SU1395255A1 - Composition for seed treatment - Google Patents

Abstract

The invention relates to chemical plant protection products containing organic compounds including metal atoms. The purpose of the invention is to increase the effectiveness of the pickling agent. Tributyltin nitrate is used as a seed dressing agent. The compound was tested on fungal test organisms and barley seeds. The degree of seed affection of barley is halved, and the number of normally sprouted seeds is higher than in the control. 4 tab. with

Description

s / o; o

R

ate

The invention relates to chemical plant protection products containing organic compounds containing metal atoms and can be used for seed dressing.

The use of bis (tributyltin) oxide as a means for seed dressing is known.

However, bis (tributyltin) oxide. is highly toxic to warm-blooded animals.

LD

50

194 mg / kg.

The purpose of the invention is to increase the efficiency of the action of the etching agent I.

I The goal is achieved by using tributyltin nitrate.

1 (C4H,) jiSnNO, as a means

I for seed dressing,

; Tributyl nitrate was synthesized

 designed to study structural properties

: And the reactivity of tin-organic nitrates. Biological

Its activity is not described. .

To evaluate the positive effect, the proposed compound was tested on the fungal test organisms Asperglllus iHelfuinthosporium, Frichothecium, Fusa rium, which infect the soil and plants.

Example 1. Disks with a diameter of 10 mm prepared from paper, impregnated with solutions of the compounds to be studied: For imbibition, use i400 μg solution of the active substance in 1 ml of dimethyl sulfoxide. test suspension of organic matter (500 thousand, reproductive bodies per ml), the samples are incubated with 24 ° C for 14 days, the effectiveness of the compounds is determined by the value of

the brakes that form around the disc

The test results are given in Table 1,

at

Table 1 The results of the initial tests of the biological activity of tributyltin nitrate (growth inhibition zone in mm)

Test organisms, biologically active SAS I2E

13, 0

14.0

15.5 19.5 23.0 17.0 29.5 32.5

 As can be seen from the table. 1, tributyltin nitrate exhibits fungicidal activity comparable to granosan.

To determine the effectiveness of the proposed compound against fungi that infect the soil and plants, it is tested in various dilutions in comparison with granozan TMTD,

B quality control using the solvent and the medium of čapek,

In a nutrient medium (Chapek's medium), dilutions of the proposed agent are obtained, corresponding to 200,100, 50,25,12,5,6,75 µg / ml of active ingredient. Cultures of test organisms are plated onto the surface of a solid medium in a Petri dish using the plaque method. Samples are incubated at 24 ° C for 14–21 days. The efficacy of the compound is determined by limiting dilution, which retards the growth of the test cultures. In parallel, the effect of granosan, TMTD and dimethyl sulfoxide is tested.

The results are presented in table. 2,

 Table2 Assessment of the effectiveness of tributyl tin nitrate on test organisms

Test organisms

Dilution of matter, µg / ml, with a column diameter, mm

100

200

50

25

12.5

Growth is absent, depressed

50

25

12.5 6.75

7est organisms

Granozan:

Aspergillus Helminthosporium Frichotheciura Fusarium With Fusarium Oxyc

TMTD:

Aspergillus

Heltninthosporium Frichothecium Fusarium C

Fusarium Oxyc Control

Solvent (dimethyl sulfoxide)

Aspergillus

Heliainthosporium frichotheci am

Fusarium C Fusarium Dxyc

Control

Growth of crops on Wed Aspergillus

Helminthosporium Frichothecium Fusarium C Fusarium Oxyc

As can be seen from the table. 2, efficiency Example 2. Naturally, tributyltin zara-nitrate is better than 55 harvested barley seeds (100 pcs.) In TMTD and is the same as that of granozan. The aqueous emulsion is treated for 1 hour. The effectiveness of the proposed by sium of tributyl tin nitrate, containing seed disinfectants, was carried out with 6.25 µg / mp active substance, also on barley seeds, then, then spread out on sterile

Continuation of table 2

Dilution of the substance, µg / ml, with a column diameter, mm

200

G1H1;

100 50 25 I I2j5 6.75

Growth is absent, depressed

8.7

45.0 28.0 36.0 45.0 43.0

filter paper and place; germinate in a thermostat at 29 ° C, carried out after 7 days. At the same time, both general infection and the effect of the drug on the normal germination of seeds are taken into account. As a contact13952556

roll use untreated seeds, kept in sterile water in

for 1 hour

I

The results are presented in table. 3 and 4.

Table3

Evaluation of the effectiveness of tributyl tin nitrate on barley seeds

Control (Alteinarim exchange without treatment) Fusariumi

Seeds are treated with Alteruarim botans with nitratetributolol (6.75 µg / mp). The seeds are treated with granozan (6.75 µg / ml)

Fusarlum

: As can be seen from the table. 3, the degree of infection of seeds is reduced by a factor of 2: in this case, the number of normal H (| germinated seeds, higher than in the control).

