SU1156691A1 - Method of obtaining lipoids from blood cells - Google Patents
Method of obtaining lipoids from blood cells Download PDFInfo
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- SU1156691A1 SU1156691A1 SU792869110A SU2869110A SU1156691A1 SU 1156691 A1 SU1156691 A1 SU 1156691A1 SU 792869110 A SU792869110 A SU 792869110A SU 2869110 A SU2869110 A SU 2869110A SU 1156691 A1 SU1156691 A1 SU 1156691A1
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- SU
- USSR - Soviet Union
- Prior art keywords
- blood cells
- lipoids
- obtaining
- methanol
- water
- Prior art date
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
СПОСОБ ПОЛУЧЕНИЯ ЛИПИДОВ ИЗ КЛЕТОК КРОВИ путем экстракции однофазной системой растворителей хлороформ: метанол: вода, отличающийс тем, что, с целью повышени выхода и сокращени времени получени целевого продукта, перед экстракцией в суспензию клеток внос т сернокислый натрий в концентрации 0,3- 1,0 М.A method for producing lipids from blood cells by extracting chloroform: methanol: water with a single-phase solvent system, characterized in that, in order to increase the yield and shorten the time required to obtain the target product, sodium sulfate is added to the cell suspension at a concentration of 0.3-1, 0 M
Description
СПSP
оabout
Изобретение относитс к биохимии, а именно к способам экстракции липидов.This invention relates to biochemistry, in particular to lipid extraction methods.
Цель изобретени - повышение выхода и сокращение времени получени целевого продукта.The purpose of the invention is to increase the yield and reduce the time to obtain the target product.
Пример. Донорскую кровь, стабилизированную цитратным раствором ЦНИИГПК-76 центрифугировали при 1500 об/мин на холоде 20 мин. Осадок клеток дважды промывали физиологическим раствором, суспензиру в холодном 0,9% NaCl (1:3 по объему), и центрифугировали при 1500 об/мин в течение 15 мин.Example. Donor blood stabilized with citrate solution of TsNIIGPK-76 was centrifuged at 1500 rpm in the cold for 20 minutes. The cell pellet was washed twice with physiological saline solution, suspended in cold 0.9% NaCl (1: 3 by volume), and centrifuged at 1500 rpm for 15 minutes.
Затем смешивали 2 мл клеточного осадка, 300 мг Na2SO4, 2 мл НаО и 15 мл смеси хлороформ: метанол (2:1 по объему). Смесь центрифугировали, экстракт отбирали, а осадок в течение часа экстрагировали повторно смесью хлороформ: метанол: вода (1:2:0,8 по объему). Экстракт разбавл ли в делительной воронке водой (4:1 по объему) и после встр хивани и последующего отстаивани отбирали нижний слой, который смешивали с бензолом (1:1 по объему) и упаривали при 40° по получени липидов в количестве 96% от их общего содержани в клетках.Then, 2 ml of cell sediment, 300 mg of Na2SO4, 2 ml of NaO and 15 ml of chloroform: methanol (2: 1 by volume) were mixed. The mixture was centrifuged, the extract was taken, and the precipitate was re-extracted with chloroform: methanol: water (1: 2: 0.8 by volume) for an hour. The extract was diluted in a separatory funnel with water (4: 1 by volume) and after shaking and subsequent settling, the lower layer was taken, which was mixed with benzene (1: 1 by volume) and evaporated at 40 ° to obtain lipids in the amount of 96% of their total cell content.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU792869110A SU1156691A1 (en) | 1979-11-23 | 1979-11-23 | Method of obtaining lipoids from blood cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU792869110A SU1156691A1 (en) | 1979-11-23 | 1979-11-23 | Method of obtaining lipoids from blood cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1156691A1 true SU1156691A1 (en) | 1985-05-23 |
Family
ID=20872012
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU792869110A SU1156691A1 (en) | 1979-11-23 | 1979-11-23 | Method of obtaining lipoids from blood cells |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1156691A1 (en) |
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1979
- 1979-11-23 SU SU792869110A patent/SU1156691A1/en active
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