SU1149303A1 - Process for simulating chronic abdominal ischaemia - Google Patents

Abstract

A METHOD FOR MODELING CHRONIC ABDOMINAL ISCHEMIA by gradually occluding visceral arteries, characterized in that, in order to increase the reproducibility of the method, a catheter is delivered to the orifice of the visceral arteries and after 7-10 days after surgery cotton oil is introduced 1-2 ml 2-3 times every other day within 50-60 days.

Description

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The invention relates to experimental medicine, in particular to models in medicine, and can be used to study the morphological and functional state of the digestive organs in the dynamics of chronic disorders of visceral circulation.

The aim of the invention is to increase the reproducibility of the method.

The procedure is carried out as follows.

A test animal, a dog, is taken, premedication is carried out (dimedrol 1% - 1.0 + fentanyl 0.005% - 0.5 + droperidol 0.0025% -, 0) and transferred to artificial respiration using small doses of muscle relaxants under general intubation anesthesia (hexetyl + nitrous oxide). Over the course of 16-18 cm, an upper middle laparotomy was performed. The spleen and loops of the fine squeeze, which are covered with a damp napkin, are brought into the wound. The projection of the visceral arteries at the level of ThXI-III opens the posterior leaflet of the fracture and isolates the aorta with visceral arteries. Depending on the objective of the experiment, a thin catheter is brought to the mouth of the target artery (celiac, cranial or caudal mesenteric artery), the second end of which is brought to the side wall of the body with the help of a trocar. To prevent displacement, the catheter is additionally fixed with a catgut to the back sheet. If it is necessary to simultaneously narrow the celiac and caudal mesenteric arteries, a catheter is inserted between them. After removing the end of the catheter to the outside, the operative wound is elapsed tightly in layers.

7–10 days after the operation (depending on the condition of the experimental animal), when the changes due to the operative trauma disappear, I – 2 ml of sterile cottonseed oil is introduced into the catheter, which causes the development of reactive inflammation leading to an increase in connective tissue. tissue and narrowing of the visceral arteries in the mouth. In total, 2-3 injections of vegetable oil are made every other day. The day after the last oil injection, the catheter is carefully removed (the fixing ketkut sutures are resolved by this time).

With a single injection or introduction of less than 1 ml of oil, the developing sclerotic process is not very pronounced, and the introduction of more than 2 ml of oil can lead to disruption of the normal arrangement of vessels, nerve plexuses and other small retroperitoneal spaces. Long-term presence of the catheter in the retroperitoneal space with four or five times the introduction of vegetable oil

there is a risk of infection and the formation of pressure sores in the vessels.

The experiments showed that 2-3 times the introduction of 1-2 ml of vegetable oil leads to stenosis of the arteries on day 0-14, after the last injection, and occlusion occurs at the end of 2 months (50-60 days).

Example 1. A mongrel dog weighing 18 kg under general intubation anesthesia (hexanal 1% - 36.0 nitrous oxide) with a slit in the right hypochondrium parallel to the costal arch up to 10 cm in length is broken into the cavity of the cavity, the wound of the stomach is drawn onto the cardiac and prepiloric cue the divisions of the latter are subserously superimposed by two looped electrodes. The free ends of the electrodes are held under the skin and fixed. Laparotomic wound sutured in layers tightly.

On the 15th day after the operation, a catheter was brought to the trunk of the celiac artery under sedation conditions. Under the general controlled intubation anesthesia, an upper median laparotomic incision. With the intersection of a part of the sternum open

5 cavity. The spleen and delicate intestine are brought into the laparotomic wound, and the stomach with the electrodes is retracted upwards and to the right. At the level of Th XI - LI, the posterior peritoneal sheet was opened and the celiac artery was mobilized over 4 cm. A thin rubber cord was placed at the mouth of the vessel. Surgical wound layers crammed tightly.

5 10 days after the operation, 2 ml was introduced into the catheter 2 times 2 times a day for 50 days of sterile cotton oil (diameter of the artery is 4 mm).

Histological examination fine

In these periods, the dusters showed that dystrophic changes were beginning to develop in the small intestine, in particular, necrosis of the villi, swelling of the mucous membrane crypts, and swelling of the submucosal layer were observed.

5 The proposed method is reproduced in 36 dogs. To compare the proposed method with the known in 36 healthy mongrel dogs, the cranial mesenteric artery was also gradually occluded using cellophane strips.

0 Analysis of the results of animal release after surgery showed that mortality with a known method after occlusion with cellophane strips was 41% (7 of 2 dogs), and after reproducing chronic abdominal ischemia by the proposed method, 8%, i.e. The proposed method allows to reduce postoperative lethality five times.

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Thus, in a chronic experiment of the visceral branches, abdominal aorment seems possible. The proposed method, in comparison with the study of chronic abdominal ish-known, can significantly reduce

mission, which is close to the development of postoperative animal mortality

ischemic lesions of the digestive organs-5 to increase the output of animals in the main screen, observed during occlusive time-experiment.

1149303

Claims (1)

METHOD FOR MODELING CHRONIC ABDOMINAL ISCHEMIA by gradual occlusion of the visceral arteries, characterized in that, in order to increase the reproducibility of the method, a catheter is brought to the mouth of the visceral arteries and 1–2 ml of cotton is injected 2-3 times 2-3 times after surgery. day for 50-60 days.