SU1493673A1 - Tinting agent for fluorescent microscopy of bacterial cells deposited on membrane filter - Google Patents

Abstract

This invention relates to the microbiological, pharmaceutical, and electronic industries. The purpose of the invention is to increase the contrast of the image of bacterial cells in the light of luminescence during microscopy. The dye for fluorescence microscopy of cells deposited on a membrane filter contains black mascara, water, polyatomic alcohol and non-ionic surfactant (SAS) in the following ratios of components, wt. P .: black mascara 1-1.5

water 1-1,5

alcohol 1-3

nonionic surfactant 0.058-0.13. As a nonionic surfactant, the dye includes octylphenoxypolyethoxyethanol, octylpolyethoxyethanol, phenoxypolyethoxyethanol, and as a polyhydric alcohol - glycerin, ethylene glycol, propylene glycol. The dye may additionally contain fluorochrome for nucleic acid cells at a concentration of 10 ^-3 -10 ^-4 wt.h. 3 hp f-ly.

Description

 The invention relates to microbiological research, in particular to luminescence microscopy, and can be applied in the pharmaceutical, microbiological, electronic industry and environmental monitoring.

The purpose of the invention is to increase the contrast of the image of cells in the light of luminescence.

This goal is achieved by adding a non-ionic surfactant and a polyhydric alcohol to the dye.

Example 1. Dye composition for membrane filters from a mixture

cellulose ethers from Millipore,

ma.ch.

0.2 μm pore diameter — ink-1, water-1, syntanol-0.128, glycerol-2;

pore diameter of 0.45 µm - ink-1, water, 1,

syntanol-0.08, glycerol-2; mascara-1, water, 1, syntanol-0.08; glycerol-3; mascara-1.5, water-1.5, syntanol-0, 08, glycerin-1; mascara-1,

 with

with

05

WITH

water-1, sintanol-0.08, propylene glycol-2; ink-1, water-1, f tritonkh-100- 0.08, ethylene glycol-2.

And p m ime R 2. The composition of the dye l membrane filters from .sansano- fQ th film, mac.ch .:

pore diameter 0.5 μm - ink-1, water-1,

syntanol-0.058, glycerol-2;

pore diameter 0.2 μm - ink-1, water-1, 15

sintanol-0,088, glycerin-2; mascara-1, water-1, syntanol-0.088, glycerin-2, these- 20 dium bromide-3x10; mascara-1, water-1, syntanol-0, 088, glycerin-2, acryne-25 dine orange - 3x10; ink-1, water-1, phenoxypolyetho-xethanol-0,088, 30 glycerin-2, acridine orange - 3x10.

II p and me R 3. The proposed dye is used as follows. ,, and a glass slide drip 4 drops of dye. Then, a dried filter with cells precipitated on it is placed on the dye (the side with the cells is up), incubated for 20-30 minutes and matched in a luminescent microscope. Cells are observed as light

parts1 .1 on a black background. When cells are stained with a fluorochrome containing dye, the cells acquire a specific color and can be easily distinguished from impurity particles that are not stained by fluorochrome.

Claims (4)

1. A dye for fluorescence microscopy of bacterial cells deposited on a membrane filter, including black ink and water, characterized in that, in order to increase the image contrast, the dye further comprises | polyhydric alcohol and non-ionic surfactant I in the following ratio of components, wt.%: Black ink 1.0-1.5 Water1.0-1.5 Non-ionic PAV0,058-0,13 Polyatomic alcohol1.0-3.0 2. The dye of claim 1, wherein as the non-ionic surfactant it contains either octylphenoxypolyethoxyethanol, or octylpolyethoxyethanol, or phenoxypolyethoxyethanol. 3. The dye according to claims 1 and 2, o t - deprived of the fact that it contains either glycerin, or ethylene glycol or propylene glycol as a polyhydric alcohol. 4. The dye of claims 1 to 3, about tl, and - by the fact that it additionally contains fluorochrome for nucleic acids in concentrations of 10 to 10 parts by weight.