HU202398B - Method and inseminating needle for natural and/or cerviconterinal inseminating sheep rutting naturally or synchronized as well as super-ovulated ones - Google Patents

Abstract

The invention relates to an apparatus for cervicouterine insemination of ovines (sheep). This apparatus comprises a syringe, a rod which is connected thereto and a hollow fertilisation needle which comprises at the tip a spherical head. The said apparatus is inserted 60 to 70 mm through the neck of the uterus of fertile sheep, kept in the presence of "guinea-pig" rams (sexually active but made incapable of fertilisation selected on the basis of the tolerance reflex, by turning it clockwise. <IMAGE>

Description

The present invention relates to a method and an inseminator needle for natural and / or synchronized reproductive (induced) and superovulated sheep for natural and / or cervicouterinal insemination. The inseminating needle is attached via a needle tip to the stem and the stem is connected to an injection syringe, thereby providing insemination, depending on the number of fertile sperm cells in the inseminating dose, the length of the germ, synchronization and superovulation of the germ.

Because of the anatomical features of ewes (eg, the inseminator cannot fix the cervix through the rectum) and because of the complex structure of the cervix, the well-established deep cervical-uterine fertilization method cannot be used in cows. The goal is the same: it can be easily applied anywhere, with artificial insemination, using a small number of fertile sperm.

Naturally reproductive or reproductive. cervical fertilization is a successful, practical procedure for synchronized herds, but it requires a large number of sperm (80 million or more) Fukui and Roberts, and Andersen et al. the method of transcervical intrauterine insemination is too tiring and difficult and is therefore not considered as a possible alternative to the conventional cervical technique (Andersen, K. Amadal, J., Fougnes, J. A. (1973) Zuchtygiene, 8, 113-118; Fukui, Y., Roberts, Ε. M. (1976) Proc. Int. Congr. Sheep Breed, Muresk, 482-494.]

Cervical insemination of superovulated embryo donor ewes is not used due to unsatisfactory results. An effective method, insemination by laparotomy, has the significant disadvantage of producing congenital growths. Therefore, donor mothers can only be used in 2-3 programs. This disadvantage is overcome by laparoscopic insemination [Cseh S. et al. al .: Journal of Hungarian Veterinarians, 1941, 1968. (1) 55-57]. Laparoscopic insemination is not a simple, expensive procedure.

At the 1984 conference of the European Animal Breeders' Association in Debrecen, Tasi and Resli presented the cervicouterinal insemination method, the insemination tool developed and the results of experiments conducted with it. Changes in input depth in the cervix:

Device Name Number of Nuts Average Input Scatter depth (cm) + Milovanov's glass pipette CASSOU's 254 1.95 0.98 gun Crafted ins 584 2.91 1.10 mining tool 100 4.25 1.24

(Zs. Tasi - I. Resli: International Conference on Large-scale Sheep Breeding, 14-16 April 1984, Debrecen Publication, 183-194).

The method was recommended by the conference for practice (Gy. Szovátay: Aeü. Tak. Közi. 1986/1. XII. 47-49). Due to the size of the device it is not suitable for ewes. All herds with lower rates of uterine entry did not produce satisfactory results.

No data have been found in the literature on the use of long term ejaculation.

It is an object of the present invention to provide a method and device that results in a simple and low-cost, high-efficiency fertilization: naturally reproducing, synchronized (induced), and superovulated, embryo-donor mothers, sometimes in the lamb.

The present invention is based on the discovery that the efficiency of cervicouterinal insemination can be significantly increased by biotechnology. That is, the lasting ewes during the ejaculation increase the depth of intake and improve the productivity by stimulating the physiological processes. Long-lasting rum exposure is achieved by placing sexually active rams (vasectomized) between mothers sexually active but incapacitated during fertilization. Only the sex selected on the basis of the tolerance reflex is eligible for our method.

The present invention provides a method for the natural and / or cervicouterinal insemination of naturally or synchronously reproduced (germ-induced) and superovulated sheep; rams are sexually active but are incapacitated during insemination, and in the meantime are mated with breeders and / or cervicouterinally inseminated.

The invention further provides an insemination device adapted for carrying out the method of the invention: a needle which is connected to a transparent hollow stem known per se by means of a needle head and which is connected to a syringe containing semen.

The insertion needle is 70-100mm, preferably 80-90mm long, 1.0 + 0.1mm in diameter, 0.14mm in wall thickness, preferably stainless steel, one end of the straight end of the straight end of the sperm holding straw and the other end of the end of the spiral end spherical end, two curvature of the spiral end of the straight pipe end

3.2-3.6 mm, preferably 3.5 mm radius, the first curvature of the spiral end closes to 4050 ° with the second curvature bent at 60-65 °, and the spiral end is counterclockwise 90 It bends at -100 ° and closes with a spherical end.

Connected to the needle head of a gas insertion needle is a 200250 mm long hollow stem, which is a transparent polypropylene tube, 5.5-3.2 mm in diameter.

In addition, a 2-10 ml plastic injection syringe is conveniently attached to the hollow stem.

The device according to the invention is illustrated in Example 1, with the help of Figs

Fig. 1b is a sectional view of the inseminating needle, Fig. 2 shows the inseminating means of a sheep. The process of the invention is illustrated by way of Examples 2 and 3, but is not limited thereto.

