SU1312773A1 - METHOD OF OBTAINING L-FUCO-SPECIFIC LECTIN - Google Patents

METHOD OF OBTAINING L-FUCO-SPECIFIC LECTIN

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Publication number
SU1312773A1
SU1312773A1 SU3856501/14A SU3856501A SU1312773A1 SU 1312773 A1 SU1312773 A1 SU 1312773A1 SU 3856501/14 A SU3856501/14 A SU 3856501/14A SU 3856501 A SU3856501 A SU 3856501A SU 1312773 A1 SU1312773 A1 SU 1312773A1
Authority
SU
USSR - Soviet Union
Prior art keywords
obtaining
specific lectin
fuco
raw materials
ammonium sulfate
Prior art date
Application number
SU3856501/14A
Other languages
Russian (ru)
Inventor
В.Е. Никитина
Ю.В. Итальянская
Original Assignee
Институт биохимии и физиологии растений и микроорганизмов АН СССР
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Институт биохимии и физиологии растений и микроорганизмов АН СССР filed Critical Институт биохимии и физиологии растений и микроорганизмов АН СССР
Priority to SU3856501/14A priority Critical patent/SU1312773A1/en
Application granted granted Critical
Publication of SU1312773A1 publication Critical patent/SU1312773A1/en

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Abstract

Способ получения L-фукозоспецифичного лектина путем экстракции сырья буферно-солевым раствором, центрифугирования экстракта, осаждения целевого продукта сульфатом аммония и органическим растворителем, очистки диализом и сушки, отличающийся тем, что, с целью упрощения способа за счет сокращения числа операций и повышения выхода целевого продукта, в качестве источника сырья используют бактерии Azospirillum brasilense Sp. 7, экстракцию ведут при соотношении сырье : растворитель 1 : 8 - 1 : 12, осаждение осуществляют сульфатом аммония до насыщения 0,42 - 0,48, а в качестве органического растворителя используют спирт или ацетон.The method of obtaining L-fucose-specific lectin by extracting raw materials with a buffer saline solution, centrifuging the extract, precipitating the target product with ammonium sulfate and an organic solvent, cleaning with dialysis and drying, characterized in that, in order to simplify the method by reducing the number of operations and increasing the yield of the target product , Azospirillum brasilense Sp. 7, the extraction is carried out at a ratio of raw materials: solvent 1: 8 - 1: 12, the precipitation is carried out with ammonium sulfate to a saturation of 0.42 - 0.48, and alcohol or acetone is used as an organic solvent.

SU3856501/14A 1985-03-05 1985-03-05 METHOD OF OBTAINING L-FUCO-SPECIFIC LECTIN SU1312773A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU3856501/14A SU1312773A1 (en) 1985-03-05 1985-03-05 METHOD OF OBTAINING L-FUCO-SPECIFIC LECTIN

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU3856501/14A SU1312773A1 (en) 1985-03-05 1985-03-05 METHOD OF OBTAINING L-FUCO-SPECIFIC LECTIN

Publications (1)

Publication Number Publication Date
SU1312773A1 true SU1312773A1 (en) 2001-03-20

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ID=60543098

Family Applications (1)

Application Number Title Priority Date Filing Date
SU3856501/14A SU1312773A1 (en) 1985-03-05 1985-03-05 METHOD OF OBTAINING L-FUCO-SPECIFIC LECTIN

Country Status (1)

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SU (1) SU1312773A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2524425C2 (en) * 2008-07-22 2014-07-27 Й-Оил Миллс, Инц L-FUCOSE α1→6 SPECIFIC LECTIN

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2524425C2 (en) * 2008-07-22 2014-07-27 Й-Оил Миллс, Инц L-FUCOSE α1→6 SPECIFIC LECTIN

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