SU1601629A1 - Method of modeling toxico-allergic uveitis - Google Patents

Abstract

The invention relates to medicine, in particular to ophthalmology, and can be used in experimental studies of uveitis. The aim of the invention is to reduce the time required to obtain a model. The goal is achieved by reintroducing the resolving dose of horse serum in an amount of 0.1-0.2 ml into the anterior chamber of the rabbit eye after treating the inflammatory process in the vascular membrane of the eye with clinical recovery. The method is easy to perform technically, safe for the whole body. The resulting model of the inflammatory process in the vascular tract of the eye is demonstrative and has a severe course.

Description

. This invention relates to experimental medicine and can be applied in simulating uveitis.

The purpose of the invention is to reduce the time of receipt of the model.

The method is carried out as follows.

In rabbits, they cause toxic-allergic uveitis after sensitization of the rabbit's body by injecting sterile normal horse serum into the marginal ear vein in the amount of 1.0 ml for 5 days at intervals of 24 hours. After 10–13 days, during the peak of raising antibodies to this antigen, a resolving dose of serum is introduced into the eye in an amount of 0.1-0.2 ml. Experiments carried out on the right eye to the left serves as a control. After 3-4 days, an inflammatory process develops in the vascular tract of the eye block with varying degrees of severity. After this, local treatment of experimental uveitis is carried out. After the verse of the postesan process, 0.1-0.2 ml of serum is reintroduced into the anterior chamber, starting from the volume of the anterior chamber moisture and literature data on the introduction of antigens into the anterior chamber to obtain experimental uveitis. The next day, after repeated administration, a jelly tox develops; -1 co-appeler; 1 uveitis.

Example 1. Rabbit No. 17, weight 2600 g. From January 29, 1987, to 03/02/87, the body of the animal was sensitized with 1.0 ml of sterile normal horse serum without preservatives by introducing a rabbit ear into the marginal vein.

02/16/87, a resolving dose of serum in the amount of 0.1 ml was introduced into the anterior chamber of the right eye, the left one served as a control.

02/19/87, the height of the primary toxic-allergimes clinic at her was observed. Mixed injection of the eyegia block.

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fPoroBHua is swollen. Precipitates on the corneal endothelium and on the anterior capsule of the crystal - 4ik. The exudate in the anterior chamber level (height) up to 2 3 mm. The iris is hyperemic and changed in color. Posterior synechia for 3 and 5 h. In the vitreous body there is a clear opacity. A slightly pink reflex was detected from the fundus of the eye, 1 Local toxico-allergic uveitis was treated by instillation of 30% sodium sulfacyl solution 3 times, 0.25% scopolamine solution 1 time, dexamethasone solution 2 times a day . • 03/03/87 - on the 12th day from the beginning of the development of uveitis, a complete cupping occurred - the first inflammatory process in the vascular cap of the eye block. The eye is calm. On the upper half of the cornea, a gentle cloud is visible turbidity. The front camera is of medium depth, moisture is transparent. Iris | calm. The pupil is wide, cool. The eye- JHoe bottom ophthalmoscopy well, the details are clearly visible. j 03/05/87. The 14th day since the beginning of the development of Iveitis, was re-allowed, and the 1 dose of antigen into the anterior chamber of the eye in: 0.1 ml dose.

03/06/87, on the first day after the injection, neither a repeated resolving dose of serum into the anterior chamber of the eye I developed a pronounced clinic of secondary toxico-allergic uveitis. Chemoz conjunctiva. Expressed mixed injection of the eye block. The cornea is sharply swollen, dim. In the anterior chamber, the level of hypopyon is 5-6 mm. The iris is drastically changed in color and pattern. Pupil 3x2 mm, rigid. In the vitreous body exudate.

03/07/87, the exudation of the eye's eye intensified. Hypopiomas occupies the entire anterior chamber of the eye,

03/25/87, the 20th day from the beginning of the experimental uveitis. The inflammation subsided somewhat. Hypotonies. Mixed eye block injection. Corneal opacity. Exudative film on the front surface of the iris. The posterior eyes are not visible.

