SU1354093A1 - Separating chamber for fan-type electrophoresis - Google Patents

Abstract

The invention relates to a medical laboratory technique, namely to devices for the preparative electrophoretic separation of high molecular weight biological substances, suspensions of microorganisms and tissue homogenates. The aim of the invention is to increase the resolution and performance of the camera. The goal is achieved by the fact that the fraction collector is made in the form of slots located on the front wall at the height of the separation chamber in the vertical plane. The fraction collector simultaneously with the selection produces the concentration of the separated fractions. The length of the selectors is equal to the width of the front wall of the chamber. The inlets and outlets of the selectors are located on the end surface of the separation chamber. 2 Il. I (L OO sd about from 00

Description

one

The invention relates to a medical laboratory technique, namely, devices for the preparative electrophoretic separation of high molecular weight biologic substances of suspensions of microorganisms and tissue homogenates, and is intended for preparative separation with simultaneous concentration of high molecular weight biological substances and cell suspensions.

The purpose of the invention is to increase the resolution and performance of the camera.

Figure 1 shows a separation chamber for fan electrophoresis; figure 2 - collector fractions.

The chamber contains electrophoretic. Zone 1, electrode capacitances 2 with electrodes separated from the chamber by a semi-permeable membrane 3, injector 4, a collector of 5 fractions located on the front wall of the chamber, and

sides of the faction collector.

The collector 5 of the fractions is made in the form of a unit containing slot-hole capacitances-accumulators 7, separated from the electrode tank 2 and one from the other by profiled semi-permeable membranes 3 (for example, ion-exchange membranes or dialysis cellophane), located on the lateral sides of the collectors 6, located from the lateral 25 and great performance.

The location of the fraction collection manifold on the front wall of the chamber allows for a quick and clean preparation to be obtained quickly and easily. The use of circulation of the working buffer solution reduces its consumption. The separation chamber for fan electrophoresis can be used for the preparative preparation of storage tanks 7. From the electro-35 highly purified biological ve- roetic zone 1, a collector of fractions, cell cultures and microorganisms is separated by large-pore membranes for medical purposes and for - Noah 8 (for example, from a fluoroplastic native household, canvases).

The separation chamber for fan 40F formula of the invention electrophoresis works as follows: separation chamber for fan-fold. -.

Buffer solution 9 is fed into the electrophoretic zone 1 by a laminar flow. Injector 4, a mixture of particles is fed into the separation zone by an uninterrupted flow, and the voltage from the current source is applied to the electrodes. In the electrophoretic zone 1 is set

electrophoresis, containing an injector, electrode zones separated from the electrophoretic zone by a semipacted membrane, and a fraction collector, which, in order to increase the resolution and performance of the chamber,

homogeneous electric field directed across the buffer stream. Under the action of an electric field, a mixture of particles is divided into a number of fractions differing in electrophoretic mobility. Divided fractions 10 income m to the front wall with a collector of 5 fractions and popada10

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They are fed into slotted storage tanks 7. Of the latter, through the sampling unit 6, the fraction enters a special tank. The degree of concentration of the substance in the storage tank 7 depends on the ratio of the flow rate of the liquid in the electrophoretic zone 1 and the sampling rate of the separated substances through the selectors 6. The flow of the working buffer solution 9 does not fall into the storage tank 7, the entire separation zone passes, the buffer is discharged into tank with waste liquid. In that

In this case, the working buffer can be reused, which reduces its consumption.

To preserve the laminar flow in the separation chamber, it is necessary to supply the mixture with the speed and direction that coincides with the speed and direction of the buffer at the injection site.

The chamber has the simplicity of the connotation of the formula of the invention. Separation chamber for fan-

electrophoresis, containing an injector, electrode zones separated from the electrophoretic zone by a semipacted membrane, and a fraction collector, which, in order to increase the resolution and performance of the chamber,

50 is placed in a vertical plane along the height of the front wall of the separation chamber, the length of each sampler being equal to the width of the front wall, and each sampling unit is provided with an inlet 55 and outlet openings located on the end surface of the separation chamber.

FIG. one

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Compiled by And, Rogal Editor I.Gorna Tehred L.Serdyukova

Order 5687/39 Circulation 776 Subscription

VNIIPI USSR State Committee

for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab. 4/5

Production and printing company, Uzhgorod, Projecto st., 4

Proofreader O. Kravtsov

Claims (1)

40 Claims of the invention A separation chamber for fan electrophoresis comprising an injector, electrode zones separated from the electrophoretic zone by a semipermeable membrane, and a fraction collector, characterized in that, in order to increase the resolution and productivity of the chamber, the fraction collector is arranged in a vertical plane along the height of the front wall of the separation chamber, and the length of each sampler is equal to the width of the front wall ·, and each sampler is equipped with inlet and outlet GOVERNMENTAL on the end surface of the separation chamber.