SU1048382A1 - Qualidil qualitative determination method - Google Patents

Abstract

METHOD QUALITATIVE DETERMINATION OF KYALIDIL using a sample / treatment of an analyte with a reagent to obtain a colored compound, that is, in order to increase the sensitivity of the method, the sample is ana-. lysing follower is treated with bromophenol blue: in an alkaline medium, chloroform, and a solution of hydrochloric acid to form a r-colored layer of chloroform.

Description

ABOUT

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The invention relates to methods for the qualitative analysis of pharmaceutical preparations and can be used in pharmacies, control analytical and factory laboratories, in the forensic chemical examination

Qualidyl 1, b-bis-3 (3-benzyl-quinuclidyl-1) -hexane dichloride is a potent muscle relaxant with a curare-like effect.

A known method for the qualitative determination of qualification by treating a sample of an analyte with chromotropic acid disodium salt to form colored compound i.

The disadvantage of this method is the relatively low sensitivity (80-100 µg / ml).

The closest to the proposed technical essence and the achieved results is the method of determining qualification by treating a sample of the analyte with a solution of the ammonium salt of Reineke to obtain a colored compound 2. . .

The disadvantage of this method is relatively low, feeling BHTejibHOCTb (80-10Q µg / ml).

The purpose of the invention is to increase the sensitivity of the method.

The goal is achieved by the fact that according to the method of determining qualification. A sample of the analyte is sequentially treated with industrial phenol blue in an alkaline medium, chloroform, and hydrochloric acid solution to form a colored layer of chloroform.

The sequential implementation of the described stages constitutes a distinctive feature of the method.

The condition for the reaction is the simultaneous existence in ionized form of both the dye and the organic base, which is not always possible. If such an interval H exists, then it is bound to be in a weakly acidic or close to neutral medium, where the dye is, in the form of one

charge anion colored yellow. As a result, the formed ionic associates also have the same yellow color (Sjux 400415nm) in rare cases (crimson C / lacquer - 570-580 nm), which in reaction with bromophenol blue (BF) levels the properties of organic bases and makes it impossible to identify them.

 and me. In a maleic test tube with a ground up stopper., 2-3 mg of the preparation, 1 ml of 0.1 M NaOH solution, 4 drops (LO, 2 ml) of a 0.04% 6FS solution and 1 ml of chloroform. 5 The contents of the tube are shaken for 30 seconds and a blue color of the chloroform layer is observed. Then, drop by drop, after shaking the tube after each addition, add 1 ml of 0.1 M YSb solution. At e5: chloroform gets first green and then yellow.

When analyzing the qualification in the injection solution (2-5%), it is enough to add 1-2 drops of the test liquid to the tube and then, similarly to that described,

To characterize the sensitivity of the reaction, the molar absorption coefficient of the associate with respect to a 0.0003 M solution qualifier (fj iaKC- 5 14 X 10) was determined. The outcome of Sedela's sensitivity coefficients Ng 0, 012 and the coefficients of Koch and Koch-DeDitz Vx -0.6 are calculated from yakS P; The minimum openable concentration is Cmin 0., 75 µg / ml.

None of the quinuclidine group preparations (aceclidine, oxylidine, temechin, imi n, phencarol, quinine, quinidine), as well as the products of their synthesis (3-hydroxyinuclidine, quinuclidon-3), give a similar reaction.

Comparison of data of the proposed method with those described in the regulatory documentation presented in the table.

The proposed method allows to increase the sensitivity of the method 10 times.

Claims (1)

METHOD. OF QUALITATIVE DETERMINATION OF QUALIDYL using the processing of a sample of an analyte with a reagent to obtain a colored compound, which is possible in that, in order to increase / sensitivity of the method, the sample is ana. the lysed substance is sequentially treated with bromophenol blue in an alkaline medium, chloroform, and a solution of hydrochloric acid to obtain a colored layer of chloroform. □ About X)> 10.48382