SU1312094A1 - Unit for growing microorganisms or cells - Google Patents

Abstract

This invention relates to the microbiological industry and can be used to produce biomass of yeast and other microorganisms. The aim of the invention is to simplify the installation design. The plant for the cultivation of microorganisms or cells contains a fermenter, in the housing 1 of which a mixing device, aerator, loading and unloading pipes are placed. The heat exchanger connected to the device for preparing the heat carrier is made in the form of a smooth wall (L 00 o with Jiai. 25 2

Description

Noah of the shirt 7, attached by its end surfaces to the body 1 of the fermenter. The housing 1. and the jacket 7 form a heat exchange cavity 8, in the lower part of which two annular partitions 9 and 10 are located one above the other, forming cavities for the heat carrier 11 and 12. The upper partition 9 is made of porous metal or cermet to disperse the liquid by gas located on its surface. The cavity 8 and the lower cavity 12 are interconnected by means of nozzles protruding above the porous annular partition 9. On the shirt 7, there are rows of horizontally arranged slit holes 14, directly above the ring

This invention relates to the microbiological industry and can be used to produce biomass of yeast and other microorganisms.

The purpose of the invention is to simplify the installation design.

FIG. 1 shows the installation, general view; in fig. 2 — node I in FIG. 1 (heat exchanger)} in FIG. 3 shows section A-A in FIG. 2 | in fig. 4 shows section B-B in FIG. 2

The plant for cultivating microorganisms or cells contains a fermenter, in the housing 1 of which a mixing device 2, an aerator 3, and loading and unloading pipes 4 are placed. The heat exchange device 5 connected to the device 6 for preparing the heat transfer medium is made in the form of a smooth-walled jacket 7 fixed with its end surfaces to the body 1 of the fermenter. The housing 1 and the jacket 7 form a heat exchange cavity 8, in the lower part of which there are two annular partitions 9 and 10 one above the other, a gap e and cavities 11 and 12 for the heat carrier. The upper partition 9 is made of porous metal or metal ceramics for gas dispersion of a liquid located on its new partition 9 of a series of holes 14 is connected by a collector 15 serving to supply and distribute the liquid over the surface of the porous ring partition 9. A device for preparing heat transfer media is made of a vortex pipe connected and pressure line and the atmosphere through the valves. In the upper part of the jacket 7, on the outside of the jacket, there is fixed a casing 20, the lower part of which has the shape of a truncated cone. On the jacket 7, directly below the junction of the lower part of the casing 20, there is a series of slotted holes 14, and inside the casing 20 with a gap relative to the jacket 7 there is an annular disk 21 dividing the casing into two cavities. 4 il.

surface. The cavity 8 and the lower cavity 12 are interconnected by means of nozzles 13 protruding above the porous annular partition 9. The shirt 7 has a series of horizontal slit holes 14. Directly above the annular partition 9, a series of holes 14 are connected by a collector 15, serving

for the supply and distribution of fluid over the surface of a porous, annular partition 9,

The device 6 for the preparation of the coolant is made of a vortex

pipes 16 connected to the pressure line through valve 17, and through valves 18 and 19 to the atmosphere. B of the upper part of the shirt 7 on the outside of the casing is fixed 20, the bottom

part of which has the shape of a truncated cone. On the shirt 7, directly below the junction of the lower part of the casing 20, there are a number of slits in the holes 14, and inside the casing 20

with a gap relative to the jacket 7, an annular disk 21 is fixed, dividing the casing into two cavities.

The casing 20 has a fitting 22, which, together with the annular disk 21 and a series of slotted openings 14, serves to drain gas from the heat exchanger 5 to the discharge line. The rows of slotted holes 14 located between the casing 20 and the collector 15 are united by a collector 23 and serve to supply coolant to the cavity 8. To fittings 24-26, a device 6 for heating coolant preparation is connected by means of gates 27 to 31,

To the fitting 32 of the collector 15, by means of a pipeline with a valve 33, the under-JO is aspirated, cooled and returned,

a container 34 is connected to the liquid; a nozzle 22 is connected by a branch pipe 35 passing through the container 34 to the atmosphere.

The installation works as follows: J5.

Depending on the need to cool or heat the liquid in the fermenter, the valves 30, 17, 18, 20, 29, 29, 29, respectively, are opened to cool the heat exchange medium to the heat exchange zone. Inevitable minor fluid losses are compensated for by liquid) from tank 34, through which the outgoing gas passes and pre-cools (heats) the fluid.

The working cycle of the fermenter is as follows.

