Научно-исследовательская лаборатория биологически активных веществ гидробионтов Министерства здравоохранения СССР
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FIELD: medicine, biotechnology. SUBSTANCE: sturgeon fish spleens were homogenized in 0.9-1.1 M aqueous acetic acid solution at ratio raw and solution = (5:3)-(5:5) followed by extraction in the same solution. Obtained mixture is centrifuged at 3500 rev/min for 15 min at 0 C and precipitate is discarded. To supernatant 0.10-0.15 M aqueous ammonia solution is added up to pH = 7.0, kept at 8 C for 10 hr and solution is filtered and subjected for lyophilic drying. Yield of preparation is 32.5-34.5 g from 1 kg sturgeon spleen. EFFECT: increased specific activity and yield of product, simplified method.
SU4744466/14A1989-09-291989-09-29Method of preparing vestibuloprotecting agent from animal raw
SU1660250A1
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