 m of condensate with a COD value of 48 mg / l 02 is received in the wastewater. case is corn flour without fermentolysis products. Content, May. %: crude protein 16.1; amine nitrogen 0,1; carbohydrates 59.3, including reducing substances 1.2.

PRI me R 3. Producer of the antibiotic tylosin - Streptomyces fradiae, strain 3830, cultured at a temperature of 28 ± 1 ° C, pH 6.8-7.0 and aeration 38 g 02 per 1 m3 of medium per minute for 200 h on next Wednesday, May. %: Soybean flour 2.5; glucose 2,5; corn extract 1.0; salt 0.2; chalk 0.5, propynol B-400 0.2; water plumbing rest. The resulting culture medium with tylosin is divided into three equal flows.

An association of bacteria, yeast, fungi, microalgae and protozoa (activated sludge) is added to the first stream in the form of a 17% suspension in the amount of 33.6% dry matter by weight of the culture medium. The resulting composition is stirred, maintained at 39 ° C for 5 hours and then dried in a spray dryer at a heat carrier temperature of 135 ° C at the inlet and 60 ° C at the outlet. As a result, 368 kg of randomisin 5, a standard antibiotic preparation with a content of 5 g of tylosin per 1 kg of product, is obtained from 1 m of culture medium. The bulk of randomisin 5 is activated sludge fermenter, the preparation as a whole is also characterized by the presence of a significant amount of easily digestible protein substances. Content, May. %: crude protein 43,6; amino nitrogen 2,3; carbohydrates 11.8, including reducing substances 2.1.

In the second stream, the bacteria biomass is added - haprin with a humidity of 5.8 May. %

in the amount of 34.9% by weight of the culture medium. The resulting composition is stirred, maintained at 20 ° C for 15 h and then dried in a spray dryer under the above mode. As a result, 361 kg of randomisin 5 is obtained from 1 MJ culture medium. The bulk of it is fermented bacteria biomass of bacteria, the preparation as a whole is also characterized by a significant amount of easily digestible proteins, May. %: crude protein 57.9; amine nitrogen 3,5; carbohydrates 13.7, including reducing substances 0.8.

The third stream (control) is evaporated in a vacuum evaporator at 60 ° C for 1 h, dried in a spray dryer under the above regime and the product obtained by dehydrating 1 m3 of culture medium, after grinding in a hammer mill, is mixed with 312.4 kg Hapriin with a humidity of 6.8 May. % As a result, 344 kg of randomisin 5 is obtained from 1 m of culture medium. At the same time, from 1 m3 of culture medium, 0.64 m3 of condensate with a COD of 53 mg / l Oa was fed into the wastewater. The bulk of individualisin 5 in this case is the biomass of bacteria without its fermentolysis products. May contents. %: crude protein 75.4; amino nitrogen 1,1; 13.5 carbohydrates, including reducing substances 0.3.

As can be seen from the above descriptions, according to the proposed method, up to 25.9% of the antibiotic is obtained additionally by the simplified technology (example 2). The method allows, along with the fractions of the culture medium, to utilize the wastewater treatment product, water, activated sludge. The resulting preparations are capable of enriching the feed not only with antibiotics, but also with proteins, carbohydrates and easy-to-digest products of their hydrolysis.

Claims (2)

1. A method of obtaining feed antibiotics, including biosynthesis of the target product in a liquid nutrient medium, separation of the culture medium, drying, introduction of a filler, characterized in that in order to simplify the method and reduce the loss of the antibiotic, the filler is introduced into the culture medium in an amount of 3-67 May . % 2. Method pop, 1, characterized in that microorganism biomass or activated sludge is used as a filler,