The fermenter through pipe 4 is filled with a nutrient medium. Through the same pipe, seed is introduced into it. Air is supplied through the aerator 3 to the apparatus. The mixing of the culture liquid and the dispersion of air are carried out by the mixing device 2. The cultivation temperature T, depending on the type of the cultivated culture, can be in the range of 20-40 ° C. Therefore, before cultivation, it is possible to bring the culture liquid to the cultivation temperature, while cold or hot air is supplied to the heat exchanger. The fermentation process comes with the release of heat. When the producer of streptomycin is cultivated up to the 12th hour, there is a rapid increase in the heat flux, which goes to the console, and for hot water - to the valves 17, 31, 19. 27 - 29.

The need to obtain different intermediate gas temperatures is achieved by mixing hot and cold gases.

The main gas stream is supplied to the nipple 25 via the valve 27, the auxiliary gas flows to the nipples 24 and 26 through the valves 28 and 29, the first being designed to disperse and precool the liquid entering the porous annular partition 9 through the nipple 12, the manifold 15, the slot opening 14 from the tank 34 and the flow controller - valve 33. Dispersion is obtained by flushing the porous surface of the annular partition 9 with gas, moistened with a liquid. The liquid dispersed to a mist with a droplet diameter of about 0.1 mm and pre-cooled is picked up by the main stream passing through the nozzles 13, further cooled and introduced into the cavity 8, where auxiliary streams enter the stream from the slotted holes 14. This leads to the turbulence of the main flow and uniform heat removal throughout the flow path, as well as to the effect on the liquid film on the housing 1. All this increases the heat removal. The passage through the jacket -20 of the gas-liquid coolant loses the main part of its liquid, which through the slotted holes 14 returns to the cavity 8. The fluid falling on the inner surface of the jacket 7 and flowing down it, falling into the slotted holes, is dispersed, cooled and returned to the zone heat exchange. The part of the liquid that reaches the annular partition 9 also disperses into the heat exchange zone. Inevitable minor fluid losses are compensated for by liquid) from tank 34, through which the outgoing gas passes and pre-cools (heats) the fluid.

The working cycle of the fermenter is as follows.

The fermenter through pipe 4 is filled with a nutrient medium. Through the same pipe, seed is introduced into it. Air is supplied through the aerator 3 to the apparatus. The mixing of the culture liquid and the dispersion of air is carried out by the mixing device 2. The cultivation temperature T, depending on the type of the cultivated culture, can be in the range of 20-40 ° C. Therefore, before cultivation, it is possible to bring the culture liquid to the cultivation temperature, while cold or hot air is supplied to the heat exchanger. The fermentation process comes with the release of heat. When cultivating the producer of streptomycin until the 12th hour, there is a rapid increase in heat flux, which in the complex

The price of this segment is 25,000 g. / m h

At the same time, cold air begins to be supplied to the heat exchanging device of the fermenter, with increasing flow, for which the valves are closed and the valves 23 and 30 are opened. By the 12th hour of the process, the air flow reaches the maximum value for this process and ensures that

Peruture dispersed liquid t 17-20 0, which provides the necessary heat removal. The regulation / temperature of the fluid T is made by the valves 25 and 27. After the 12th hour of the 5th hour and up to the 80th hour, the first is fast until the 40th hour, and then the slow decrease in heat flow

before . m h

Heat collection ensured513

at a temperature of tj 30-32 C. After completion of the cultivation process, the finished product is cooled if necessary and discharged through pipe 4,

A fermenter with a heat exchanging device reduces the costs of creating equipment for thermostating by 30-50% and the total cost of all devices necessary for cultivation by 6-11%. This is made possible by a simple and cheap heat exchanging device, which makes it possible to use gas as a heat carrier much more efficiently.

Claims (1)

Invention Formula An apparatus for culturing microorganisms or cells, comprising 46 a fermenter, a heat exchanger in the form of a jacket mounted on the body of the fermenter, and a device for preparing a heat transfer fluid supplied to the jacket, characterized in that, in order to simplify its design, the device for preparing a heat transfer fluid is made in the form of a vortex tube and in a shirt the heat exchange device is fixed in the lower part one above the other by two annular partitions with the formation of cavities for the heat transfer medium, the upper partition is made of porous material, and the lower cavity is connected by nozzles with a shirt cavity, the latter having a height of rows of horizontally located slit holes for the supply of heat carrier. 1 Compiled by Y. Utkin Editor N. Rogulich Tehred A. Kravchuk Proofreader S. Cherni Order 1935/24. Circulation 500 Subscription VNIIPI USSR State Committee for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5 Production and printing company, Uzhgorod, Projecto st., 4 needles