SU1464694A1 - METHOD FOR DETERMINING IMMUNE COMPLEXES - Google Patents

METHOD FOR DETERMINING IMMUNE COMPLEXES

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Publication number
SU1464694A1
SU1464694A1 SU4156336/13A SU4156336A SU1464694A1 SU 1464694 A1 SU1464694 A1 SU 1464694A1 SU 4156336/13 A SU4156336/13 A SU 4156336/13A SU 4156336 A SU4156336 A SU 4156336A SU 1464694 A1 SU1464694 A1 SU 1464694A1
Authority
SU
USSR - Soviet Union
Prior art keywords
immune complexes
immunosorbent
removal
determining
determining immune
Prior art date
Application number
SU4156336/13A
Other languages
Russian (ru)
Inventor
А.И. Гриценко
Т.Ф. Кошецян
Л.А. Морозова
А.Б. Смирнов
Ж.А. Шажко
Original Assignee
Всесоюзный научно-исследовательский ящурный институт
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Всесоюзный научно-исследовательский ящурный институт filed Critical Всесоюзный научно-исследовательский ящурный институт
Priority to SU4156336/13A priority Critical patent/SU1464694A1/en
Application granted granted Critical
Publication of SU1464694A1 publication Critical patent/SU1464694A1/en

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Abstract

Способ определения иммунных комплексов, включающий получение иммуносорбента путем фиксации антигена или антитела на матрице, инкубацию иммуносорбента со специфическими антителами или антигенами с последующими удалением непрореагировавших компонентов и учетом результатов с помощью флуориметрии, отличающийся тем, что, с целью упрощения способа, в качестве матрицы используют частицы аэросила А-300 размером 4 - 40 нм, фиксацию осуществляют путем адсорбции в 0,1 - 0,05 М ацетатном буфере с рН 7,5 - 7,6, удаление неадсорбированных компонентов осуществляют забуференным физиологическим раствором с рН 7,5 - 7,6, содержащим 0,04 - 0,06% раствора твина-20.The method of determining the immune complexes, including obtaining immunosorbent by fixing the antigen or antibody on the matrix, incubating the immunosorbent with specific antibodies or antigens, followed by removal of unreacted components and taking into account the results using fluorimetry, in order to simplify the method, particles Aerosil A-300 4–40 nm in size, fixation is carried out by adsorption in 0.1–0.05 M acetate buffer with a pH of 7.5–7.6, removal of non-adsorbed components lyayut buffered saline at pH 7.5 - 7.6 comprising 0.04 - 0.06% Tween-20 solution.

SU4156336/13A 1986-12-04 1986-12-04 METHOD FOR DETERMINING IMMUNE COMPLEXES SU1464694A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU4156336/13A SU1464694A1 (en) 1986-12-04 1986-12-04 METHOD FOR DETERMINING IMMUNE COMPLEXES

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU4156336/13A SU1464694A1 (en) 1986-12-04 1986-12-04 METHOD FOR DETERMINING IMMUNE COMPLEXES

Publications (1)

Publication Number Publication Date
SU1464694A1 true SU1464694A1 (en) 2000-03-27

Family

ID=60534660

Family Applications (1)

Application Number Title Priority Date Filing Date
SU4156336/13A SU1464694A1 (en) 1986-12-04 1986-12-04 METHOD FOR DETERMINING IMMUNE COMPLEXES

Country Status (1)

Country Link
SU (1) SU1464694A1 (en)

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