SU1399681A1 - Method of investigating the serum system of human blood - Google Patents
Method of investigating the serum system of human blood Download PDFInfo
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- SU1399681A1 SU1399681A1 SU853896533A SU3896533A SU1399681A1 SU 1399681 A1 SU1399681 A1 SU 1399681A1 SU 853896533 A SU853896533 A SU 853896533A SU 3896533 A SU3896533 A SU 3896533A SU 1399681 A1 SU1399681 A1 SU 1399681A1
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Abstract
Изобретение относитс к медицине , точнее к лабораторной диагностике . Цель изобретени - упрощение спо соба и повышение его информативности. Дл этого сьшоротку крови человека или животного нанос т капеЛьно (10- 30 капель 04-5 мм) на изотропную подложку (напр, чашка Петри) с фильтровальной бумагой и помещают в электрический суховоздушный шкаф при 37 С и обычном атмосферном давлении. Сушат 3-4 ч до полного удалени влаги. Полученный препарат микроскопируют в проход щем свете при разных увеличени х: например, объектив 3,2-5 (окул р ), соответственно 8-7, 10-7, 20 7. Метод позвол ет изучать бтруктуры ксерогел сывороточных систем крови человека и животных, исследовать процесс кристаллизации собственных солей сыворотки. (ЛThis invention relates to medicine, more specifically to laboratory diagnostics. The purpose of the invention is to simplify the method and increase its informativeness. For this purpose, a person or animal's blood is deposited driply (10-30 drops 04-5 mm) onto an isotropic substrate (for example, a Petri dish) with filter paper and placed in an electric dry air cabinet at 37 ° C and normal atmospheric pressure. Dry 3-4 hours until moisture is completely removed. The resulting preparation is microscoped in transmitted light at different magnifications: for example, a lens of 3.2–5 (eye), respectively, 8–7, 10–7, 20–7. The method allows studying the xerogel structures of human and animal serum systems, investigate the crystallization process of whey's own salts. (L
Description
со соwith so
со сз:with cz:
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Изобретение относитс к медицине И может быть использовано в лабораторной диагностике.This invention relates to medicine and can be used in laboratory diagnostics.
Цель изобретени - упрощение спо- соба и повьшгение его информативности за,счет возможности оценки структурно-пространственной ориентации компонентов сьшоротки.The purpose of the invention is to simplify the method and improve its informativity, due to the possibility of assessing the structural and spatial orientation of the short components.
Способ осуществл ют следующим об- разом.The method is carried out as follows.
Сыворотку крови человека или животного нанос т в виде капель от 10 |До 30 на изотропную подложку (например , чашка Петри, предметное стекло) |И помещают в электрический суховоз- |Душный шкаф при обычном атмосферном ;давлении и при + 37 С. Сыворотку на- |нос т пипеткой, диаметр капель 4-5 мм |Изотропна подложка, промыта и npoc |шенна , датируетс замшей или фильт- |ровальной бумагой. Сушка происходит на прот жении 3-4 ч до полного удале . |ни влаги, полученный препарат мик- |роскопируют в проход щем свете при ;разных увеличени х: например, объектив 3,2-5 (окул р), соответственно 8-7, 10-7, 20-7.Human or animal serum is applied in the form of drops from 10 | to 30 on an isotropic substrate (for example, a Petri dish, glass slide) | And placed in an electric dry-carrier | | A stuffy cabinet at normal atmospheric pressure and at + 37 ° C. Serum on - | worn with a pipette, droplet diameter 4-5 mm | Isotropic substrate, washed and npoc | dated, dated by chamois or filter paper. Drying takes place over 3-4 hours to complete distance. No moisture, the resulting preparation is microscopically copied in transmitted light at different magnifications: for example, a lens of 3.2-5 (eye), respectively, 8-7, 10-7, 20-7.
I Метод позвол ет изучать структуры ксерогел сывороточных систем крови человека и животных, а также исслё- 1довать процесс кристаллизации-собст- jBeHHbiX солей сыворотки, тип которой I обусловлен структурно-пространствен- ной ориентацией белков самой системыI The method allows studying the xerogel structures of the serum systems of human and animal blood, as well as studying the crystallization process — the serum salts jBeHHbiX, whose type I is due to the structural-spatial orientation of the proteins of the system itself.
Q Q
j Q 5 j Q 5
00
Сдвиги в физико-химических свойствах сыворотки крови, обусловленные различными воздействи ми внешней и внутренней среды, сопровождаютс и изменением архитектоники ксерогел . Так как последний представл ет собой биологическую модель дл наблюдени в услови х in vitro, то возможные моди- .фикационные изменени в структуре ксерогелеграммы могут определ ть ее как шздикатор различных взаимодействий сывороточных белков с метаболитами . Сол ми и т.д.Shifts in the physicochemical properties of blood serum, caused by various influences of the external and internal environment, are also accompanied by a change in the xerogel architecture. Since the latter is a biological model for observation in vitro, possible modification changes in the structure of the xerohelegram can determine it as a diverter of various interactions of serum proteins with metabolites. Sol mi etc.
Способ позвол ет одновременно наблюдать за неорганической компонентой сывороточной систеьы крови (процесс кристаллизации солей сыворотки) и органической (сухой гель, матрица, образованна белковой компонентой сыворотки).The method makes it possible to simultaneously monitor the inorganic component of the blood serum system (the crystallization process of serum salts) and the organic one (dry gel, matrix, formed by the protein component of the serum).
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU853896533A SU1399681A1 (en) | 1985-04-03 | 1985-04-03 | Method of investigating the serum system of human blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU853896533A SU1399681A1 (en) | 1985-04-03 | 1985-04-03 | Method of investigating the serum system of human blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1399681A1 true SU1399681A1 (en) | 1988-05-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU853896533A SU1399681A1 (en) | 1985-04-03 | 1985-04-03 | Method of investigating the serum system of human blood |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1399681A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5851790A (en) * | 1995-08-22 | 1998-12-22 | Venturedyne, Ltd. | Method for drying cells using cytogenetic chamber |
-
1985
- 1985-04-03 SU SU853896533A patent/SU1399681A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Кристаллографический метод ис- . следовани биологических субстратов: Методические рекомендации. М., 1981. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5851790A (en) * | 1995-08-22 | 1998-12-22 | Venturedyne, Ltd. | Method for drying cells using cytogenetic chamber |
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