SU1424762A1 - Method of determining heat-resistance of plants - Google Patents

Abstract

The invention relates to biofield used in breeding work. The purpose of the invention is to determine the sensitivity of the determination of the heat resistance of a plant in laboratory conditions. A plant sample, for example, leaves, is placed in a calorimetic ampoule, in which it is found with moistened paper. The ampoule is introduced into the calorimeter at a temperature sublethal for this plant (for wheat, 45 ° C). The kinetics of heat release of the samples under study is recorded and their thermograms are compared, and the later the attack time and the less pronounced the fracture on the thermogram, in particular, the study of resistance, the higher the heat resistance of the given plant in adverse environmental conditions, and the plant can compared to the other 1 Il. be used in breeding work. The purpose of the invention is to increase the sensitivity of the determination of heat resistance of a plant under laboratory conditions. A plant sample, such as a leaf, is placed in a calorimetric ampoule in which the paper is moistened. The ampoule is introduced into the calorimeter at a temperature sublethal for this plant (for wheat 45 ° C). The kinetics of heat release of the studied samples is recorded and their thermograms are compared, and the later the time comes and the less pronounced the break in the thermogram is, the higher is the heat resistance of this plant compared to the others. 1 il. (L

Description

The invention relates to the biological sciences that study plant resistance to adverse environmental conditions, and can be used in selective work for the purpose of early diagnosis of heat resistance of plants, in plant breeding, when conducting biological KJtx research.

The purpose of the invention is to increase the sensitivity of determining the heat resistance of a plant organism, as a whole, in laboratory conditions.

The method is carried out as follows.

The plant organism is placed in a calorimetric ampoule with a moisture carrier in it, then the ampoule is introduced into the calorimeter at a temperature that is plant-specific for the plant, the heat release rate of the test samples is measured and their thermograms are compared, and later on the thermogram is less pronounced and less pronounced. the higher the heat resistance of this plant compared to the others.

The drawing shows the thermograms of the wheat wheat plants of the two ikotipov. notoriously different in heat resistance.

L1 1 is marked thermogram of heat-resistant (JS) varieties of wheat Saratovsk 29, Lsh-2 2 - non-heat-resistant (NLS) varieties Moskovsk 35 at sublethal temperature 45 C. Control (line K) - thermogram obtained at 25 ° C. The procedure for obtaining thermograms is carried out in accordance with known examples.

In preparation for testing for heat resistance, plants were grown under strictly identical conditions. The course of the determination is reduced to the following:

Wheat leaves (200-300 mg) are placed in a calorimetric ampoule containing wet paper.

 to prevent the plants from drying out. The ampoule is introduced into the calorimeter at a given sublethal temperature (for a 45 ° C) and recorded the heat generation rate. At the optimum temperature, the heat release rate remains unchanged during the entire time of the experiment (line K). The decrease in heat release in NLS plants (line 2) at sublethal temperature occurs significantly

faster, and the angle of fracture on the thermogram is larger compared to the LS plants (line 1).

20 f o rm a l l invention

Method for determining heat resistance of plants, including the preparation of a plant sample from plants of cp25 Himaet ix varieties or ecotypes grown under identical conditions, placing the sample in a heat chamber, measuring the temperature index, the value of which is heat resistant

30 of the plant sample, which is distinguished by the fact that, in order to increase the sensitivity of determining the heat resistance of the plant sample, as a whole, in laboratory conditions,

, with a plant sample placed in

a calorimetric ampoule with a moisture carrier in it, then the ampoule is introduced into the calorimeter at a temperature sublethal for the plant,

Q measures the heat generation rate of the samples under study and compare their thermograms, and the later the time comes and the less pronounced the break in the thermogram is, the higher the heat resistance of the plant compared to the others.

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Claims (1)

Claim A method for determining the heat resistance of j plants, including the preparation of a plant sample from plants of comparable varieties or ecotypes grown under identical conditions, placing the sample in a heat chamber, measuring a temperature indicator, the value of which is used to judge the heat resistance of a plant sample, rain and I mean that, in order to increase the sensitivity of determining the heat resistance of a plant sample as a whole, in laboratory conditions, the plant sample is placed in a calorimetric ampoule with the moisture in it carrier, then the ampoule is introduced into the calorimeter at a sublethal temperature for a given plant, the heat release rate of the studied samples is measured and their thermograms are compared, and the later the kink on the thermogram occurs and the less pronounced, the higher the heat resistance of this plant compared to the others. The residence time of the plant sample b calorimetric koi ampoule, h