SU1558983A1 - Method of preparing nutrient substrate for yeast growing - Google Patents

Abstract

The invention relates to the microbiological industry and is a method for preparing a nutrient substrate based on a hydrolyzate of plant raw materials for growing yeast. The purpose of the invention is to increase the yield of yeast. The hydrolyzate of plant materials is blown off with steam and is processed in a known manner. The furfural-containing steam is condensed, the concentration of furfural is determined in the condensate and treated with ozone in an amount of 0.2-0.3 of the amount of furfural. The hydrolyzate is mixed with the ozone-treated condensate in a ratio of 1: (0.05-1) and used as a substrate for growing yeast. 3 tab.

Description

The invention relates to the microbiological industry, in particular to a method for preparing a nutrient substrate based on a hydrolyzate of plant raw materials for growing yeast.

The purpose of the invention is to increase the yield of yeast.

The method is carried out as follows.

One of the stages of preparation of the nutrient substrate from the hydrolyzate of plant raw materials is purging with steam, which is subjected to the hydrolyzate before or after treatment in a known manner, including neutralization, crystallization and release of gypsum. After cooling, the vapor forms furfural-containing condensate, in which the concentration of furfural is determined, after which the condensate is treated with ozone, the amount of ozone being 0.2-0.3 of the amount of furfural in the condensate.

The clarified hydrolyzate is mixed with the ozone-treated condensate in the ratio l: (0.05-l) and used as a substrate for growing yeast. During the ozonation of furfurolsoda of condensing condensate, furfural is deeply oxidized with the formation of volatile organic acids (LOK), which serve as an additional carbon food for yeast.

Example 1. The hydrolyzate obtained by hydrolysis of a mixture of needles and hardwood at 180 ° C was blown with steam at 100 ° C for 2 hours. Furfural containing steam was condensed. The amount of condensate was 50% of the amount of blown hydrolyzate. Condensate with a furfural concentration of 0.11% and LOK 0.085% were treated with ozone at doses of 0.15; 0.22; 0.33; 0.4 g / l.

Purified from furfural, the hydrolyzates were neutralized with lime milk to pH 3.5 and the gypsum was crystallized at 90 ° C for 1 hour. The gypsum was separated by settling. The clarified neutralizer was neutralized with ammonia water to pH 4.2, aerated at 80 ° C for 2 hours, cooled to 40 ° C, and suspended particles were separated by settling.

The prepared neutralite was mixed with ozonated condensate, mineral salts and Candida Scotti yeast were added at 38-39 ° C. The results are presented in table. I.

Example 2. The wood hydrolyzate was neutralized and gypsum was separated as in Example 1 and a cooling unit was applied to the vacuum, where the neutralized agent was cooled to 40 ° C. Self-evaporation vapors from the vacuum-evaporator unit were condensed and mixed with the condensate vapor obtained from the evaporators upon cooling the hydrolyzate from 180 to 100 C. The amount of condensate was 15% of the amount of hydrolyzate. The concentration of furfural in the condensate is 0.3%, the concentration of LOK is 0.24%.

After removal of furfural, the neutralization product was aerated for 1 h, the suspended substances were separated by settling and mixed with the condensate, which was previously ozonated, 10

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Wali doses of 0.5; 0.6; 0.9; 1.0 g / l. - The results are presented in Table. 2

Example 1. The wood hydrolyzate was neutralized and gypsum was isolated as in Example 1 and was fed to a vacuum distillation column with a bottom heat exchanger. The vapors after the vacuum distillation column were condensed. The amount of condensate was 5% of the amount of neutralized. The concentration of furfural in the condensate is 2.0%, the concentration of LOK is 1.3%.

The condensate was treated with ozone with doses of 3.5; 4.0; 6.0; 7.0 g / l and mixed with neutralized, prepared as in example 1, the Results are presented in table. 3

Thus, the proposed method allows to increase the yield of yeast by 7-35% in comparison with the known method, as well as to create a waste-free technology for producing a nutrient substrate.

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Claims (1)

Invention Formula A method of preparing a nutrient substrate for growing yeast, involving the hydrolysis of vegetable raw materials, blowing with steam followed by the production of furfural-containing condensate, neutralizing the hydrolyzate, crystallization and release of gypsum, cooling, separation of solids, characterized in that, to increase the yield of yeast, after separation solids The hydrolyzate is mixed in a ratio of 1: 0.05-1 with furfurol-containing condensate, pre-treated with ozone, while the amount of ozone is 0.2-0.3 on the amount of furfural in the condensate.