SU1257444A1 - Method of producing three-dimensional stereoscopic presentation of biological objects and its internal structures - Google Patents

Abstract

The invention relates to methods for producing a three-dimensional volume image of biological objects. The purpose of the invention is the spatial localization of histochemical components. Get two rows of slices. On one of the rows of glasses with slices, a histochemical reaction is performed. Sections of another row are stained in the usual manner. Sketch the contours of the histological structures studied. These sketches are combined when sketching traces of a histochemical reaction. Sheets of paper with combined sketches stick on wax plates. Prepared slice models. On the basis of these models make a reconstruction model. (L

Description

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The invention relates to biology and medicine and is intended for use in scientific and diagnostic research.

The purpose of the invention is the spatial localization of histochemical components.

The method is carried out as follows.

Two rows of slide glasses are prepared in advance and in each row they are numbered continuously. In the manufacture of a series of cuts, the latter are positioned alternately (through one cut), then on one row of glasses, then on the other. Thus, instead of one row of consecutive cuts on several slides (as practiced in the manufacture of a series of histological specimens), two rows of cuts are obtained, arranged through one slice in each row of glasses. On one of the rows of glasses with slices, a histochemical reaction is set up, and sections of another row are processed using conventional histological staining methods. The numbered sheets of paper (at the required magnification) are sketched the contours of the histological structures under study and guide pins, then by combining the same numbering sheets of paper with the sketched outlines of the histological structures along the course guides, they traced the histochemical reaction marks, using colored pencils or felt-tip pens. Sheets of paper with combined sketches are glued onto wax plates (since slate sketches were made on two sheets of paper from two sections, the thickness of the wax plates should be doubled, respectively, than in conventional model making) those. cut out the contours of the investigated structures. For a clear orientation, the location of the colored spots of the histochemical reaction is indicated on the edge of the wax plates (cut models) by specially prepared (painted and melted) wax. Based on the obtained slice models with guide marks, a structural model is made.

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Example, Pre-prepare two rows of slides: row A and row B. In each row, continuous numbering of glass 5 is made. A series of histological sections are prepared from the object under study, for example, buds of the embryo, with guiding guides placed in the block (two kapron colored threads), alternately after one row of row A, then row of B (for example, even cuts in the order of their cutting are placed on row A, odd - on row B). Thus, two rows of glasses are obtained with sections cut through one in each row. Sections of row A are processed in the usual histological method of staining, for example, according to Van Gieson, and in sections

20 rows yes B produces a histochemical reaction, for example, to succinate dehydrogenase. On the numbered sheets of paper with the necessary magnification from sections A and A, the contours of the object under study, for example, the nephron canaliculi, are sketched on the sheet of paper K 1, the first section is drawn .; on sheet number 2 - the second cut, etc. Then, with the same increase, comparing row B of the guidelines, the traces 35 of the histochemical reaction are sketched on the corresponding numbering sheets of paper with sketches of the histological structures, using colored pencils or phlomas- (. Terry: for example, blue

places of accumulation of precipitates of blue color (diphormazan), red - monoformazan. Sheets of paper with combined .glues are glued onto wax plates and cut out slice patterns from them. The thickness of the wax plates should be twice as large (according to an increase in sketches), since the data obtained from two sections are combined in one sketch. At the ends of the models of sections prepared, preliminary prepared (marginal and molten) wax denote the location of the spot — an accumulation of sediments of the studied biologically active substance (diformazan and monoformazan). A wax reconstructive model of the forming tubular part of the nephron with

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localization of clusters of succinic, scientific, and diagnostic isnatdehydrogenase, as well as their form of research,

Claims (1)

in volumetric image and dimensions. The formula of the invention The proposed method makes it possible to obtain clear information on the method for obtaining a three-dimensional volumetric distribution in a dimensional image of biological individual organs and tissues of the organism and its internal structures enzymes, nucleic acids, mic by staining histological prepar elements and other biologicals, sketching contours and its inactive compounds. Urien structures on paper with the use of the method allows us to follow the guidance of the house of qualitative changes containing them, reconstructing them into wax plates and localizing biologically aaaaa and making reconstructive tive compounds in the normal-model process, differing of the body’s vital functions in the 15th that, for the purpose of spatial Different extreme conditions. Histochemical composition localization. The invention can be used, one of the adjacent sections is based on the experimental-histochemical processing.