SU1004852A1 - Device for electrophoretic particle separation - Google Patents

Description

The invention relates to a technique for separating particles, differing in electrophoretic mobility, and can be used to separate or biological mixtures.

A device for electrophoretic separation of particles is known, comprising a vertical rectangular separation chamber, on the sides of which electrodes, an injector, a collector and a device for supplying: buffer fluid fl

However, this device has a low separation performance due to the lateral distortion of the prlos. of fractions caused by the liquid flow and the p6osm6 eict fluid flow in a flat canoe.

The closest to the invention is an offset electrophoresis device in which electrolyte additives are introduced into the carrier buffer to compensate for sickle-shaped distortions, which results in a temperature gradient preventing the distortion of fractions 2 in the gap of the separation chamber.

However, in the known device electrolytic additives change the electrophoretic mobility of the particles.

cause coagulation of the separated particles, and the establishment of the temperature, gradient deteriorates the stability of the carrier fluid, which does not allow to increase the performance of the device.

The purpose of the invention is to increase the performance of the device.

The goal is reached

10 by the fact that in the device containing an air-assisted rectangular separation chamber, on the sides of which electrodes, an injector, a collector and a device for supplying buffer fluid are located

15 bones, channels are made in the walls of the separation chamber, connected through a series of openings to the separation chamber, and the device is additionally equipped with an EF of buffer fluid connected to the channels.

FIG. 1 shows the proposed device, front view; Fig, 2 is the same section. - ,. The device contains ploskonapal

25 is a vertical separation chamber, 1c, a base 2, which is bounded by walls 3 and 4 of a dielectric material. The chamber includes two parallel electrode compartments 5, in

Claims (2)

30 of which electrodes 6 are located, separated from the separation chamber by membranes 7. Buffer channels 8 are made in the separation chamber housing, which are connected through gap 2 of separation chamber 1 and through flow regulator 10 to transverse flow unit buffer fluid 11. The chamber also contains a fitting 12 for supplying a laminar fluid flow, an injector 13 for introducing the mixture to be separated, and a manifold 14. The gap of the separation chamber is established with a gasket .15. The device works as follows. In the buffer liquid flowing through the separation chamber chamber through the injector 13, a separable mixture is introduced. When connecting electrodes b, under the action of the field, the mixture separates c into a number of fractions consisting of particles equal to electrophoretic mobility. Separated fractions through collector 14 are removed from the device. Under the action of an electric field and forces in a particle, a strip of particles acquires a sickle-shaped form, and charged particles that are located on different. distances from the chamber walls move at different speeds and deviate at different angles in the electric field. Through a series of holes 9, a cross-flow of buffer fluid is supplied to the separator 1 from the buffer channels 8 by means of the fluid supply unit 11. Using the flow adjuster 10, the transverse flow rate is set at which maximum compensation of sickle-shaped distortion of the particle flow is observed. The application of the proposed device allows to increase the separation efficiency by eliminating the sickle-shaped distortion of the jet of particles being separated, and, moreover, to increase the electric field intensity in the separation chamber due to the use of a separation chamber of smaller thickness, as well as to increase the performance of the device due to fuller utilization the volume of the separation chamber. Apparatus of the Invention A device for electrophoretic separation of particles, comprising a vertical, shallow rectangular separation chamber, on the sides of which an electrode is arranged), an injector, a collector and a node for supplying carrier fluid, characterized in that, in order to increase the performance of the device, in the chamber walls the separation channels are connected through a series of holes to the separation chamber, and the device is additionally equipped with a buffer fluid supply unit connected to the channels. Sources of information taken into account in the examination. 1. US patent 3412007, class 204299, 11.10.68. 2. For Germany FRG 2508844, cl. C 25 V 7/00, 11/30/76 (prototype).