SU1668387A1 - Strain of bacterium vibrio nag serovara, used for preparation of monovaric diagnostic serum - Google Patents

Abstract

This invention relates to the field of microbiology, namely to the serological identification of pathogenic vibrios. The aim of the invention is a serovar strain VIBRIO NAG 079, which is used to prepare a monovarial diagnostic serum for the identification of pathogenic vibrios. Strain VIBRIO NAG KM - 49 (203) 079 serovar has all the morphological, cultural and physiological properties characteristic of the genus VIBRIO. Strain KM-49 (203) has a specific O-antigen, and specific antibodies are formed by immunizing animals. The resulting serum agglutinates the vibrios of only a homologous 079 serovar and does not react with the vibrios of heterologous serovars.

Description

This invention relates to the field of microbiology, namely to the serological identification of pathogenic vibrios.

The aim of the invention is a serovar Vibrio Nag 079 strain suitable for preparing a monovarial diagnostic serum for identifying pathogenic vibrios.

The bacterial strain Vibrio Nag 079 serovar deposited in the museum of living cultures of the All-Union Anti-Plague Research Institute of Microbe under the number CM-49 (203).

The strain Vibrio Nag KM-49 (203) has all the properties characteristic of the Vibrio genus. Morphological signs. On dense agar forms a smooth round-with a smooth edge of the transparent colonies with a diameter of 2-2.5 mm. In liquid nutrient media causes a uniform turbidity, forms a film on the surface.

Physiological properties. Oxidase-positive, ferments glucose and mannitol (up to acid without gas), does not ferment inositol and salicin, produces indole and hydrogen sulfide, belongs to the Heiberg group I, does not phosphoresce, gives rise to the phenomenon of torso, decarboxylates lysine and ornithine, and does not dehydrate arginine , does not grow in 1% peptone water with the addition of 7 and 10% sodium chloride. The Vibrio Nag KM-40 (203) strain is agglutinated by cholera diagnostic 0 serum at a dilution of 1: 100 and is not agglutinated by cholera diagnostic sera Inaba, Ogawa, PO. Not lysed by cholera diagnostic phages C, Eltor, HDF 3, 3, 5, and TEPV bacteriophages 1-7.

It has a specific 0-antigen. When animals are immunized with the 0 antigen of this strain, specific antibodies are formed.

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Example 1. For the preparation of the mono-tritic bottom of the 079 serum 070 seed serum, the Vibrio Nag KM-49 (203) 0 antigen was used. The strain culture is sown in 1% peptone water, pH 7.6 and grown at 37 ° C for 4 h, then subcultured on freshly mown agar containing infusion of cardiac muscle, pH 7.6. Crops are incubated at 37 ° C for 18-20 hours. From the surface of the agar, the culture is washed off with a physiological solution pH 7.2. The resulting microbial suspension is boiled at 100 ° C for 2 hours from the time it is boiled in a 10% solution of sodium chloride. The suspension is then washed twice with a physiological solution pH 7.2 by centrifugation. After controlling for specific sterility, microbial suspension is used; coziness to immunize animals.

Example 2. Chinchilla rabbits weighing 2-2.5 kg are immunized three times at three-day intervals: the first and second injections are given subcutaneously in 20 milliard each. mt, the third - intravenously 5 billion mt On the seventh day after the last injection, the rabbits are exsanguinated. Serum canned by the addition of sodium merthiolate 1: 10,000, Titres

The cost is 1: 3200. For a diagnostic titer, the dilution was 1: 400.

Serum is used in the agglutination reaction with vibrios released from

patients with acute intestinal diseases of people. The agglutination reaction is made using the volumetric method in test tubes, and the serum is used at the same dilution as a diagnostic titer. For serum dilution

and preparing culture suspensions using a physiological solution pH 7.2. Positive consider the reaction with a rating of 2-4 +.

Anti-Vibrio Serum

079 serovar is strictly specific and highly active. A positive reaction is noted only with 079 serovar vibrios.

This serum is of practical importance for microbiological

diagnosis of intestinal diseases. It allows for a wider differentiation of pathogenic vibrios.

Claims (1)

Claims of Invention Bacterial Strain Vibrio Nag KM-49 (203) serovar 079 used for cooking; laziness monovar diagnostic serum.