Table4

I The effect of treatment with tribut-: tilolov nitrate on the growth of healthy L seed and barley (lab. Research,) n 100 seed

on, aged in sterile

for 1 hour

I

Results are presented in and 4.

37.3 77.0

7.0

16.0 82.0 4.0

10.0 90.0 0.0

five

0

five

0

five

As can be seen from the table. 4, in healthy seeds treated with tributyl tin nitrate, there is a 100% germination rate. Tributyltin nitrate in concentrations of 6.25 µg / ml, 3.13 µg / ml, with a pronounced biocidal effect, which, 6.25 µg / ml was also used for dressing infected seeds during field trials, does not have phytotoxicity and for healthy sem n

Unlike bis (tributyltin) oxide, when treating with tributyl tin nitrate: tributyl tin nitrate in an aqueous medium and the soil loses toxicity in the first 5 days; toxic degradation products do not accumulate; Nitrogen entering the soil after hydrolysis of tributyltin nitrate may have a positive effect on the plant, is a source of nutrition.

Thus, tributyltin nitrate has significant advantages compared to bis (tributylol) OXIDOM less toxic to warm-blooded animals; turn up

7est organisms

Granozan:

Aspergillus Helminthosporium Frichothecium Fusarium With Fusarium Ohusus

TMTD:

Aspergillus

Helminthosporium Frichothecium Fusarium C

Fusarium Ohus Control

Solvent (dimethyl sulfoxide)

Aspergillus

Helrainthosporium frichothecium

Fusarium With Ousus Fusarium

Control

Growth of crops on Wed Aspergillus

Helminthosporium Frichothecium Fusarium With Fusarium Ohusus

As can be seen from the table. 2, the efficiency of tributyl tin nitrate is better than that of 55 TMTD and is the same as that of granozan.

An evaluation of the effectiveness of the proposed seed treatment was also carried out on barley seeds.

Continuation of table 2

Dilution of the substance µg / ml, with the diameter of the column, mm

200 U100 TZO T 25 I I 6.75

No growth, depressed

8.7

45.0 28.0 36.0 45.0 43.0

Example 2 Naturally infected barley seeds (100 pcs.) Are treated with an aqueous emulsion of tributyl tin nitrate containing 6.25 µg / mp active ingredient for 1 hour, then spread out on sterile

fNltrvvalny paper and placed: P1 grow in a thermostat at 29 ° C. ACCOUNT is carried out after 7 days. At the same time, both general infection and the effect of the drug on the normal germination of seeds are eaten. As a contact table

Evaluation of the effectiveness of tributyl tin nitrate on barley seeds

Control (Alteinarim exchange without treatment) Fusariuffi

Seeds obra- Alteruarim botany nitratetributi- Fusarium lolovom (6.75 µg / ml) Seeds treated with granozan (6.75 µg / ml)

As can be seen from the table. 5, the degree of contamination of seeds is reduced by a factor of 2, while the number of normal sprouted seeds is higher than in control. ; Table4

Effect of treatment with tribut-: tilolov nitrate on the growth of healthy seeds of wheat (lab. Research,) n 100 seeds

roll use untreated seeds, kept in sterile water in

for 1 hour

one

The results are presented in table. 3 and 4.

37.3 77.0

7.0

16.0 82.0 4.0

10.0 90.0 0.0

As can be seen from the table. 4, in healthy seeds treated with nitrate

35 tributyltin observed 100% germination. Tributyltin nitrate in concentrations of 6.25 µg / ml, 3.13 µg / ml, with a pronounced biocidal effect, which, 6.25 µg / ml also

40 were used for seed treatment of infected seeds during field trials, and are not phytotoxic for healthy seeds.

Unlike bis (tributyltin) ox45, when treating with tributyltin nitrate: tributyltin nitrate in water and soil loses toxicity in the first 5 days; current-decay products do not accumulate;

The 50 nitrogen entering the soil after hydrolysis of tributyl tin nitrate can have a positive effect on the plant, is a source of nutrition. Thus, tributylol ovate nitrate has significant advantages over bis (tributylol) oxide; less toxic to warm-blooded animals; all 713952558

efficiency; low standards to expand the arsenal of means of transport; decomposes rapidly in soil and acts on living organisms as well as hydrosphere; There is no contamination. Replacing the environmental pollutants, the remote control of mercury compounds in agriculture. The proposed preparation opens up.

wide prospects for use in the ka-formula of the invention as an effective means of protecting agricultural crops. Use of tributyl tin nitrate

The use of tributyl tin nitrate as a means for dressing as seed disinfectants allowed seed.

Claims (1)

Claim The use of tributyl tin nitrate as a seed dressing agent.