SU1331890A1 - Strain of azomonas agilis bacteria for meat preservation - Google Patents

Abstract

This invention relates to microbiology and can be used to preserve meat. The aim of the invention is to obtain a new strain of bacteria that can preserve meat. The strain of aerobic free-nitrogen-fixing bacteria Azomonas agilis CMPM B-3163 is grown in whey or in a sterile manner for 2 days. When applied to the entire surface of the honey of the microbial mass of the strain, the strain of m is prevented from rotting. The strain is resistant to antibiotics, produces lactic acid - 0.53, vitamins - PP, Bf, B and amino acids. It is an antagonist in relation to putrefactive bacteria: Proteus vulgaris, Staphylococcus aureus, Pseudomonas aeruginosa. Salmonella dublin. Salmonella abortus equi, Salmonella cholerae suis, Salmonella pullorum, Aeromonas punctata. fS (L CO 00 00 CD P

Description

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This invention relates to microbiology and is an antibiotic resistant strain of aerofixed bacteria used for the preservation of meat.

The purpose of the image is to obtain a new strain of nitrogen-fixing bacteria, which directly grows on a cheap nutrient medium and accumulates biomass that can delay the rotting of meat without changing its commercial value.

The strain of the bacteria Azomonas agilis, which when accumulated on a cheap nutrient medium for two days accumulates large biomass, is not pathogenic and non-toxic, resistant to streptomycin. (10,000 μg / ml), can prevent rotting of meat from

The strain of bacteria Azomonas agilis is extracted from the soil of Alma-Ata by sowing soil samples on Ashby's environment, followed by the isolation of pure cultures. Notifying free-living aerobic bacteria for the synthesis of vitamins, amino acids, lactic acid, which have antagonistic properties against putrefactive and suppurative bacteria.

The selected bacterial strain Azonomas agilis, which meets the indicated requirements, was assigned the number 8. The strain was deposited in the central museum of industrial microorganisms of the VNIIGenetics Institute, number B-3163.

The strain is identified by the determinant of bacteria Bergi,

The strain Azomonas 8 (B-3163) grows on MPA, Homtinger agar, milk, whey, Ashby medium, Beyerink medium, on Bogdanov agar, on grass extract, on manure agar, on animal agar.

In all environments, colonies of 8-shaped mucous, juicy, non-pigmented, rod-shaped cells with rounded ends in the form of ovoid are polymorphic. On the Ashbie medium, the cells are small, long threads are found on the milk, and long, thick sticks on Hottinger's agar. On the MPB it causes turbidity of the environment, Gravare Abelen, grows at-pH 4.5-9.0, the optimum pH is 7-7.5. The optimal growth temperature is 20-30 ° С, it dies at 65 ° С.

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Aerobe can grow under reduced oxygen pressure. Non-symbiotically captures nitrogen

in the presence of carbon.

Gelatin does not liquefy, reduces methylene blue. Does not decompose dulcite, decomposes glucose, lactose, sucrose, mannitol, maltose to form acid and gas. Does not possess amyloletic activity, does not hemolyze erythrocytes of sheep. Kata is lasopositive.

Relation to nitrogen sources:

use ammonia, amino acids. Galatolerantin, phage-resistant, forms lactic acid on sterile skimmed milk and whey 0.531 acidity

62 ° T, pH 5.07. Forms vitamins of group B (B, B, B). Stored freeze-dried or on sterile skimmed milk, or on Ashby's medium at 37 C. Has a genetic mark, resistant to streptocycin, mycin (10,000 µg / ml).

Antagonistic properties of the strain. The contact medium was sterile skimmed milk and sterile water. Cultures were used: Proteus vulgaris Staphylococcus aureus, Pseudomonas aeruginosa. Salmonella dublin, Salmonella abortus equi. Salmonella Cholerae suis,

Salmonella pullorum, Aeromonas punctata.

To each tube was added the same amount of the studied culture (500,000,000 cells) and the same

the amount of culture of Azomonas agilis (50,000,000 cells) per milliliter of contact medium. A day later, seeding was carried out on elective nutrient media. On special environments

only a strain of Azomonas agilis was observed. Therefore, the strain Azomonas agilis reliably retards the growth of cultures of Proteus vulgaris, Staphylococcus aureus, Pseudomonas aeruginosa,

Salmonella dublin. Salmonella abortus egu i Salmonella cholerae suis, Salmonella pullorum, Aeromonas punctata.