Example I

The sheep inseminator is shown in FIG. 2, having a hollow stem B attached to it with an insertion needle A at one end and an injection syringe C at the other end.

The insertion needle A is a 90 mm long, 0.1 ± 0.1 mm thick stainless steel tube with a diameter of 0.1 ± 0.1 mm. One end of the straight tube end 1 of the insertion needle A is closed by the needle head 2 for securing the hollow stem B, which holds the semen holding straw, and the other end is closed by the spherical end 3 of the spiral tube end 4

EN 202398 Β is soldered. The spiral tube end 4 forming a continuation of the straight tube 1 has two curves with a radius of 3.5 mm. The first curvature of the spiral end 4 is 45 ° relative to the straight end I, the second curvature is inclined 65 °, the spiral end 4 is rotated 90 ° counterclockwise and 3 is a spherical end having a diameter of 2, 2 mm.

The hollow shaft B is a transparent, hard polypropylene tube 200 mm long with a diameter of 4 mm.

The C injection syringe is a plastic 10 ml (Record conosu) syringe made of plastic.

The inseminating needle A of the present invention is used to open the vagina with a vaginal mirror and to locate its outer uterus. Subsequently, a small amount of air is drawn into the injection syringe C and the hollow shaft V is inserted, and the semen is inserted into the hollow shaft B and the insertion needle A is inserted.

Locate the cervix on the outer uterine cervix and rotate it clockwise a quarter of a turn until it collides. The semen is then injected.

Example 2

We experimented with super-ovulated embryo-donor ewes of the lacaune breed between 1 January to 31 March and 1 September to 1 December.

At regular intervals, mating mothers on the 10th day after mating are at 1,250-1,500 U.I. PMSG injection, on day 12, 2.5 ml Enzaprost-F injection (CHINOIN) was administered.

We placed trial mothers among the programmed mothers. At 9 o'clock on day 15, mothers with tolerance reflex were first inseminated at 15 o'clock, and at 9 o'clock the next day, mothers with unchanged tolerance reflex were re-fertilized by cervicouterinal insemination.

The females were divided into two groups. In one group, a test cock is placed during the time of gestation and not in the other.

Fresh, undiluted sperm from 0.2 to 0.3 ml was used for cervicouterinal insemination.

Cervicouterinal insemination was performed by exposing the vagina with the vaginal mirror and contacting the external uterus.

The insertion needle was inserted into the cervixuteri and rotated in a clockwise direction with firm pressure and inward until it encountered explicit resistance, or approximately. A depth of 7 cm was reached. The semen was then injected.

The uterine washes were surgically removed on day 21. The way in which the ova were evaluated was the same for each group. The number of mothers per group was 40. The results were biometrically processed.

According to our studies, during cervicouterinal insemination the fertility of the ova is 80.7% in the season and 27.0% in the case of non-cough. In the same alternate season, 67.5% and 25.9% respectively. The difference is 53.7% for the season and 41.6% for the offseason. The differences are significant at P-0.1%.

Example 3

During the periods described in Example 2, the same breed and the same superovulated embryo-donor ewe were experimented on.

We also placed trial mothers among the mothers in the program. At 9 o'clock on the 15th day, the mothers showing tolerance reflexes were first reassessed, at 15 o'clock the second time, and at 9 o'clock the next day, the mothers showing unchanged tolerance reflexes were re-covered for the third time.

The females were divided into two groups. In one group, a test-boar was placed during the time of gestation and not in the other.

The uterus was washed by surgery on day 21. The way in which the ovum was evaluated was the same for each group. The number of mothers per group is 40.

The results were processed by biometric calculations.

During the season ovum fertilization was 67.9% and 10.7% without cough. The difference is 57.2% in favor of coughing. In the offseason, 67.9% fertility is increased by rearing. The differences are significant at P = 0.1%.

The present invention has several advantages over known methods and tools:

1. The method and insemination needle are suitable for cervicouterinal insemination of sheep in both fertile lambs and mothers.

2. Using the method and inseminating needle, a fertilization dose of substantially lower cell number can be achieved or better.

3. A superovulated embryo donor mother can be efficiently inseminated by eliminating damage.

4. The device is serially manufactured and inexpensive.

5. The procedure and handling of the insertion needle is simple, quick and requires no special expertise.

Claims (2)

PATENT CLAIMS CLAIMS 1. A method of naturally and / or cervicouterinally inducing naturally, synchronously breeding, and superovulated sheep, characterized by inserting sexually active but ineligible rams during the breeding season and breeding or breeding at the desired time. cervicouterinal insemin. Inseminating needle which is connected via a needle head to a hollow shaft, the shaft being connected to an injection syringe, characterized in that the insertion needle (A) is 70-100 mm long, preferably 80-90 mm long, with a diameter of 1.0 ± 0.1 mm. 14 mm wall tube, preferably stainless steel tube, one end of the straight tube end (1) of the inseminating needle (A) securing the hollow stem (B) of the semen holding straw (B) and the other end 3 of the end (4v) of the spiral tube end ), the first curvature of the spiral tube end (4) has a radius of 3.2-3.6 mm, preferably 3.5 mm, the first curvature of the spiral tube end (4) being 40-50 °, with a second curvature bent at 6O-65', the spiral tube end (4) anticlockwise 90 ° to 100 ° and closed by a spherical end (3).