SK286998B6 - Full artificial animal and/or poultry feed concentrate - Google Patents

Abstract

Full artificial feed concentrate for animal and/or poultry, that is applicable separately and/or as an additive to conventional feed containing proteins, sugar, polysaccharides, lipids, nontoxic compounds of biogenous elements and water, whereby the said feed concentrate contains beta-D-glucan in an amount from 0.1 to 9.0% by weight of dry residue, at least one nontoxic aliphatic diol to nonanol (e. g. glycerol, propylene glycol, di-glycerol, xylitol, sorbitol, maltitol) in an amount from 0.1 to 99.9% by weight or a mixture thereof with solid carrier and/or adjuvant (e. g. vitamins, amino essential acids, compounds of biogenous elements).

Description

Technical field

The invention relates to a complex animal feed and / or poultry fortification based on technically relatively readily available, mainly natural, ecologically acceptable raw materials, not only with a nutritional, but mainly energy-therapeutic, especially immunostimulatory effect.

BACKGROUND OF THE INVENTION

They have long been known as carbohydrates or energy feed components of polygastric and monogastric animals, respectively. carbohydrates, in particular glucose, sucrose and starch, alcohols, in particular ethanol or even aliphatic carboxylic acids and lipids [Bergner H., Sommer A .: Árch. Anim. Nutr. 46, 217-236 (1994); Hoffinann L .; ibid., 237-254; Sommer A: Biologization and Chemization of Animal Nutrition, no. 1, 65 (1976)]. Also known is the use of propylene glycol and glycerol as an energy feed donor, and at the same time an effective complement to the prevention of animal ketosis [Griffiths (1952), Johnson (1954); Cuboň J. et al .: Animal. Art. 39 (7), 643 (1994); Bergner H. at. al: Ar. Anim. Nutr. 48, 245 (1995); SK 283 287; UV CZ 8595; Their energy benefit in nutrition is evident, but therapeutic is only effective against the disease of ketosis of polygastric animals. Also known is bb-1,3 / 1,6-D-glucan, respectively. beta-glucans (β-D-glucan), which have a significant effect on the functioning of the immune system of humans and apparently also animals, manifesting themselves in better defense against viruses, parasites, bacteria and aberrant cell growth [Wasser SP, Weis AL: Crit. Rev. Immunol. 19 (1): 65-96 (1999); Ohno, N. et al., Biol. Pharm. Bull. 24 (7), 820-828 (2001)], even in the prevention and treatment of cancer. In addition to the immunomodulatory effects β-glucan lowers cholesterol, helps in the treatment of high blood pressure. Its effect on the growth of probiotic strains of bacteria in the intestinal tract is known [Jaskari J. et al .: Appl. Microbiol. Biotechnol. 49, 175-181 (1998)] and improves blood lipid profile [Nicolosi R. et al .: Am. J. Clin. Nutr. 70, 208-212 (1999)]. Thus, β-glucans belong to a group of physiologically active substances, collectively commonly referred to as biological response modifiers. Thus, basically any substance that stimulates an increase in the number or activity of cells of the immune system, production of mediators (cytokines), decreases suppressor mechanisms, increases the body's defense by being an effector or mediator, or increases the host's tolerance to cytotoxic damage [Palisa V., Holan Z .: Pract. Medicine. 69, 770 (1989); Novák M .: ICT Prague (2006)]. Thus, β-D-glucan has attracted attention not only for food, pharmacy and medicine producers [Novák M .: ICT Prague, β-Glucan, History and Present (2006); Patents: SK 285 062; SK 282 870; SK 274 918; US 6,476,003], in particular by its immunostimulatory effects or immunostimulatory effects on the human organism, but also on animals [Hahn T. W. et al .: J. Anim. Sci. 84, 1428 (2006); Mao, X.F. et al., J. Anim. Sci. 83, 2775-2782 (2005); Dritz S.S. et al: J. Anim. Sci. 73, (12), 3341-3350 (1995); Eicher S.

D. et al., J. Anim. Sci. 84 (9) 2352-2360 (2006)]. The effect of both β-D-glucan and antibiotics on pigs and β-glucan supplements on non-specific immunity is evaluated. True, β-D-glucan is usually also applied with drugs such as antibiotics and the like. There is even an attempt to use barley, which is also a donor of glucans in broiler fattening, which in turn increases and decreases due to better nutrition [Eila N. et al .: Tagung Schweine und Geflúgelemährung, Univ. Halle - Wittenberg (28-30 November 2006)].

