SU1155618A1 - Clostridium felsinium 84 bacteria strain used in flax wetting - Google Patents

Abstract

The strain of bacteria Clostridium felsineum 84 (All-Union Collection of Microorganisms of the Institute of Biochemistry and Physiology of Microorganisms of the Academy of Sciences of the USSR, collection number VKM B 1527D) used for flax lotion. g (/) s

Description

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ABOUT)

111556

The invention relates to the microbiological industry, namely, to the production of active spore-based materials for flax lobe and is a new bacterial strain used in flax lotion.

The culture of Clostridium gurfelli is well known, note with flax loaf f,.

However, it is not capable of decomposing pectic substances in an acidic medium, when thermally wetted, since they do not synthesize the enzyme polygalacturon.

The closest to the proposed IS is the Clostridium felsineum 13 strain (ARRIAD), used for the locking and capable of destroying the pectic substances E2I1.

The disadvantages of this strain are the low activity of the pectolytic enzymes produced by it, inability to flax lobe in the regenerated cleaning fluid, as well as instability during storage, leading to the formation of various morphological forms and small spore formation during industrial cultivation.

The purpose of the invention is to increase the activity of the spore preparation for flax seedlings.

The goal is achieved by the Cloetridiwn felsineum 84 bacteria strain (All-Union Microorganism Collection of the Academy of Sciences of the USSR, collection number VKM B 1527D) used in

flax loaf.

The strain of bacteria Clostridiiaa felsinevm 84 is isolated with flax straw.

The resulting strain was identified 40 as defined by Verger (1974) and Prevot (1966) and has the following morphological, cultural, and physiological characteristics.

Morphological and cultural signs. 45 Cell size | 2 - hourly culture 3-6xt, 2 microns. The cells have the appearance of rods, which after 16-30 hours turn into clostridia, having the form of cigars, and after 48-80 hours they sporulate. At a young age, the aste sticks are very mobile, the peritrichs are Gram stained. In the clostridial stage, they are also mobile, the prospore is seen in the cells and granulosis accumulates. .55

On agar medium grows only under anaerobic conditions, obra: at the colony of orange color, it is convex.

8 .2

with smooth edges, with a darker dotted center ingrown into the agar, a colony diameter of 2-6 mm.

In a liquid nutrient medium at optimum temperature and pH, the full development cycle takes place and actively ferments starch and pectin substances with the formation of gas (in% in total) of hydrogen 54-60, carbon dioxide 40-46.

Physiological and biochemical signs. Milk with litmus, decanted, acidified, forms gas, gelatin is actively diluted, cellulose decomposes under anaerobic conditions in the presence of starting doses of carbohydrates. Anaerobe. Optimum temperature for the development of 35-37 C

The optimal values are 4-7.2.

Attitude to Carbon Sources. It grows in environments with different carbohydrates, acidic medium and forms a gas. Xylose, glucose, lactose, sucrose, galactose, fructose, arabinose, maltose, dextrin, starch, pectin are actively used; does not use raffinose, does not digest spirits.

Relation to nitrogen sources. Weakly use ammonium salts and develop well on media with organic forms of nitrogen, such as casein, peptone, corn extract, amino acids (cysteine, cystine, aspartic and glutamine, histidine tyrosine, arginine).

Signs of the strain are resistant, the strain is not pathogenic for humans and animals

The Clodtridium felsineum 84 strain is stored in the museum of the All-Union Collection of Microorganisms of the Institute of Biochemistry and Physiology of Microorganisms, DN of the USSR, and has a collection number VKM B 1527D.,

The essence of the invention is that, when grown, the strain Clostridium felsineum 84 is capable of forming spores with an active complex of pectolytic enzymes that play a significant role in the flax lobe process.

The bacterial spore preparation is obtained by growing Clostridiura felsineum 84 strain on a nutrient medium of the following composition, g / l; potato or corn starch 15-22; corn flour 5-7; corn extract 4-5; chalk 4-6; ammonium sulfate 1; 50% ferric chloride solution 3 ml; pH of 5.8-7.4.

After digestion in 1 ml of the culture fluid, 200 to 280 million spores are contained.

In tab. t presents a comparative description of the dispute between the proposed strain and the known one, from which it is clear that the new strain forms spores with good survival during the same year, as well as vybkoy pectolytic enzyme activity, which is much higher than the known one: 1.9 times in pectinmethylexterase, 1.3-1.8 times, pectin transeliminase 1.7-1.9 times, polygalacturonate transeliminae 2-3 times; and at the same time it forms 3.5 times more spores, which accordingly increases the yield of the target product.

Table 1

The effectiveness of the drug pectolitin derived from culture

,

2, In the regenerated night liquor pH 6.8-7.0

Clostridium felsineum 84, on the process of the flax lobe reflecting the table. 2

ITABLE2

52 T 1.3

Disputes C1 introduced. felsineum 13 Disputes C1 introduced, felsineum 84

Note. 1 billion dispute / g - the titer of the drug.

The proposed method differs from the prototype strain in that by forming a more active complex of pectolytic enzymes, it shortens the flax lobe process compared to the prototype strain in the normal heat lobe by 9 hours (by 27 hours compared to the control) and in the loin in the regenerated liquid for 10 hours, while the strain prototype in the latter case is generally ineffective. Thus, the pre-continuation of table 2

50 5 1.2

4 20

A2 I, 3

The clostridium felsineimi 84 lagged strain allows to obtain a good quality flax lotion preparation due to Balanced synthesis of pectolytic enzymes.

. The expected economic effect from the use of the drug made on the proposed Clostridium felsineum 84 strain may be 5-10 rubles. per 1 ton of flax straw or 10-20 rubles. per 1 kg of the drug.

Claims (1)

The bacterial strain Clostridium felsineum 84 (All-Union Collection of Microorganisms of the Institute of Biochemistry and Physiology of Microorganisms of the Academy of Sciences of the USSR, collection number VKM B 1527D) used for flaxing.