SU1287871A1 - Arrangement for transplantation of embryo of the mammalia - Google Patents

Abstract

The invention can be applied for non-surgical embryo transfer in experimental embryology, gynecology and veterinary medicine. The purpose of the invention is to reduce genital trauma injuries and increase the safety of transplanted embryos. The device comprises a tube 1 and a rod 2 of elastic transparent material, a chamber 3 for embryos. The end of the rod has a spherical shape 4. Labels 6 and 7 are affixed to the tube and rod. For working with small animals, the device is equipped with a tube 5. The rod 2 is extended to open the chamber 3, into which the medium with the embryo is introduced. The device is inserted into the cervical opening with a working end. Progressing the rod 2 until the mark 7 is combined with the outer end of the tube 1, the chamber 3 is opened. After a few seconds, the device is turned around the axis and removed from the body. The spherical stem end and the smooth edges of the tube I pass through the muscle sphincter without any damage. 3 il. (6 (O N5 00 1 oo

Description

The invention relates to devices used for non-surgical embryo transfer, which can be widely used in experimental embryology and, moreover, in gynecology and veterinary medicine.

The purpose of the invention is to reduce trauma to the genital tract and increase the safety of transplanted embryos.

Figure 1 shows the device with its introduction into the uterus; figure 2 - the same, when laying embryos in the camera; on fig.Z - the same, when removing embryos from the chamber.

The device is a tube 1, the working end of which has rounded edges and in the cavity of which rod 2 is installed. Tube 1 and rod 2 are made of elastic transparent material. In the lateral part of the rod 2 there is a cavity forming the chamber 3 for embryos. The end of the rod associated with chamber 3 has a spherical shape 4 with a radius equal to the outer radius of tube 1. To facilitate work with small animals, such as mice, the tube is inserted into the outer tube 5.

The opposite ends of the worker (which, after insertion of the device into the animal's body will be external), the ends of the tube 1 and the rod 2 are marked with marking marks 6 and 7, respectively.

The proposed device operates as follows.

To transplant the embryos into the uterus of the recipient, the animal is immobilized and the working end of the tube 1 protruding from the tube 5 is inserted into the cervical opening. At the same time, the spherical end of 4 rods

 2 and the smooth edges of the tube 1 gently pass through the muscle sphincter, without causing damage. Further, advancing the tube 1 relative to the tube 5 and bringing the mark 6 to the outer end of the tube 5, the working

10, the end of the device (located in the position shown in Fig. 1) into the horn of the recipient. Then, moving the rod 2 until the mark 7 is aligned with the outer end of the tube 1 (Fig. 3), the chamber 3 is opened. At the same time, the embryos are able to fall into the uterine horn cavity. After waiting a few seconds, for better contact of the embryos with the uterus medium, the device is rotated around its axis in order to reliably ensure the release of embryos into the uterus.

2Q Then the working end of the device is carefully removed from the body. The transplantation is over. The animal immediately after transplantation can lead a normal life.

Claims (1)

25 Formula of Invention A device for the transplantation of mammalian embryos, containing a tube and a rod mounted with the possibility of reciprocating in 15 Moving the rod 2 and the tube 1 from the 30 tube, characterized in that reducing trauma to the genital tract and increasing the safety of the transplanted embryos; a cavity is formed in the lateral part of the rod, forming a chamber, with the end of the rod adjacent to the chamber. with respect to each other, open the chamber 3 (figure 2). Under visual control, medium with embryos was introduced into chamber 3. Then, moving the rod 2 relative to the tube 1, the chamber 3 is completely closed. In such In the position (Fig. 1), the device is ready for operation, has a spherical shape with a radius, and in this "packed form" can be delivered to the place of transplantation. The outer radius of the tube, and the tube and rod are made of elastic material. To transplant the embryos into the uterus of the recipient, the animal is immobilized and the working end of the tube 1 protruding from the tube 5 is inserted into the cervical opening. At the same time, the spherical end of 4 rods 2 and the smooth edges of the tube 1 gently pass through the muscle sphincter, without causing damage. Further, advancing the tube 1 relative to the tube 5 and bringing the mark 6 to the outer end of the tube 5, the working the end of the device (located in the position shown in Fig. 1) into the horn of the recipient. Then, moving the rod 2 until the mark 7 is aligned with the outer end of the tube 1 (Fig. 3), the chamber 3 is opened. At the same time, the embryos are able to fall into the uterine horn cavity. After waiting a few seconds, for better contact of the embryos with the uterus medium, the device is rotated around its axis in order to reliably ensure the release of embryos into the uterus. Then, the working end of the device is carefully removed from the body. The transplantation is over. The animal immediately after transplantation can lead a normal life. Invention Formula A device for the transplantation of mammalian embryos, containing a tube and a rod mounted with the possibility of reciprocating in tube, characterized in that The outer radius of the tube, and the tube and rod are made of elastic material.  2Vus. 3 VNIIPIZakaz 7734/6 Circulation 617 Subscription Production and printing company, Uzhgorod, st. Project, 4