SU1255585A1 - Method of biochemical purification of waste water from organic substances - Google Patents

Description

The invention relates to the treatment of wastewater, and more specifically to the biochemical treatment of wastewater from organic matter, and can be used to purify wastewater from the manufacture of synthetic products and vitamins, biochemical preparations, chemicals, etc.

Due to the increase in production capacities and the expansion of the range of injected products, the quality and quantity of wastewater composition becomes more complicated, and the concentration of contaminants dissolved in them increases.

The purpose of the invention is to simplify the process due to the appearance of mutant mTHNMOBs with increased activity at a similar degree of purification.

The method is carried out as follows.

The x1-gm mutagen - dimethyl sulfate in the ratio mutagen: active silt i: 15000 - 1: 20000 is introduced into the active volume of 0.001-1% of its total volume in the aeration tank. The mixture of activated sludge with dimethylsulfate azrirot is for 2-5 hours at 15 - 24 s. Then another chemical mutagen is added - hydrogen peroxide in a ratio of mutagen: activated sludge 3: 1-5000 - 3: 20000 and the mixture is aerated another 10 - 12 h at the same temperature. Thus processed sus1: 4: 15000

Of the thousands of proven colonies.

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Penza sludge is mixed with with the total volume of sludge. The sludge treatment interval is 10-15 days. The active sludge for the treatment of mutaghe5 is taken in the amount of 0.001–1%, which ensures work with both small and large volumes of aerotanks.

Treatment or two mutagens.

10 and successively initially with dimethyl sulfate and then with hydrogen peroxide, provides a higher incidence of mutant strains with increased activity. With

By successive effects on active sludge by dimethyl sulfate and hydrogen peroxide, a significant increase in the mutagenic effect of hydrogen peroxide occurs.

Mutagens are taken in an amount determined by the ratio of dimethyl sulfate: hydrogen peroxide: activated sludge 1: 3: 15,000 - 1: 3: 20,000, which provides effective doses of mutagens, causing high hereditary variability on the basis of an increase in the enzymatic activity of the sludge at maximum survival.

Data of microbiological studies on the occurrence of active strains in the treatment of sludge with mutagens in various ratios during exposure 2 - I oh are presented in table Table 1

170

17.0

3

The treatment with dimethyl sulfate is carried out within 2–5 hours, and with water peroxide of the genus 10–12 hours, which ensures the occurrence of the maximum mutation spectrum.

A temperature in the range of 5 to 24 is optimal for the viability of microorganisms.

The frequency of processing of activated sludge every 10-15 days ensures the stability of mutant cenoses under changing conditions in the running aeration tank.

Example 1. From a working aerotank with a capacity of 10 liters, 100 ml of a suspension of activated sludge with a concentration of 3 g / l are taken, 0.005 g of dimethyl sulfate is introduced and aerated for 2 hours at room temperature (20 ° C). Then, 0.5 ml of a 3% hydrogen peroxide solution is added to this mixture and another 1 O hour is aerated. The activated sludge thus treated is returned to the aerofoil, where waste water from the production of synthetic intermediates and vitamins containing organic matter is fed. COD 2022. After 7 days, the COD purification degree reaches 97.1% versus 74.0% under conditions of untreated sludge. .

From table 2 it follows that the proposed method of wastewater purification Pu555854

EXAMPLE 2. From an aerotank with a volume of 1 O l, 50 ml of a suspension of active sludge are taken, 0.004 g of dimethyl sulfate is introduced, aerated for 4 hours, 5 0.4 ml of 3% hydrogen peroxide solution are added, and they are air-cooled for 12 hours. and the mixture is introduced into the aeration tank, to which waste water containing organic substances is supplied to the HS (2067 Mr0.j / l. The degree of purification by COD reaches 98.3%,

Example From an aerotank with a volume of 7000 m, 100 l of sludge liquid is pumped out into a separate container, processed as in example 2 and returned to the aeration tank, where the wastewater from COD 1078 mg02 / l is fed. After 7 days, the degree of purification by COD is 90.8% of Proto1 76.1% under conditions of untreated sludge.

20

Example 4. Treatment of activated sludge with mutagens is carried out analogously to example 3. Wastewater has a COD 1302 MG07 / L. The degree of purification reaches 88.9% versus 72.1% in conditions of raw sludge.

Comparative results of the effect on active sludge separately of dimethyl sulfate, hydrogen peroxide, as well as their combined effects are presented in Table 2.

table 2

the combined effect of two mutagens on the activated sludge - di51

methyl sulfate and hydrogen peroxide can increase the effect of wastewater treatment of synthetic intermediates and vitamins up to 98.3% versus 90.5% and 95.0% when activated sludge is treated only with methyl sulfate or hydrogen peroxide, respectively.

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In tab. 3 shows the results of studies on the treatment of wastewater from organic substances with activated sludge, partially treated with two 5 mutagens, depending on the concentration of mutagens and the processing time. The studies were carried out in laboratory aeration tanks with a volume of 1 l; the amount of treated sludge was 1%.

Table 3

dms

but

dms

on.

dms

dms dms

but,

dms

but

1: 12000 3: 12000 1: 15000 3: 15000 1: 20000 3: 20000

1: 12,000 3: 12,000

1: 15000 3: 15000 1: 20000 3: 20000

The use of mutagens in the case of illicit sludge by two mutagens in low concentrations leads to a decrease in the different sequence of the degree of wastewater treatment. at various times

Table 4 presents comparisons and ratios of mutagens: data on effects on active sludge.

Table A

210

130

161

237

82

66

82.4 89.1 86.5

82.1 93.8

95.0

ten

12

12

12

12

ten

eight

ten

ten

85.3 82.7 97.4 96.8 81.3 85.2 76.3 84.2 98.1

Table 5 presents data on

The quality of the active roten that is being processed,

In tab. Figure 6 presents the data of hydrogen acid when changing the sequence to enhance the mutagenic effect of the sequence of introduction of mutagens.

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0 Continuation of table 4

25

Sludge for various volumes of a table 5

eleven

Of the thousands of proven colonies. Editor I. Derbak

Compiled by G. Lebedev Tehred M. Khodanych

4780/26

Circulation 864 Subscription

VNIIPI USSR State Committee

for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5

Production and printing company, Uzhgorod, st. Project, 4

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12 Table, 6

Corrector V.Sinitska

Claims (3)

1. WAY OF BIOCHEMICAL WASTE WATER TREATMENT FROM ORGANIC COMPOUNDS, including treating part of the activated sludge with a chemical mutagen — hydrogen peroxide, introducing it into the total volume of activated sludge and mixing, characterized in that, in order to simplify the process, mutant strains with increased activity appear with a similar degree of purification, part of the activated sludge is treated sequentially with dimethyl sulfate and hydrogen peroxide in the ratio dimethyl sulfate: hydrogen peroxide: activated sludge 1: 3: 15000 1: 3: 20,000. 2. The method of pop. 1, characterized in that the treatment with dimethyl sulfate is carried out for 2 to 5 hours, and hydrogen peroxide for 10 to 12 hours. 3. The method according to PP. 1 and 2, about t l - characterized in that the activated sludge is treated in an amount of 0.001-1 vol.%. <»SU on 1255585