SU1454845A1 - Strain of eremothecium ashbyi fungus as producer of essential oil - Google Patents

Abstract

The invention relates to biotechnology, essential oil production. The strain of the fungus Eremothecium ashbyi VKM F 3009D is used to produce the essential oil. The purpose of the invention is the isolation of a strain of the fungus Eremothecimn ashbyi VKM F 3009D, which has a higher biosynthetic activity. Cultural, morphological and physiological and biochemical characteristics. Dichotomously branching mycelium consists of many nuclear cells. The diameter of the hyphae varies from 2.5 to 16.5 microns. Sporangia oblong. Mnogosporovye. Sizes of ascospores: length 20.2-26.7 microns, diameter 2.5-2.8 microns. On the agar medium, it forms flat, opaque (later glossy) colonies of yellow color, easily removable from the agar. The shape of the colonies is round, diameter is 8-12 mm (on wort agar after 3 days of growth at 20 C). Uses glucose, fructose, raffinose, sucrose, glycerin, acetate and pitrate as carbon sources. Does not digest starch, pelg lyulose, ethanol, inositol. Coagulates milk, slightly dilutes gelatin. Synthesizes the essential oil, which includes geraniol, citronellol, nerol, Jb-phenylethanol, etc. The culture maturation time is reduced by 27%, and the yield of essential oil reaches 200-280 mg / l, which is 3 times higher than that known strain. Table I and sl 4 SP N {00 4 ate

Description

one

The invention relates to biotechnology, essential oil production, and represents a new strain of microscopic fungus used to produce essential oil.

The purpose of the invention is to isolate a strain of the fungus Eremothecium ashbyi VKM F 3009D - an essential oil producer.

possessing higher biosynthetic activity.

The proposed strain obtained by breeding.

The resulting strain of the fungus Eremothecium ashbyi BCM F 3009D is characterized by the following cultural-morphological and physiological-biochemical signs.

Mycelium mushroom.

Dichotomously branching mycelium consists of many nuclear cells. The diameter of the hyphae varies from 2.5 to 16.5 microns. Sporangia are oblong, multi-spore, conidia fusiform. Dimensions of askopor; length 20.2- 26.7 microns, diameter 2.5-2.8 microns. On the agar medium, it forms flat, opaque (later glossy) colonies of yellow color, easily removable from the agar. The shape of the colonies is round, diameter is 8-12 mm (on the wort after 3 days of growth at). As carbon sources, glucose, fructose, raffinose, sucrose, glycerin, acetate and citrate are used. Does not absorb starch, cellulose, ethanol, inositol. Coagulates milk, slightly dilutes gelatin.

Strain synthesizes essential oil, which includes geraniol, citronellol, nerol, yi-phenylethanol

and etc.

The composition of the essential oil, which synthesizes the strain, the following, wt.%: Geraniol 65,5-80,9

Citronellol 6.0-11.4 Nerol1.8-3.4

 - Phenylethanol 9.1-20.1 The biosynthetic activity of the strain in comparison with that of the known strain is presented in the table.

Example. The strain VKM F 3009 is grown for 6 days at 28 ° C. in test tubes on canted agar soy media of the following composition,%: sucrose 1.0; Soybean meal 5,0; agar 2.0; tap water the rest, pH 6.8-7.0.

The culture is used to prepare inoculum. The sowing is carried out in a liquid nutrient medium of the following composition, wt%: glucose 0.75; peptone 0.4 sodium succinate 0.2; hydro phosphate

15

g y

thirty

g, 40

, d

20

25

potassium 0.05; Inositol O, 014; water dis-. tilirovanna the rest, pH 6.4, Cultivated on a rocking chair at 28 C for 3 days.

For the formation and accumulation of essential oil, inoculum in the amount of 5% is introduced into flasks with a liquid nutrient medium of the following composition, wt%: soybean flour 2.0; sucrose 1.0; water plumbing the rest; pH 6.5-7.0. Incubation is carried out at 28 ° C on a shaker (200-220 rpm) for 48 hours.

The essential oil is extracted with hexane. The yield of aromatic product reaches 280 mg / l. The composition of aroma-forming components, wt.%: Geraniol 75.6; citronellol 8.3; nerol 3.1; Jb-phenylzthanol 11.2, linalool 0.3; citral 0.5 and others

Eremothecium ashbyi VKM F 3009 reaches a maximum of productivity after 2 days (the rate of crop ripening is reduced by 27-50%) and the yield of essential oil is higher (1.5-3 times) compared to similar characteristics of known strains of fungi, which indicates a high biosynthetic activity of the proposed strain. At the same time, the process of obtaining an essential oil is significantly intensified, which makes it possible to increase the profitability of the industrial production of the target aromatic product. The use of the proposed strain in production makes it possible to additionally obtain up to 6.2 tons of aromatic substances.

"F o r mula invention

The strain of the fungus Eremothecium ashbyi VKM F 3009D - producing essential oil.

Strain

Nutrient Medium | Duration Efficiency,

incubation, hImg / l

CMPM F108 (known)

Mainly enzymatic

VKM F 3009D

Mainly enzymatic

VKM F 3009D

Soyeva

Tracks 57.5

70.5-136.0 155.1-190.3 138.8-141.2 142.5-160.7 201.4-280.1 170.1-205.9

Claims (1)

Claim The strain of the fungus Eremothecium ashbyi VKM F 3009D is an essential oil producer. Strain Culture medium Duration of incubation, h Productivity, mg / L TsMPM F108 (known) Basic fermentation 42 Traces 66 57.5 VKM F 3009D The main enzyme is 42 70.5-136.0 national 48 155.1-190.3 66 138.8-141.2 VKM F 3009D Soybean 42 142.5-160.7 48 201.4-280.1 66 170.1-205.9