SU1490962A1 - Способ встраивания днк в геном аденовируса и рекомбинантный аденовирусный вектор celo/puc 19 - Google Patents

Способ встраивания днк в геном аденовируса и рекомбинантный аденовирусный вектор celo/puc 19

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Publication number
SU1490962A1
SU1490962A1 SU4264178/13A SU4264178A SU1490962A1 SU 1490962 A1 SU1490962 A1 SU 1490962A1 SU 4264178/13 A SU4264178/13 A SU 4264178/13A SU 4264178 A SU4264178 A SU 4264178A SU 1490962 A1 SU1490962 A1 SU 1490962A1
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USSR - Soviet Union
Prior art keywords
genome
celo
dna
adenovirus
fragments
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Application number
SU4264178/13A
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English (en)
Inventor
В.И. Грабко
К.А. Бендукидзе
И.И. Фодор
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Институт Биохимии И Физиологии Микроорганизмов Ан Ссср
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Priority to SU4264178/13A priority Critical patent/SU1490962A1/ru
Application granted granted Critical
Publication of SU1490962A1 publication Critical patent/SU1490962A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10211—Aviadenovirus, e.g. fowl adenovirus A
    • C12N2710/10241—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10243—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Изобретение относится к биотехнологии, в частности к генетической инженерии, и позволяет получить рекомбинантный вектор - аденовирус CELO/pUC 19. С целью упрощения способа встраивания ДНК в геном аденовируса проводят гидролиз рестриктазой HbaI ДНК генома аденовируса CELO и плазмидной ДНК и неполный гидролиз рестриктазой Bgli ДНК генома аденовируса CELO затем смешивают Bgli фрагменты ДНК, содержащие 39% левой части генома аденовируса и два Xbal-фрагмента аденовируса CELO, содержащие 33-100% генома аденовируса CELO, которые предварительно последовательно лигированы в XbaI сайте на 94% генома с линейной плазмидной ДНК pUC 19 смесью, указанных фрагментов проводят трансфекцию клеток аллантоисной оболочки куриных эмбрионов. В результате рекомбинации in vivo в куриных эмбрионах из двух перекрывающихся фрагментов образуется полноценный геном Ad CELO содержащий в несущественной области гетерологическую ДНК - плазмиду pUC 9. 2 с.п. ф-лы.
SU4264178/13A 1987-06-17 1987-06-17 Способ встраивания днк в геном аденовируса и рекомбинантный аденовирусный вектор celo/puc 19 SU1490962A1 (ru)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU4264178/13A SU1490962A1 (ru) 1987-06-17 1987-06-17 Способ встраивания днк в геном аденовируса и рекомбинантный аденовирусный вектор celo/puc 19

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU4264178/13A SU1490962A1 (ru) 1987-06-17 1987-06-17 Способ встраивания днк в геном аденовируса и рекомбинантный аденовирусный вектор celo/puc 19

Publications (1)

Publication Number Publication Date
SU1490962A1 true SU1490962A1 (ru) 1995-04-10

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ID=60528074

Family Applications (1)

Application Number Title Priority Date Filing Date
SU4264178/13A SU1490962A1 (ru) 1987-06-17 1987-06-17 Способ встраивания днк в геном аденовируса и рекомбинантный аденовирусный вектор celo/puc 19

Country Status (1)

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SU (1) SU1490962A1 (ru)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6841158B1 (en) * 1998-09-22 2005-01-11 Boehringer Ingelheim International Gmbh Recombinant celo virus and celo virus DNA
EP1650310A1 (en) * 1993-04-14 2006-04-26 Commonwealth Scientific And Industrial Research Organisation Recombinant avian adenovirus vector

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1650310A1 (en) * 1993-04-14 2006-04-26 Commonwealth Scientific And Industrial Research Organisation Recombinant avian adenovirus vector
US6841158B1 (en) * 1998-09-22 2005-01-11 Boehringer Ingelheim International Gmbh Recombinant celo virus and celo virus DNA

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