SU1666072A1 - Method for prognostication of gastroenteric diseases in newborn calves - Google Patents

Abstract

The invention relates to veterinary medicine. The aim of the invention is to increase the reliability and accuracy of prediction of diseases of newborn bodies. Blood samples are taken from newborn bodies to determine the level of immunoglobulins, and mother cows have samples of colostrum for the presence and content of analic acids. With a content of sialic acids on the background of a low level of immunoglobulins in the range of 2.5 to 12.0 mg / ml, an increased is predicted, and at 0.0-0.3 mg / ml, a high risk of gastrointestinal diseases in the body is predicted. Reliability of the method 70 - 100%. 2 tab.

Description

The invention relates to veterinary medicine.

The purpose of the invention is to increase the reliability and accuracy of the method.

Example. For the implementation of the method, samples of colostrum are taken in 0.5 ml each, 3.0 ml of 0.5 N NaOH is added to the samples of colostrum, shaken until a homogeneous suspension is obtained, 2.0 ml of 1N is added. Phase and hydrolyze in a boiling water bath for 20 minutes. The hydrolyzate is cooled. After cooling, samples of 0.5 ml are taken and the content of sialic acids (based on the Warren method) is determined using sodium meta-periodate and arsenite and a 0.6% aqueous solution of thiobarbituric acid in 0.5 M N aSO-fi.0 the presence of sialic acids is judged by the color of the sample of the test colostrum obtained during the reaction.

Based on chromatographic data, it was determined that the color of the sample depends on the content of sialic acids. By

The results of the studies compiled an analytical scale for determining the content of sialic acids, which does not require chromatography and spectrophotometry of the samples.

The scale for determining the content of sialic acids in colostrum samples of cows by the color of the chromogen after the color reaction is presented in Table 1,

M-acetylneiramine acids produced in England, Latvian SSR, as well as sialic acids, cleaved from u-human blood globulin, were used as a standard in the development of the scale.

The determination of the content of immunoglobulins in the blood serum of newborn calves is carried out by the express method using the sulphite-sodium test (Pfeiffer and Mac Gear). For this purpose, three solutions of sodium sulfite with cono are prepared.

oh oh h

I4d

14.0 concentrations; 16.0 and 18.0%. Take three tubes and pour 1.9 ml of the appropriate sodium sulfate solution into each tube, then add 0.1 ml of the test serum to each tube, mix thoroughly and incubate at room temperature for 1 The occurrence of sediment in a test tube with an 18% solution is indicated with a low content of immunoglobulins - less than 5.0 g / l (pronounced hypogammaglobulinemia).

When turbidity appears in test tubes with 18 and 16% solutions, the content of immunoglobulins is greater than 5.0 g / l, but less than 15.0 g / l. The appearance of turbidity or sediment in three test tubes (18; 16 and 14% solutions) indicates the content in the serum of 15.0 g / l of immunoglobulins. This indicator is taken as the norm.

Example 2. In 38 fresh cows, samples of colostrum were taken and examined for an Increased risk of gastrointestinal diseases was predicted, starting with the 111 group (the content of syllovy acids in colostrum was 12.0-2.5 mg / ml against a background of decreased availability and the content of sialic acids Blood samples were taken from newborn calves (38 heads) to determine the level of immunoglobulins.

Determination of sialic content

acids. In samples of colostrum (0.5 ml each), 3.0 ml 0.5N each was added, and NaOH was shaken until a homogeneous suspension was obtained, to which 2.0 ml 1N each was added. Н2ЭО4 and carried out hydrolysis in a boiling water bath for 20 min. The resulting hydrolyzate was used to conduct a color reaction (based on the method of Warren, 1959)

Determination of the level of immunoglobulins. Prepared 3 solutions of sodium sulfite with concentrations of 14.0; 16.0 and 18%. 1.9 ml of the corresponding solution of sodium sulfite was poured into each tube and 0.1 ml of the test serum was added, mixed thoroughly and kept at room temperature for 1 hour.

Based on the results of studies of the colostrum of mother cows and serum of newborn bodies, 5 groups were distinguished:

Group (4 heads)

Group II (4 heads)

Group III (13 goals)

Group IV (13 goals)

V group (4 heads)

immunoglobulin level), and a high risk in groups 4 and 5, respectively (sialic acid content 0.3-0.2 mg / ml and 0.0 against the background of a low level of immunoglobulins). Forecast confirmed (Table 2)

The incidence of calves, depending on the content of sialic acids in the colostrum of cows-mothers and the level of immunoglobulins in the blood serum of the bodies, is presented in table 2.

Prediction of the risk of gastrointestinal diseases in newborn bodies by the proposed method can be carried out under conditions of any host. The predictive accuracy and accuracy of forecasting are high. As a result of the color reaction, a regular relationship was established between the color of the sample; the molal content of sialic acids in colostrum was 30.0–17.0 mg / ml; the level of immunoglobulins in the blood serum of bodies was 15.0–5.0 g / l. The content of sialic acids in colostrum 25.0-15.0 mg / ml. The level of immunoglobulins in the blood serum of the bodies is 15.0-5.0 g / l The content of sialic acids in colostrum is 12.0-2.5 mg / ml, the level of immunoglobulins in the blood serum of the bodies is 15.0-5.0 g / l The content of sialic acids in colostrum - 0.3-0.2 mg / ml, the level of immunoglobulins in the blood serum of bodies 15.0-5.0 g / l The content of sialic acids - 0.0, the level of immunoglobulins in the blood serum of bodies - 15.0-5.0 g / l

Ziva after the color reaction and the content of sialic acids in it.

The resulting colored chromogenic complex does not require extraction with organic solvents (butanol, butyl acetate, cyclohexanone, etc.), which eliminates the need for the use of toxic solvents for the analytical reaction, an expensive spectrophotometric instrument. The stable color of the analytical reaction is sufficiently informative. This makes it possible to carry out the reaction at a convenient time for the investigator and to promptly predict the risk of gastrointestinal diseases in the body.

The proposed method allows to predict the occurrence of gastrointestinal diseases in tel in 70-100% of cases compared to 40.0-50% when analyzing only protein indicators.

Claims (1)

Claims of the invention A method for predicting gastrointestinal diseases of newborn bodies, including determining the level of immunoglobulins in the blood serum of newborn bodies, characterized in that. which, in order to increase  According to chromatographic analysis. Relative to the total amount of sialic acids and other sugar acids that react with reagents. reliability and accuracy due to the establishment of the degree of risk, the colostrum of mother cows is additionally examined for the content of og-keto-delta-amino-penta-hydroxycarboxylic (sialic) acids and their content against the background of low immunoglobulin levels within 2.5-12.0 mg / ml predict increased, and at 0.0-0.3 mg / ml - high risk of gastrointestinal diseases t. Table 1 table 2