SU1063834A1 - Strain of fungus cladosporium cladosporides 396 producing milenine pigment - Google Patents

Abstract

The strain of the fungus Cladosporium cladosporioides 396 (Collections of the Central Museum of Industrial Microorganisms VNIIGenetics, collection number TsMPM G-162) - producing melanin pigment.

Description

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The invention relates to the microbiological industry and concerns a new strain of the fungus used to bind heavy metal ions.

The biomass of the microscopic fungus or the melacine derived from it, the pigment is used for direct treatment of wastewater;

Melanin pigments of animal origin, inaccessible for industrial production, adsorb metal ions, and these abilities are not inferior to expensive synthetic ion-exchange materials lajm ij and 2,.

Known fungi of the genus Cladosporium form a melanin pigment, available for -culture in the microbiological industry. H.

However, the known fungi are not practical,

Cel5) of the invention is the preparation of a strain of Cladosporium dado spor i oi des 396 with the ability to bind heavy metal ions, which can be used in the production of

Morphological signs. The fungus colonies on wort agar are velvety olives-green, on the reverse side, dark brown to black, on „апapek’s agar medium, the colonies grow less intensively, the coloration of the colonies is burning. Conidiophores up to 400 "3.04,: mg, smooth, carry branching chains of conidia. Conidia are single-celled, slightly greenish, in branched chains, smooth, oval, elongated but blunt to almost spherical; apical conidia 2.2-3.5 2.03, 3 rw; median and basal 6.511, 4 -3.2-3.8 microns.

The fungus is an aerobic, the optimum growth and sporulation, the optimum value of water activity is 0.98, the pH is optimal pH 4-5, highly resistant to total doses of UV and UV radiation, artificial, high intensity sunlight. The fungus absorbs nitrate forms of nitrogen, uses glucose, sucrose, starch, pectin, cellulose, lemon, oxalic, acetic acid as the only carbon source, and has cellulolytic and tyrazinase activity.

The strain of the fungus is non-toxic, easily cultured under laboratory conditions.

For the production of fungal biomass under conditions of submerged cultivation, a modified čapek medium is used, in which ici of corn extract is added and sugar is used instead of sucrose. The cost per liter of medium is 3.6 kopecks.

The biomass formed during the growth of the fungus is separated by filtration from the culture liquid and dried at 6-0 ° C. .

The yield of fungal biomass is 15-20 g / l.

The strain of the fungus forms a melanin pigment of the indole nature, which belongs to the group of high molecular weight substances of irregular structure and has phenolic, quinoid, carboxyl and other functional groups. The pigment contains,%: carbon 58.2, hydrogen 3.6 and nitrogen 9.72, and is close to animal melanin. In a fungal cell, the pigment forms a complex with protein, which is 30% relative to the biomass of the fungus. The pigment dissolves in sodium hydroxide and concentrated H-5CU and HCf, does not dissolve in tetrahydrofuran, dry pyridine, chloroethane, butanol, ethanol, diethyl ether, chloroform acetone, and methanol, decolorizes with H-O. bromine water, KMiO4, gives a positive reaction with ammonia silver ions. Spectroscopy: absorbs UV, visible and IR light indiscriminately in the range from 200 to, 9t) Q them. A high concentration of paramagnetic centers of the order IlO PP / g of dry matter was detected on the electrode device of the electromagnet resonance. .

. The melanyl pigment is extracted from the fungal biomass of the POA by the modified method by hydrolyzing its non-melanic components by boiling it in a mixture of acetic and rosal acids. Tamm deposited in VNIgenetica, collection, number CMPM G-162.

Pigment production from biomass of the fungus ...

Example. A mixture of 100 g of dry powdered biomass of the fungus C. criadosporioides, 530 ml of ice-cold cassic acid, 330 ml of concentrated hydrochloric acid and 130 ml is boiled under reflux for 4 hours. The hot mixture is filtered and the residue is washed hot 80% acetic acid, then hot water and dried in air at., at the same time 36 g of melanin pigment are obtained from 100 g of biomass in the form of a black-brown powder ..

It has been established that during adsorption of metal ions, not only melanin isolated from the fungus can be used, but also the biomass of the species-producer itself (which is required 2.5-3.0 times more), since melane granules are located in the outer layer of the cell wall and are available for interaction with metal ions.

. Example. Extraction of metal from solution by melanin.

Melanin (1 g) or chopped dried fungus biomass is stirred for 3 hours with 1 l of solution; containing 0.001 M Pb (cibCOO) i.e. A., 32 g Pb (.j. The suspension is filtered and the lead content in the mother liquor is determined. The amount of lead adsorbed by melanin is determined by the difference in the content of lead ions in the solution before and after adsorption.

Extraction of ions of other metals presented in the table., Carried out in a similar way ..

As follows from the table, the resulting melanin by the ability to adsorb the ions of these metals is not inferior to animal melanin lj.