An example of using strain for

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preparation of meat preserving agent. .

For the cultivation of shtadmma, whey whey is used, the pH of which is adjusted to 7.4 is sterilized at a pressure of 1.5 atm. For 30 minutes. The Azomonas agilis 8 strain in an amount of 500 million microbial bodies per 100 ml of medium is injected into a sterile nutrient medium. Cultivated in a thermostat at 31 ° C for 2 days. A 2 day culture of the Azomonas agilis 8 strain is a preservative, which can be dried freeze or spray, ig under normal mode.

The titer of cells in the culture fluid 5-10 cells. The amount of niacin, thiamine, riboflavin in a lyophilized preparation was 1.22 mg / g%, and lactic acid 0.33.

The drug contains the following vitamins, mg / g%:

PP (niacin) 0.9

Bj. (thiamine) 0.27 20

B (rib flavin-) 0.05 and amino acids, g / 100 g of product: Lysine0.489

Histidine, 146

Arginine 0.119 25

Asparaginova 0.302

Threonine 0.131 Serine 0.146

Glutamine 0.743

Prolin0,086 30

Glycine, 0.050

Alanya, Oh, 122

Cystin, 156

Valin 0.337

Methionine 0.335

Isoleucine Leucine 0.442

Tyrosine0,161

Phenylalanine 0.182

Tryptophan 0.052 to

Amount -3,999

The protein content is 4.5 g / 100 g. The fat content is 2.3 g / 100 g of the product.

The production of lactic acid is determined on medium with chalk. Around the lactic acid producing colonies, a zone of dissolution of spruce is formed. To quantify lactic acid, culture is done by strain 50a on sterile skimmed milk, after 2-day growth and a clot formation, the acidity of milk is determined by Turner's degrees and multiplied by, 009 (1 ml of 0.1N alkali equivalent 55 Å 0.009 g of lactic acid in the water).

The amount of vitamins and amino acids are determined by microbiological

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method (the most reliable of all existing) using indicator cultures of auxotrophic mutants for each vitamin and amino acid (yeast and E. coli). Pichia fermentans Lodder for the determination of thiamine, Saccharomyces cerevisial pantothenic acid, Dibariomyces disporus - pyridoxine, Lygosaccharomyces baillii - biotin, Saccharomyces carlsbergens - inositol, Lygo-fabospora marxiana (Hensen) - nicotine.

To determine the biosynthesis of amino acids, auxotrophic Escherichia coli strains deficient in methionine (VKM - B - 362), histidine (VKM - B - 469), tyrosine (VKM - B - 4.0), and tryptophan (VKM - B - 471) leicin (VKM - B - 827), proline (VKM - B - 830), arginine (VKM - 831), cystine and histidine (VKM - B - 835), serine and glezina (VKM - B - 836) , lysine (VKM - 363), obtained from the department of type cultures of the Institute of Microbiology of the Academy of Sciences of the USSR.

An example of using a preparation for preserving meat.

The dry culture (or culture removed from the nutrient medium) of the Azomonas agilis strain is applied to the entire surface of a fresh piece of meat weighing -500 g and a thawed piece of meat weighing 500 g, m is placed (each piece separately) in a plastic bag. and incubated at room temperature (25-30 ° C) for 45 days. Similar pieces of meat (control) are kept in cellophane bags under the same temperature conditions.

After 45 days, fresh and thawed, mso, preserved by dry and native cultures of the strain Azomonas agilis, are removed from plastic bags and examined for freshness organoleptically and bacteriologically.

Test results: when opening cellophane bags with experimental preserved catfish, no putrid odor was detected. The structure and color of the mac is completely preserved. When sowing on special nutrient media, no rotting germs were found. In all cases

S1331890

Claims (1)

The culture of the Azomonas strain was seeded. The formula of the invention is agilis. Thus, the proposed strain. The bacterial strain Azomonas agilis allows preserving the mousy without addition of UUIM B - 3163 to preserve the leaching of chemicals.