SU1082362A1 - Method of preparing biological implant - Google Patents

Abstract

CnOCdB PREPARATION OF THE BIOLOGICAL IMPLANT by picking up the bones of the fetus followed by holding it in a preservative solution, characterized in that, in order to obtain implants with low antigenic properties, the tubular bones of the fetus of the Astrakhan sheep are taken and kept in place and maintained by a toothbrush. formalin, further purified from brain tissue, periosteum and cartilage, then the grafts are placed in a fresh 0.5% formalin solution, after which the bones are transferred to a 25% honey solution prepared with a 0.25% formalin solution , and store them until surgery.

Description

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Ltd. Oi bO 1 The invention relates to medicine, namely, to transplant surgery and methods for preparing and preserving tissues. A known method of preparing a biological implant from the bones of a human corpse, followed by preservation of G1. There is also known a method of preparing a biological implant from the bones of the fetus of cows, followed by keeping it in a preservative solution 121. However, the known methods do not allow to obtain a biological implant with low antigenic properties. implants with low antigenic properties. This goal is achieved in that according to the method of preparing a biological implant, which includes taking the fetal bones and then keeping it in a preservative solution, collects the tubular bones of the fetus of astrakhan sheep and holds them in a 0.7% formalin solution, then cleans the brain tissue , the periosteum and cartilage, then the grafts are placed in a fresh 0.5% formalin solution, after which the bones are transferred to a 25% plant and honey prepared with a 0.2% formalin solution, and stored until surgery. . The method is carried out as follows. Every year, in the spring during the period of lambing of Karakul sheep and in the fall when 2J clavibix ewes are slaughtered, the nocue remove the skins from the newly born gn and the fetus of the slaughtered ewes remain unused carcasses, of which a lot of long tubular pieces are made in field conditions. bones with their subsequent sterilization and preservation in formalin solutions. Next for 10 days. The bones are removed from the cans with formalin in the refrigerator, cleaned from the periosteum jMO3 ha and carved, cut in length. After bacteriological control, the treated bones and carcass are placed separately into jars of a smaller capacity (3-5 l) with fresh 0.5% formalin solution, which are placed in refrigerators. After 10 days under sterile conditions, they are transferred to barks with a 25% solution of honey prepared in 0.2% formalin. In this condition, at a temperature of (+2) - (- 4), bones are stored for up to 6 months with monthly bacteriological control and at any time can be used for transplantation with bone defects replaced and with osteosynthesis of fractures to enhance osteogenesis. The proposed method allows to obtain implants with low antigenic properties to obtain a cheap biological bone implant with high ductility and regenerative activity, practically in unlimited quantities in field conditions and to maintain their high plastic properties for a long time.

Claims (1)

METHOD FOR PREPARING A BIOLOGICAL IMPLANT by taking fetal bones and then maintaining them in a preservative solution, characterized in that, in order to obtain implants with low antigenic properties, the tubular bones of the fetus of astrakhan sheep are collected and kept at 0.7%. formalin solution, then cleaned of brain tissue, periosteum and cartilage, then the grafts are placed in a fresh 0.5% formalin solution, after which the bones are transferred to a 25% honey solution prepared in a 0.25% formalin solution, and xp Nhat to their operation. QO yo OS σ> to 1052 (62