The maximum capacity of melanin (weight percent of the adsorbed metal in relation to the weight of melanin

reaches 40% upon mercury adsorption from a neutral Hg () solution; 30% from a solution of Nb (cN, COO) 2 in. 0; 17 M. 1% with adsorption. lead from solution Pb () j in

0.17 m.

Regeneration of the sorbent (in the case of 2c., Carried out by soaking the pigment with 20% hydrochloric acid for 5 hours) in the case of mercury -.

washing in a 10% aqueous solution of thiosulfate sodium for 5 hours.

  Thus, the melanin-containing biomass of the fungus or the melanin pigment is used to purify water and heavy metal ions, while due to the localization of the melanin pigment in the cell wall of the fungus, it is possible to use the most fungal biomass itself, while the melanin pigment adsorbs the Lead ions from the Suitable, solutions, , zinc, chromate, palladium thorium, latency and mercury.

Claims (1)

<claim-text> <table border = "1"> <tbody> <tr> <td rowspan = "2"> g / l </ td> <td colspan = "2"> 1. </ Td> <td colspan = "2"> 2 </ td> <td colspan = "2"> 3 </ td> <td colspan = "2"> 10 </ td> </ tr> <tr> <td> a </ td> <td> G "</ td> <td> ’I </ td> <td> b </ td> <td> a </ td> <td> 1 "</ td> <td> a </ td> <td> 1 ‘</ td> </ tr> <tr> <td> Pb (CH <sub> 3 </ sub> COO) <sub> g </ sub> 0.32 in water ’</ td> <td> 37 </ td> <td> 80 </ td> <td> </ td> <td> - </ td> <td> 75 </ td> <td> <sup> 52 </ sup> </ td> <td> .95. </ Td> <td> 20 </ td> </ tr> <tr> <td> Ma <sub> r </ sub> Pb.C14 0.30 to 0.01 'Cnc1c > 4 </ td> <td> 66 </ td> <td> <sup> 70 </ sup> </ td> <td> - </ td> <td> - </ td> <td> 95 </ td> <td> 50 </ td> <td> 100 </ td> <td> 10.6 </ td> </ tr> <tr> <td> Tb (ΝΟ <sub> λ </ sub>) <sub> 4 </ sub> 0.48 to 0.01 P1GST04 </ td> <td> 7. </ Td> <td> 16 </ td> <td> - </ td> <td> - * </ td> <td> 17 </ td> <td> 13 </ td> <td> 68 </ td> <td> 16 </ td> </ tr> <tr> <td> Ba (CH <sub> 3 </ sub> COO) <sub>? </ Sub> 0.33. in water </ td> <td> 39 </ td> <td> 60 </ td> <td> - </ td> <td> - </ td> <td> 68 </ td> <td> 33 </ td> <td> 91 </ td> <td> 13 </ td> </ tr> <tr> <td> 1p (CH-} C00,) <sub> 7 </ sub> · 2H <sub> 2 </ sub> O 0,0028 in water </ td> <td> .95 </ td> <td colspan = "2"> 0.76 95 </ td> <td> 0.76 · '</ td> <td> 100 </ td> <td> 0.3 </ td> <td> 100 '</ td> <td> 0.8 </ td> </ tr> <tr> <td> Bn (CH <sub> 3 </ sub> COO) <sub> 2 </ sub> · 2H <sub> g </ sub> O 0 ', 4 3 in water </ td> <td> - </ td> <td> - '</ td> <td> 60-70 * </ td> <td> 4-5 </ td> <td> - </ td> <td> - </ td> <td> - </ td> <td> - </ td> </ tr> <tr> <td> CgOz 0.02 in water </ td> <td> 94 </ td> <td> 9.4 </ td> <td>  '- · "</ td> <td> </ td> <td> 9? </ Td> <td> 3.2. </ Td> <td> 100 </ td> <td> 1.0 </ td> </ tr> <tr> <td> 0050.15. enter </ td> <td> - </ td> <td> - ·. </ Td> <td> 100 </ td> <td> 40 </ td> <td> - </ td> <td> - </ td> <td> - </ td> <td> - </ td> </ tr> <tr> <td> СЮ3 0.55 in water </ td> <td> - </ td> <td> </ td> <td> 70-90 </ td> <td> 100130 </ td> <td> </ td> <td> </ td> <td> </ td> <td> </ td> </ tr> <tr> <td> H £ (CH <sub> 3 </ sub> COO) <sub> 2 </ sub> 0.32 in the input </ td> <td> 90 </ td> <td> 180 </ td> <td> - </ td> <td> </ td> <td> - </ td> <td> -. </ td> <td> - </ td> <td> - </ td> </ tr> </ tbody> </ table> <claim-text> Note - Depending on mixing time} the amount of adsorbed metal: a - to the initial concentration,%, b - per 1 g of melanin, mg. </ claim-text>