SUMMARY OF THE INVENTION

The present invention is based on a complex animal and / or poultry fortified feedstuff, applicable as appropriate to fortification alone and / or as a feed component according to the intended application, wherein the feeds are based on known components such as proteins, carbohydrates, polysaccharides, lipids, atoxic salts, atoxic compounds. % of macroelements, amino acids, water and excipients, calculated as dry matter, comprise from 0.1 to 9% by weight. 0,2-D-glucan and from 0.2 to 90% by weight, of at least one aliphatic diol to nonanol, or together with a solid carrier and at least one excipient selected from vitamins, essential amino acids and bioelement compounds.

The advantage of the complex animal and / or poultry fortify complex according to the invention is the synergistic effect of the main components, also manifesting a therapeutic effect on animals and poultry.

At least one diol to nonanol or feed energy component together with B-D-glucan as an immunostimulatory component, not only allows to increase the performance and performance of animals and poultry, also due to the immunostimulatory effect of B-D-glucan and animal fitness mainly by energy donors. Another advantage is the raw material and technological availability of components of the complex fortified feed, their good shelf life and hygienic flawlessness.

At least one atoxic diol to nonanol is selected from propylene glycol, glycerol, diglycerol, xylolite, sorbitol, D-mannitol and glycerol, the so-called. glycerine phase as a byproduct of meta glycerides

2866 and / or ethanol. The glycerin phase according to the invention may contain alkali metal chlorides or phosphates, optionally also partially saponified glycerides and / or alkali salts of higher fatty acids. Also suitable according to the invention, but technically more difficult to obtain hexanol, is also lactose, even with 8 to 9 hydroxy groups such as lactose, maltose, isomaltose, 6-O-α-D-ghicopyranosyl-D-glucitol and others. The technically most suitable, however, is the so-called. glycerine phase from the production of methyl esters or ethyl esters of higher fatty acids. In the case of so-called. The glycerol phase from the production of higher fatty acid ethyl esters does not need to remove ethanol from the glycerol phase for the complex fortify not only for polygastric but also for monogastric animals and poultry.

As the solid carrier of the at least one diol to nonaol, it is selected from hydrophilic powder carriers such as natural and / or synthetic zeolites, silicates, silicalite, perlite, expanded perlite, oligosaccharides to polysaccharides containing at least 2 hydroxyl groups in the structural unit, such as starch, cereal flour, cereal meal, soy meal, maize meal, rapeseed meal and bran.

According to the invention, in particular, for monogastric animals and poultry, β-D-glucan of the most suitable purity of 90 to 99.9% by weight can be applied, but it can also be of substantially lower purity, but with lower efficiency. In the case of polygastric animals, on the other hand, B-D-glucan can also be used in the form of complexes with proteins, oligosaccharides to polysaccharides such as D-glucanic acid and hyaluronic acid and N-acetyl-D-glucamine and chitin / glucan complex. Thus, for polygastric animals a BD-glucan donor can also be uninsulated BD-glucan, even in native form, especially after treatment by purification, gentle drying and disintegration to a particle size of 1 to 300 µm, optionally impregnated, or in the form of gel to paste with at least one diol to organic compounds such as iron, zinc, cobalt, copper, iodine and selenium. Compounds of usually other macroelements, such as calcium, magnesium and phosphorus, may also be associated with them in justified cases.

Further detailed information on the complex fortify, as well as the benefits of its application, positive effects on the immune system of the animals, their condition, performance and performance, and the therapeutic effect at all, are also evident from the examples.

DETAILED DESCRIPTION OF THE INVENTION

Example 1

Re-esterification of rapeseed oil glycerides with methanol, catalyzed by sodium hydroxide, after separation of methyl esters of rapeseed acids (mainly oleic, stearic, palmitic, linoleic, linolenic, erucic and other unsaturated and saturated C16.22 monocarboxylic acid methyl esters), ₁₆ . _By separating the admixture of free carboxylic acids and the admixture of partially esterified glycerides, such as neutralizing alkali hydroxides and displacing alkali metal cations with trihydrogenphosphoric acid, it remains as the heavier bottom phase, the so-called. glycerine phase as a by-product. It is composed of (in wt.%): 82.2 Glycerol, 3.2 of methanol, 9.93 of water, orthophosphoric acid of 1.8, 2.6 sodium dihydrogen phosphate, 0.25 interesterified fat mixtures of partial glycerides, methyl esters of carboxylic acids, C ₆ -C C22 and lipids. After tracing the methanol to a content of 0.3 wt. and adding 0.04 g / dm ³ of cobalt chloride, 0.05 g / dm ³ of ferric chloride, 0.02 g / dm ³ of zinc chloride, 0.005 g / dm ³ of copper acetate, 0.003 g / dm ³ of selenium chloride, 4 g / dm ^{3 of} methionine, the obtained solution forms a complex fortify of feed no. First