03/30/87 - keeps the injection of the eye block mixed. Cutting hypotonia. Subatrophy eye block. The cornea is cloudy. Atrophic changes in the iris are hardly determined. Pupillary edge spliced with the lens. From the fundus, the reflex is not detected,

04/06/87 - the eye is calm. The hypotony, the subatrophy of the eye, the low-grade reflex is determined from the fundus of the eye.

Example 2, Rabbit number 2 1, weight 3000 g

From 01/29/87 to 03/02/87, the body of the animal was sensitized with 1.0 ml of horse serum by injecting 24 hours into the marginal vein of the rabbit ear.

16.02.87, the allowing dose of serum antigen in the amount of 0.2 ml was introduced into the anterior chamber of the right eye, the left one served as a control.

019.02.87 was the height of the clinic

primary toxic-allergic uveitis. Expressed mixed injection of the eye block. The cornea is swollen, on the endothelium its large precipitates. Exudate in front of the 5 chamber height up to 4 mm. Posterior synechia from 5 to 9 h. In the anterior section of the vitreous body, turbidity is determined. A low-borne reflex is detected from the fundus.

Local treatment of primary toxic-allergic uveitis was performed by instillation with a solution of sodium sulphacyl 307o, 0.25% scopolamine, and dexamethasone.

04.03.87 g. - on the 13th day from the beginning of the development of uveitis, a complete reduction of the inflammatory process in the choroid of the eye block occurred. The eye is calm. Cloudy clouding, clouding of the cornea. The front camera is of medium depth, moisture is transparent. The iris is 0 coyne. The pupil is wide, of irregular shape, for 6 hours there is posterior synechi. There is a gentle floating turbidity in the vitreous. The fundus of the eye is ophthalmoscopically, the details are not changed.

3505,03,87 g, 14-day since the beginning of development

uveitis, re-resolving dose of antigen was injected into the anterior chamber of the eye at a dose of 0.2 ml,

06,03,87 g, - on the first day after the introduction of a repeated resolving dose of antigen into the anterior chamber of the eye, a clinic of secondary (recurring 1, eg) toxic-allergic uveitis developed. Severe inflammatory process in the vascular 45 sheath of the eye block was accompanied by signs of alteration of the eye block walls. Chemoz conjunctiva. The cornea is sharply swollen, infiltrated, dim. The front chamber is completely filled with 50 exudates. The posterior eyes are not visible. Bright red color mixed injection of the eye block.

11.03.87 g, fifth day since the beginning of the clinic for secondary toxic-allergic uveitis. Corneal swelling was somewhat asleep. The exudate of the anterior chamber has a height of up to 6 7 mm, t, e, covers the pupillary area. The visible part of the iris is sharply G11G “rvmi), changed in color, covered with a j ccy / ia-aive film.

03/21/87 - on the 15th day from the beginning of the development of uveitis, corneal edema was asleep, there is turbidity in it. Exudate height up to 3 - 4 mm in the anterior chamber. The pupil of 3 X 3 mm is covered with exudative film. The iris is still hyperemic. From the fundus, the reflex is undetectable.

04/01/87 - the injection of the eye block has decreased.

Thus, the proposed model of a toxic-allergic inflammatory process in the vascular tract of the eye block is a demonstrative, severe form of uveitis, simple in technique and safe for the whole body.

According to available data, toxic-allergic uveitis with a severe course, mixed injection of the eyeblock, corneal swelling, multiple precipitates, anterior exudation in the anterior chamber, posterior synechi, inflammatory changes in the iris and exo

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sucking into the vitreous. Of the 24 rabbits, 20 (83.3%) had a severe uveitis, 4 (16.6%) had an average weight.

The mode of modeling is safe for the whole organism, since the rabbits remained alive while observing them for 1.5 months from the day the repeated resolving dose of antigen was introduced into the anterior chamber of the eye.

The course of toxic-allergic uveitis resembles the course of recurrent severe uveitis in the clinic, which are based on impaired immunological homeostasis and sensitization of the organism.

Claims (1)

The invention of the method of modeling toxic-allergic uveitis by injecting horse serum into the eye of an experimental animal, characterized in that, in order to shorten the time for obtaining the model, a subsequent local treatment is performed, then after recovery, 0.1 to 0.2 ml of serum is reintroduced .