SU1242520A1 - Method of preparing nutrient medium for growing fungi - producers of ferments - Google Patents

Description

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The invention relates to the microbiological industry, in particular to the production of enzymes, and can be used in the preparation of amylolytic enzymes for the alcoholic production of cellulolytic and pectolytic enzymes for agriculture.

The purpose of the invention is to prevent the development of coccal infection during the cultivation of enzyme producers, as well as to obtain a complex of broad spectrum enzymes.

In the production of biomitsin, a significant amount of waste is formed in the form of a culture fluid with a residual biomitsin activity of 50–90 units / ml, which is discharged to treatment plants, which inhibits the microflora of treatment plants and they do not cope with the treatment of industrial wastewater. The utilization of waste from biomitsin production will improve the conditions of wastewater treatment by eliminating the process of inhibition of microflora treatment plants.

The essence of the invention is that the nutrient medium for cultivation of fungi-producing enzymes, containing a carbon source, a nitrogen source and mineral components, additionally introduces biomycin production waste in the form of a culture fluid with a residual biomitsin activity of 40-50 units / ml in the amount 3-25% by volume

Example 1. For the cultivation of producers of amylolytic enzymes, a hydrolyzate of xxano-wheat flour and wheat bran is prepared, a corn extract of 0.5% is added. Waste of biomitsin production is introduced in the amount of 10% of the volume of the medium, the volume of the medium is adjusted to 1 l with water. The medium prepared in this way is sterilized, cooled and seeded with Aspergillus awamori F-122 - producing amylolytic enzymes. Cultivate at 28 ° C with aeration 1 volume of air per 1 volume of medium for 72 hours. A deep culture with o activity is obtained. -amylases 15.8 units / ml and glucoamylases 31.3 units / ml.

When cultivating the same strain on the medium of the indicated composition, but without adding waste bio-production, the activity of the deep

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cultures: f-amylase 18, units / ml, glucoamylase 26.3 units / ml.

EXAMPLE 2 Prepares the medium as described in Example 1, but 25% of the biomycin production waste is additionally introduced. The medium is sown with the same strain and cultivated under the conditions described.

An in-depth culture is obtained with the following activities: s / -amylases and glucoamylases, respectively, 12.9 and 25.4 units / ml.

P. p and me R 3. Mixed in water are 4% beet pulp, 1.8% malt to sprouts, 5% grain-potato stillage, (U1., ,,) ,, 50 „0.6%; KN, RO, 0.2%; M, SO, | About 06. .. and 3% culture liquid -: Bones5 - a waste of biomitsichito production. The resulting medium is sterilized, cooled and seeded. Trichoderma viride 13/10, Cultivated with aeration for 168 h.

Cellulase activity on the hydrolysis of filter paper 18.3 e d / ml, E (5).: When cultivating the same strain under the described conditions on a medium prepared without waste of biomycin production, the filterula activity on the hydrolysis of filter paper is 18.1 units / ml

Example .4. Mix 3% beet pulp, 10% extract of malt sprouts, 0.7% ammonium sulphate, 0.1% potassium phosphate, 0.05% magnesium sulphate and 7% waste biomitsinovogo production in the form of culture fluid. The medium thus obtained is sterilized, cooled before, and seeded with a producer of psitolytic enzymes LzreggilLus foetidus.

The strain is cultured by aeration for 120 hours. The pectolytic activity in the filtrate of the culture fluid is 4.2 U / ml (the interferometric method).

In the control experiment (without adding waste biomycin production) Pectolytic activity of 4.13 units / ml.

PRI me R 5. The sowing was carried out on the working environment used in the production of the amyticolytic complex of enzymes (in example 1) containing 2.5% of agar, the isolation of the Streptococcus coccal infection and its accumulation in the same production medium. within 72-80 hours

3 The obtained culture liquid with a titer of 1x10 mlr / ml in the amount of% is inoculated simultaneously with a suspension of Aspergillus awamori F-122 spores in 700 ml flasks containing 100 ml of the production nutrient medium used to obtain

amylolytic enzyme complex. Four flasks were injected with streptomycin in the amount of 1250 units / 100 ml, and four bottles were introduced into the flasks: - 250 ml of each of the biomecin production (chlorotetracycline) with a residual biomitsin activity of 50 units / ml (i.e. 100 ml of 1250 units), cultivated for 72 hours, samples are taken and the presence of coccal infection is microscopically determined.

The effect of antibiotics on activity

ekormentov and titer coccal infection

Presented in the table.

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Streptomycin

Waste from the production of biomitsin in the form of a filtrate (chlortetra-cyclin)

Editor V. Petrash

Compiled by E. Vorobyova

Tehred O. Gortvay Proofreader O. Lugova

Order 4092 Circulation 490 Subscription

VNIISH State Committee of the USSR

for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5

. Production and printing services Uzhgorod, st. Project, 4

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 The use of waste proizpodn.ch biomitsin in the form of a filtrate with a residual activity of 50 units / mp in an amount of 25% by volume of nutrient media reduces the amount of unwanted coccal infection, while maintaining the glucoamylase activity and the required ratio of them in the enzyme complex.

Thus, the proposed method eliminates non-sterile processes (coccal infection) in the production of such enzymes as Amylo-glucavamorin Gx, Pectofoetidin GZx, Cellovyridine GZh, and also improves the operation of the wastewater treatment plants, since the discharge into the sewage system of waste, biomycin production inhibits useful microflora treatment facilities, which leads to a decrease in the quality of wastewater treatment.

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Claims (4)

1. A METHOD FOR PREPARING A NUTRIENT ENVIRONMENT FOR CULTIVATION OF MUSHROOM PRODUCERS OF ENZYMES, which involves mixing sources of carbon, nitrogen and mineral salts in water, characterized in that, in order to prevent the development of coccal infection during the cultivation of the producer, as well as to obtain a complex of broad-spectrum enzymes, in the environment is additionally introduced waste biomycin production in the form of a culture fluid with a residual activity of biomycin 40 50 units / ml in an amount of 3-25 vol.%. 2. The method of pop. 1, characterized in that for the cultivation of producers of amylolytic enzymes waste biomycin production is introduced in quantity. 1.0 - 25%. 3. The method of pop. 1, characterized in that for the cultivation of producers of cellulolytic enzymes waste contribute in the amount 3 - 8%. 4. The way to sing. 1, characterized in that for the cultivation of producers of pectolytic enzymes waste contribute in the amount of 6 to 10%. >