SU1442551A1 - System for automatic control of cyclic process of continuous cultivation of microorganisms - Google Patents

Abstract

The invention relates to a control system for biotechnological processes and can be used in enterprises of the microbiological industry, using a continuous process for the microbiological synthesis of a target product. The aim of the invention is to reduce the consumption of the supplied substrate. The system contains a contour for controlling the fractional supply of the substrate and the technical means for determining the amount of oxygen consumed for the microbiological synthesis of the target product. By the amount of oxygen consumed, in advance of a known amount of consumed substrate, the efficiency of the process is judged. Substrate delivery to the fermenter is carried out in discrete doses, while in real-time, an extreme regulator is used to search for the maximum process efficiency by controlling the frequency of the doses given. Due to the synchronization of the process of microbial biosynthesis by the fractional feeding of the substrate, the substrate is more rationally consumed and the economy is increased {the first time of the process. 1 il. c (O (L

Description

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The invention relates to the microbiological industry, in particular, to automatic control systems for the process of continuous cultivation of microorganisms, and can be used in the production of fodder yeast.

The aim of the invention is to reduce the consumption of the supplied substrate.

With the continuous cultivation of microorganisms, the fractional introduction of the substrate allows synchronizing the biosynthesis process and more efficient use of the substrate, which increases the efficiency of the process.

The drawing shows a block diagram of a system for automatic control of a cyclic process of continuous cultivation of microorganisms (only the contour of controlling the fractional supply of substrate to the fermenter is shown and the contours of regulating the supply of nutrient salts, temperature, pH and pO, culture medium are not shown).

The system consists of a fermenter 1, a circuit for controlling the fractional supply of the substrate, which includes a sensor 2 for the flow rate of the supplied substrate connected through the integrator 3 for the flow rate of the feed dose, and a unit 4 for comparison with the actuator 5 installed on the substrate supply line to the fermenter and the unit 6 for the substrate dose connected to the unit 4 comparison, serially connected sensor-7 oxygen consumption, an additional integrator 8, extreme controller 9, unit 10 comparison and block 11 synchronization, while the sensor 7 with orosti oxygen intake is connected to the input of the comparator 10, whose output is connected to an actuator 5, and the output of the synchronization unit 11 is connected to a further integrator 8.

The system works as follows.

The signal of the comparator unit 10 opens the actuator mechanism 5 installed on the substrate supply line in the fermenter 1, and the substrate dose at a predetermined rate is supplied to the fermenter 1. The flow rate of the supplied dose is measured by sensor 2j, the signal of which is fed to the integrator 3 flow. The signal from the output of the integrator 3, proportional to the number

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the substrate feed, is supplied, to comparison unit 4, in which it is compared with the signal of the substrate dose setting device 6. When the input signals are equal, the comparison unit 4 generates a control signal by which the actuator 5 is closed and the integrator 3 is reset. The dose of the supplied substrate is set by the setting device 6 and is equal to the amount of the substrate that is consumed by microorganisms during single generation during steady state optimal cultivation conditions.

Sensor 7 measures the rate of consumption of oxygen by microorganisms. The signal from sensor 7 is fed to an additional integrator 8, in which the amount of oxygen consumed by microorganisms for a given period of time is calculated. A signal from the output of additional integrator 8, proportional to the amount of biomass increment, is fed to an extreme regulator 9, which controls the operation of unit 10 of comparison. The latter compares the signal of the current rate of oxygen consumption, which comes from sensor 7, with the signal of setting the minimum rate of oxygen consumption. Upon completion of the disposal of the applied dose of the substrate, the signal of the oxygen consumption rate by microorganisms decreases and, when both input signals are matched, the comparison unit 10 generates a control signal that is fed to the actuator 5 and to the synchronization unit 11. This signal opens the actuator 5 installed on the substrate supply line, and the delivery of a new dose begins, and the synchronization unit 11 controls the operation of the additional integrator 8 and sets the integration period. After the integration period is completed, the synchronization unit 11 generates a signal according to which the signal of the integral sum from the output of the auxiliary integrator 8 is fed to the extreme controller 9 and the integrator is reset. The extremity regulator 9 performs a search for the maximum efficiency of the process by correcting the signal given by the microorganisms and the minimum rate of oxygen consumption by the microorganisms.

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The proposed system provides a rational mode of fractional submission of the substrate, as well as by synchronizing the process of biosynthesis with fractional submission of the substrate, the substrate is more rationally consumed, and the efficiency of the process increases on average by 7.5% compared to the known one.

Claims (1)

Invention Formula ten A system for automatically controlling the cyclic process of continuous cultivation of microorganisms containing a feed control circuit for a nutrient substrate to a fermenter, including a nutrient substrate flow sensor and an actuator installed on the substrate supply line to a fermenter, a synchronization unit, a JQ integrator, and an extremum regulator differing from by that with: the purpose of reducing the flow of the supplied substrate, it is equipped with a speed sensor 0 five one . - the growth of oxygen consumption, an additional integrator, a doser of the supplied substrate and two comparison units, the inputs of the first of which are connected to an additional integrator and a doser of the supplied substrate, the outputs are connected to an actuator installed on the substrate supply line, and an additional integrator, and the speed sensor oxygen consumption, the integrator, the extreme controller, and the second comparison unit are connected in series, with the second comparison unit connected to the contractor Nome mechanism mounted on the substrate feed line and synchronously tion unit, connected to the output of the last integrator, wherein vtoroy.vyhod oxygen consumption speed sensor coupled to the second comparing unit, and a flow sensor coupled to the nutrient substrate integrator. CyScmpa / n