SU1602538A1 - Chamber for incubation of brain section - Google Patents

Abstract

The invention relates to medical technology for maintaining the viability of brain slices in a specific environment. To extend the functionality and increase the duration of the viability of the brain slices, the chamber for incubating the surviving brain slices contains tanks: worker 1.2 and heat exchange 3, feed tubes 4, waste tank 5, optical glasses 9, support grid 11, clamping cylindrical rings 12, distributor the regulator 17, the thermostat 19, the source 20 of the gas mixture, the sprayers 21 of the gas mixture. 1 il.

Description

This invention relates to a medical technique for maintaining the viability of brain slices in a special environment.

The aim of the invention is to extend the functionality and increase the duration of the viability of brain slices,

The drawing shows schematically an incubation chamber for surviving brain sections.

The chamber contains the upper 1 and lower 2 compartments of the working tank 1, 2, heat exchange tank 3, upper and lower supply tubes 4, waste tank 5, level regulator 6 — outlet tube 7 in the working tank, flexible connecting tubes 8, optical glasses 9, air insulation 10, supporting grid. 11, the outer and inner clamping cylindrical rings 12, an extract 13 of the inner cylindrical ring, - the cover plate 14 with a hole for

electrodes, sealant tube 15, thermostat tube 16, distribute regulator 17, tank 18 of the incubation solution in a water bath ,.- Thermostat 19, gas mixture source 20, gas mixture sprayers 21.

The incubation chamber of the surviving brain slices works as follows.

From the incubation solution tank 18 in a water bath, pre-saturated with gas mixers 21, the initially heated solution enters the working reservoir 1.2 through feed tubes 4, passing through the distributor-regulator 17, which selects the operating mode of the chamber and the speed of the solution. In the case of working with whole submerged sections, the distributor-regulator, 17 is switched to the supply position through the additional upper supply tube 4 of the solution entering the upper compartment 1 of operation5

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of which the reservoir, in the case of working with immersed sections through this the tube enters the heat and is humidified with a gas mixture (95% C02) - coming from the source 20 of the gas mixture. Before entering the working tank 1, 2, the solution is reheated, passing through the feed tubes 4 in the region of the thermostat tubes 16 and the heat-transfer tank 3,. All heating is carried out from a liquid-circulating thermostat 19 o From the working tank 1.2, the solution enters the waste container 55 from where through the discharge tube 7c 5 with flexible connecting tubes 8 the solution enters the level regulator 6 in the working tank with an L-shaped tube from where the solution flows outward through the discharging of the spent solution. The brain slice is freely positioned on a support grid 11, fixed between the cylinder and the 1st cylindrical rings 12, which have coaxial holes in which 25 tube-compacted 5, No need to fix the slice is achieved by the annular protrusion 3 on the inner clamping ring 125 in the cyan area approximately equal to the thickness of the brain slice 5, which leads to the elimination of the face pressure of the fluid current to the brain slice. thermal insulation 10 between them. From above, the working reservoir 1.2 is covered with a transparent plate 14 with a hole for inserting electrodes with During the incubation process, the brain is either evenly sliced with an incubation solution on either side, or washed with a solution from the bottom, and aerated and heated with a moistened and heated gas mixture from above.

The advantage of the chamber for incubating the surviving brain slices is its versatility, which creates the possibility of incubating the brain slices with both fully immersed in the liquid and on the gas-liquid boundary.

Claims (1)

Invention Formula An incubation chamber for surviving brain slices, containing a working reservoir, a dummy net that separates the working reservoir into the upper and lower compartments, the incubation solution reservoirs with feed tubes and a gas mixture sprayer, a thermostatic system, and a waste tank, characterized in that in order to extend the functionality and increase the duration of the viability of brain slices, it is equipped with an additional supply tube connected to the upper compartment of the working reservoir, the distribution The regulator connecting the supply tubes with the incubation solution tank and the source of the gas mixture is controlled by a level regulator in the working tank communicated with the waste tank, clamping cylindrical rings of different diameters in the working tank on the support grid and optical glass installed in the lower compartment of the working tank nineteen W 4 if li 15113 J2 12 5