SU1490962A1 - Способ встраивания днк в геном аденовируса и рекомбинантный аденовирусный вектор celo/puc 19 - Google Patents
Способ встраивания днк в геном аденовируса и рекомбинантный аденовирусный вектор celo/puc 19Info
- Publication number
- SU1490962A1 SU1490962A1 SU4264178/13A SU4264178A SU1490962A1 SU 1490962 A1 SU1490962 A1 SU 1490962A1 SU 4264178/13 A SU4264178/13 A SU 4264178/13A SU 4264178 A SU4264178 A SU 4264178A SU 1490962 A1 SU1490962 A1 SU 1490962A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- genome
- celo
- dna
- adenovirus
- fragments
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10211—Aviadenovirus, e.g. fowl adenovirus A
- C12N2710/10241—Use of virus, viral particle or viral elements as a vector
- C12N2710/10243—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Изобретение относится к биотехнологии, в частности к генетической инженерии, и позволяет получить рекомбинантный вектор - аденовирус CELO/pUC 19. С целью упрощения способа встраивания ДНК в геном аденовируса проводят гидролиз рестриктазой HbaI ДНК генома аденовируса CELO и плазмидной ДНК и неполный гидролиз рестриктазой Bgli ДНК генома аденовируса CELO затем смешивают Bgli фрагменты ДНК, содержащие 39% левой части генома аденовируса и два Xbal-фрагмента аденовируса CELO, содержащие 33-100% генома аденовируса CELO, которые предварительно последовательно лигированы в XbaI сайте на 94% генома с линейной плазмидной ДНК pUC 19 смесью, указанных фрагментов проводят трансфекцию клеток аллантоисной оболочки куриных эмбрионов. В результате рекомбинации in vivo в куриных эмбрионах из двух перекрывающихся фрагментов образуется полноценный геном Ad CELO содержащий в несущественной области гетерологическую ДНК - плазмиду pUC 9. 2 с.п. ф-лы.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU4264178/13A SU1490962A1 (ru) | 1987-06-17 | 1987-06-17 | Способ встраивания днк в геном аденовируса и рекомбинантный аденовирусный вектор celo/puc 19 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU4264178/13A SU1490962A1 (ru) | 1987-06-17 | 1987-06-17 | Способ встраивания днк в геном аденовируса и рекомбинантный аденовирусный вектор celo/puc 19 |
Publications (1)
Publication Number | Publication Date |
---|---|
SU1490962A1 true SU1490962A1 (ru) | 1995-04-10 |
Family
ID=60528074
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU4264178/13A SU1490962A1 (ru) | 1987-06-17 | 1987-06-17 | Способ встраивания днк в геном аденовируса и рекомбинантный аденовирусный вектор celo/puc 19 |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU1490962A1 (ru) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6841158B1 (en) * | 1998-09-22 | 2005-01-11 | Boehringer Ingelheim International Gmbh | Recombinant celo virus and celo virus DNA |
EP1650310A1 (en) * | 1993-04-14 | 2006-04-26 | Commonwealth Scientific And Industrial Research Organisation | Recombinant avian adenovirus vector |
-
1987
- 1987-06-17 SU SU4264178/13A patent/SU1490962A1/ru active
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1650310A1 (en) * | 1993-04-14 | 2006-04-26 | Commonwealth Scientific And Industrial Research Organisation | Recombinant avian adenovirus vector |
US6841158B1 (en) * | 1998-09-22 | 2005-01-11 | Boehringer Ingelheim International Gmbh | Recombinant celo virus and celo virus DNA |
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