SU1212972A1 - Method of preventing growth of bacteria - Google Patents

Description

one

The invention relates to the treatment of fluids for the suppression of the viability of various microorganisms that cause bio-damage to materials and technical structures, and can be used in various branches of the national economy, including in the oil industry during drilling and oil production.

The purpose of the invention is to increase the degree of prevention of bacterial growth.

9-Alkylsuccinic carbazole belongs to the class of anion active surfactants. The compound is a mixture of acids, mainly tri- and tetrasulfonic acids, soluble in water, stable in solutions of mineral acids and alkaline, as well as in the presence of sodium chloride and hardness salts.

Example. In 2-3 days. cumulative culture of sulfate-reduced bacteria (CBB), isolated from oilfield media, 9-alkylsulfonic acid of carbazole is added. After daily surcharge at 32 ° C, 1 M.II liquids are taken from these samples, injected into vials with Postgate nutrient medium, and thermostatted during. 15 days As a control, use samples without the addition of reagent. The bactericidal activity is assessed by the absence or presence of darkening of the samples, and the absence of darkening indicates a complete inhibition of the growth of sulfate reducing bacteria.

The experiment was performed in triplicate.

With all quantities of the reagent injected (0.01-2.0 wt.%) The bactericidal activity of the reagent is high, SSCs are completely absent, while if you enter the bactericide by a known method, even in the amount exceeding the indicated growth, their growth is suppressed. %

2129722

EXAMPLE 2 9-alkylcarboxylic acid 5-ash is added to flasks containing the museum culture of the bacteria Pseudomonas fluorescens VKMB-561. After daily incubation in a thermostat at 25 C, 0.1-0.5 mp of liquid is taken from the samples studied, seeded on Petri dishes with solid nutrient medium, prepared 10

Noah from dry nutrient agar

(SPA) and incubated for 5 days. in the thermostat. The effectiveness of the reagent for suppressing this culture is assessed visually by the absence or

15 the presence of colonies on the surface. Nutrient agar.

Using a similar method, the biological effect of the recommended reagent on the museum cultures of the bacteria Bacillus subtilis BKMB-428 is investigated; Mycobacteryl flavum var. me- thanicum BKMB-856.

The test results are presented in the table.

25 Example. Waste oil field water pumped into the reservoir to maintain reservoir pressure and containing a total amount of microorganisms of 10 cells / ml, including:

30 SCU units; heterotrophic microorganisms - des; hydrocarbon-acidifying microorganisms (ten and others), are treated with carbazole 9-alkyl sulfonic acids at a concentration of

jr Oh, 1 wt.% and incubated at 25 C in

for 1 day. The effectiveness of inhibition of microorganisms is assessed by the effect of the reagent on the indicated control groups of bacteria.

Q Suppressing the growth of SIS is determined according to the procedure described in Example 1. The presence of heterotrophic and hydrocarbon-oxidizing bacteria is determined by limiting dilution method, using peptone water and Norenkova medium, respectively. After processing, viable bacterial cells are absent.

Noah from dry nutrient agar

Culture

Pseuboinonas fluorescens VKMV-561

Mycobacterimn flavum var. methanicinn BKMB-856

Bacillus subtilis. BKMB-428

Note. - Lack of colonies of bacteria in the nutrient

agar.

+ Presence of bacterial colonies on nutrient agar.

Concentration, May. %

0.01 0.1 To, 5 GiO T2.0

Claims (2)

1. METHOD FOR PREVENTING BACTERIA GROWTH by treating with a bactericide, characterized in that, in order to increase the degree of prevention, carbazole 9-alkyl sulfonic acids of the general formula where R is alkyl C; x is H, S0 _} H; Y - SO ₃ H. 2. The method of pop. 1, distinguished by the fact that the bactericide is used in an amount of 0.012.0 May. %