SU1367931A1 - Method of preserving tooth rudiment - Google Patents

Method of preserving tooth rudiment Download PDF

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Publication number
SU1367931A1
SU1367931A1 SU864070337A SU4070337A SU1367931A1 SU 1367931 A1 SU1367931 A1 SU 1367931A1 SU 864070337 A SU864070337 A SU 864070337A SU 4070337 A SU4070337 A SU 4070337A SU 1367931 A1 SU1367931 A1 SU 1367931A1
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SU
USSR - Soviet Union
Prior art keywords
rudiment
honey
graft
preserving
dental
Prior art date
Application number
SU864070337A
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Russian (ru)
Inventor
Радмир Анварович Хасанов
Генадий Ефимович Драновский
Наиль Ибрагимович Хамматов
Original Assignee
Башкирский государственный медицинский институт им.ХУ-летия ВЛКСМ
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Priority to SU864070337A priority Critical patent/SU1367931A1/en
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Publication of SU1367931A1 publication Critical patent/SU1367931A1/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Description

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СОWITH

Изобретение относитс  к области медицины,а именно хирургической стоматологии .This invention relates to the field of medicine, namely dental surgery.

Целью изобретени   вл етс  сохра- нение жизнеспособности трансплантата .The aim of the invention is to preserve the viability of the graft.

Способ осуществл ют следующим образом .The method is carried out as follows.

Зубной трансплантат - зачаток после забора от донора тщательно промывают в физиологическом растворе. Промьшание в физиологическом растворе необходимо дл  удалени  с трансплантата остатков крови. Затем транс плантат помещают во флакон с пчелиным медом, где он хранитс  в течение 48-72 ч. По истечении 48-72 ч в мед добавл ют глицерин при соотношении глицерин - мед 3:1 и затем флакон с зубным трансплантатом помещают в холодильник и хран т при 5°СDental graft - the bud after collection from the donor is thoroughly washed in saline. A saline solution is necessary to remove blood residues from the graft. Then the trans plantation is placed in a bottle of honey, where it is stored for 48-72 hours. After 48-72 hours, glycerin is added to the honey at a glycerin-honey ratio of 3: 1 and then the bottle with a dental graft is placed in a refrigerator and stored t at 5 ° C

Пример. Берут зубной трансплантат двухмес чных щен т, тщательно его промывают в физиологическом растворе и помещают в пчелиный мед, где его хран т в течение 50 ч. Специальными бактериологическими методами исследовани  устанавливают стерильность зубных трансплантатов без применени  каких-либо антибиотиков . После этого в мед добавл ют глицерин при соотношении глицерин - мед 311. Через 1,2,6,12,18,24 мес цев зубные трансплантаты подвергают гистологическим,гистохимическим и электронно-микроскопическим исследовани м с целью определени  жизнеспособности трансплантата. При этом установлено, что в течение 18 мес цев на гистологических препаратах консервированных зубных трансплантатов отчетливо определ ютс   дра аме- лобластов, одонтобластов и пульпо- цитов. На электроннограммах видны митохондрии,где сохранилась двухслой ность наружной их мембраны и хорошо определ ютс   дерна  и клеточна  оболочка. Сохранение этих клеточных структур при консервации органов укзывает на жизнеспособность клеток. Жизнеспособность трансплантата при консервации предлагаемым способо сохран етс  18 мес по сравнению с известным (30-60 дней).Example. A two-month-old dental graft is taken, it is thoroughly washed in physiological saline and placed in bee honey, where it is stored for 50 hours. Special bacteriological methods of examination establish the sterility of dental grafts without the use of any antibiotics. After that, glycerol is added to the honey at a glycerin to honey ratio of 311. After 1,2,6,12,18,24 months, dental grafts are subjected to histological, histochemical, and electron microscopic studies to determine the viability of the graft. It was found that, within 18 months, the histologic specimens of preserved dental grafts clearly define the nuclei of ameloblasts, odontoblasts, and pulpocytes. On the electron diffraction patterns, mitochondria are visible, where the bilayer of their outer membrane is preserved and the sod and cell membrane are well defined. Preservation of these cellular structures during organ preservation indicates the viability of the cells. The viability of the graft during the preservation of the proposed method is maintained for 18 months compared with the known (30-60 days).

Claims (1)

Формула изобретениInvention Formula Способ консервации зубного зачатка путем забора его от донора и промывани  в физиологическом растворе, отличающийс  тем,что, с целью сохранени  жизнеспособности, трансплантат после промывани  помещают в пчелиный мед на 48-72 ч, а затем добавл ют глицерин при соотношении глицерин-мед равном 3:1.A method of preserving a tooth germ by collecting it from a donor and washing it in a physiological solution, characterized in that, in order to maintain viability, the transplant after washing is placed in bee honey for 48-72 hours, and then glycerol is added at a glycerin-honey ratio of 3 :one.
SU864070337A 1986-04-03 1986-04-03 Method of preserving tooth rudiment SU1367931A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU864070337A SU1367931A1 (en) 1986-04-03 1986-04-03 Method of preserving tooth rudiment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU864070337A SU1367931A1 (en) 1986-04-03 1986-04-03 Method of preserving tooth rudiment

Publications (1)

Publication Number Publication Date
SU1367931A1 true SU1367931A1 (en) 1988-01-23

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SU864070337A SU1367931A1 (en) 1986-04-03 1986-04-03 Method of preserving tooth rudiment

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SU (1) SU1367931A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019098833A1 (en) * 2017-11-15 2019-05-23 Triticum Exploitatie B.V. The use of a medical grade honey based ingredient for the preparation of a composition for preserving human and/or animal tissue

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019098833A1 (en) * 2017-11-15 2019-05-23 Triticum Exploitatie B.V. The use of a medical grade honey based ingredient for the preparation of a composition for preserving human and/or animal tissue
US11160272B2 (en) 2017-11-15 2021-11-02 Triticum Exploitatie B.V. Methods for preserving tissue with medical grade honey

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