SU129656A1 - METHOD OF OBTAINING D'-DEHYDROCORTIZON - Google Patents
METHOD OF OBTAINING D'-DEHYDROCORTIZONInfo
- Publication number
- SU129656A1 SU129656A1 SU642743A SU642743A SU129656A1 SU 129656 A1 SU129656 A1 SU 129656A1 SU 642743 A SU642743 A SU 642743A SU 642743 A SU642743 A SU 642743A SU 129656 A1 SU129656 A1 SU 129656A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- obtaining
- dehydrocortizon
- culture
- cortisone
- acetate
- Prior art date
Links
- ITRJWOMZKQRYTA-RFZYENFJSA-N Cortisone acetate Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)COC(=O)C)(O)[C@@]1(C)CC2=O ITRJWOMZKQRYTA-RFZYENFJSA-N 0.000 description 3
- 241000203720 Pimelobacter simplex Species 0.000 description 3
- XOFYZVNMUHMLCC-ZPOLXVRWSA-N Prednisone Chemical compound O=C1C=C[C@]2(C)[C@H]3C(=O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 XOFYZVNMUHMLCC-ZPOLXVRWSA-N 0.000 description 3
- 229960003290 cortisone acetate Drugs 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229960004618 prednisone Drugs 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000194107 Bacillus megaterium Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000002609 media Substances 0.000 description 2
- YMWUJEATGCHHMB-UHFFFAOYSA-N methylene dichloride Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- MFYSYFVPBJMHGN-ZPOLXVRWSA-N Cortisone Chemical compound O=C1CC[C@]2(C)[C@H]3C(=O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 MFYSYFVPBJMHGN-ZPOLXVRWSA-N 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- UHOVQNZJYSORNB-UHFFFAOYSA-N benzene Substances C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 235000005824 corn Nutrition 0.000 description 1
- 229960004544 cortisone Drugs 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000001963 growth media Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002906 microbiologic Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
Description
Изобретением вл етс способ получени преднизона из кортизон-ацетата с помощью микробиологического дегидрироваии и омылеии смесью культур Corynebacterium simpeex u Bacillus megatherium ВПИХФИ-1.The invention is a method for producing prednisone from cortisone acetate by microbiological dehydration and saponification with a mixture of cultures of Corynebacterium simpeex and Bacillus megatherium VPHFI-1.
Метод выгодно отличаетс от известных тем, что исходит из товарного кортизона и дает возможность получать преднизон в одну стадию.The method favorably differs from those known in that it comes from commercial cortisone and makes it possible to obtain prednisone in one stage.
Сиособ осун ествл етс следующим образом .The arrangement is as follows.
В ферментер помещают 8 л культуральной среды, содержащей 0,5 дрожжевого автолизата , 0,5% глюкозы и водопроводную воду, имеющую рН 7.8 liters of culture medium containing 0.5 yeast autolysate, 0.5% glucose and tap water having a pH of 7 are placed in the fermenter.
Культуру Corynebacterium simplex выращивают в матрицах на твердой агаровой среде, содержащей экстракт кукурузы н глюкозы, в течение 72 час, после чего цровод т смыв культуры дл инокулировани ферментера.Corynebacterium simplex culture was grown in matrices on solid agar medium containing corn extract and glucose for 72 hours, after which the culture was washed to inoculate the fermenter.
Одновременно с этим выращивают культуру Bacillus megatherium и внос т кортизонацетат в количестве 3 г в 200 мг ацетона. Ферментацию провод т при перемещиванииAt the same time, a culture of Bacillus megatherium is grown and 3 g of cortisone acetate are introduced in 200 mg of acetone. Fermentation is carried out when moving
260 об/мин, подаче воздуха 1 л на 1 л среды в минуту и температуре 28° С. Продолжают ферментацию 20 час.260 rpm, air supply 1 l per 1 l of medium per minute and a temperature of 28 ° C. Fermentation is continued for 20 hours.
Контролем конца реакции служит хроматографическое разделение веществ на бумаге в системе форма.мид-бензол и окращиванне п тен раствором Люгол .The control of the end of the reaction is the chromatographic separation of substances on paper in the system with form.mid-benzene and the colored spot Lugol solution.
Ферментационный раствор экстрагируют 5 .1 хлористого метнлена. После удалени растворител осадок нерекристаллизовывают из изоиронилового спирта с углем. Получают 2 г нреднизона с т. пл. 228-229°. Вы.ход на двух стади х составл ет 75% от теоретического количества.The fermentation solution is extracted with 5 .1 methylene chloride. After removal of the solvent, the precipitate is non-recrystallized from isironyl alcohol with coal. Get 2 g nrednisona with m. Pl. 228-229 °. The yield in two stages is 75% of the theoretical amount.
5five
Предмет изобретени Subject invention
Снособ нолученн Д-дегндрокортизона (преднизона) из коргнзон-ацетата, отличающийс тем, что, с целью увеличени выхо0 да и упрощени технологического процесса, кортизон-ацетат одновременно дегидрируют и омыл ют микробиологическим иутем действием смещанной культуры Corynebacterium simplex u Bacillus megatherium ВНИХФП-1.The method for obtaining D-dendrocortisone (prednisone) from korgnzon-acetate, characterized in that, in order to increase output and simplify the process, cortisone-acetate is simultaneously used in the process of exchanging Corynebacterium simplex and Bacillus with syactectrohexacrythyne and in the bacterium -phacillone -phaculpha, which is used as a process by means of the Corynebacterium simplex and Bacillus.
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU129656A1 true SU129656A1 (en) |
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