Under otherwise similar conditions, fortification no. 2, except that a higher sodium hydroxide content with potassium hydroxide is used for the transesterification of rapeseed oil. Hydrochloric acid is applied instead of trihydrogenphosphoric acid to neutralize it after the esterification of the glycerides as well as the release of the higher fatty acids from their alkali salts. The glycerol phase obtained after removal of methanol has the composition (in% by weight): glycerol 80.2; water 13.1; sodium chloride 5.8; potassium chloride 0.2; methanol 0.2; the remainder being a mixture of predominantly transesterified lipids.

Sample no. 3 is a complex fortified feed based on glycerin phase, characterized in the preparation of the previous sample no. 2, but fortified in addition to 1.2 wt. β-D-glucan in powder form, with a purity of 99.8% by weight. and a particle size of 2.5 to 96 µm.

Sample of Complex Fortified Feed No. 4 is similarly formulated as sample no. 3, but additionally it is formulated with 0.04 g / dm cobalt chloride, 0.03 g / dm ferric chloride and 4 g / dm ³ DL-methionine.

In sample no. 5 in contrast to sample no. The β-D-glucan donor is a disintegrated mixture of the dried particles of the oyster mushroom (Pleurolus ostreatus), with an admixture of up to 5% by weight. % Tylopilus felleus and 2.5 wt. Sacchoomyces cerevisiae.

In sample no. 6 in contrast to sample no. 4 β-D-glucan donors are the disintegrated dried portions of oyster mushroom with otherwise equal amounts of β-D-glucan and other ingredients in the complex fortified animal and / or poultry feed, but is fortified in addition to DL-methionine and L-lysine hydrochloride (0, 5% by weight) and vitamins A, E, D ₃ , K ₃ , B _b B ₂ and B ₆ .

The above feed fortification formulations are tested on bovine Slovak spotted breeds with an average body weight of 404 to 420 kg, with reduced voracity, suspected of ketosis, resp. acidoketonémiou. During 3 weeks, twice daily application (morning and evening), in addition to the same amount of standard feed for each bull, after 300 g of complex fortified feed at 6 bulls, while 2 bulls do not receive any complex fortified feed. Next only once a day after 300 g of complex fortified feed. All bulls, except for vortex monitoring and general assessment (monitoring) of their health, shall be checked for urinary ketone content. All bulls had 1.5 to 3.5 mmol / dm ³ ketones in urine prior to measurement. The results are shown in Table no. 1. Remarkable is not only the effect itself, the so-called. but also the synergistic effect of glycerol with β-D-glucan. In virtually all bulls, no signs of ketosis or ketosis were present after 14 days of complex fortification, whereas ketosis was not deepened in bulls without fortification, and even in the third week of follow-up they showed signs of oral inflammation (Stomatitis) and skin (Dermatitis). But even after deployment to sick bull since the 21st day, when he began to give him a complex fortify feed no. 3 and drinking water after adding twice a day 300 g of complex fortified feed, after another 3 weeks no more ketones in the urine (the content of ketones determined with Pentaphenon comes out negative) and the symptoms and symptoms of oral and skin inflammation cease.

Table 1

mark Application Count Ketone content in bull urine [mmol / dm ³ ] remark fortification application (number) perigreeate daily (number) bulls Before applying the fortificate After 7 days After 14 days After 29 days - 0 1 1.50 3.50 7.50 13,00 1 1 1 3.50 1.5 1.00 neg. 2 1 1 3.50 1.00 neg. neg. 3 1 1 3.50 1.00 neg. neg. 4 1 1 3.50 neg. neg. neg 5 1 1 3.50 neg. neg. neg. 6 1 1 3.50 1.00 neg neg.

Example 2

Of the 24 bulls, 6 low-growth, with a ketone content of 3.5 to 7.5 mrnol / dm of ⁵ and 30 of the Gypsy breed sheep at the initial lactation stage of 8 animals with a symptom of ketosis (3.5 - 7.0 mmol) were selected. dm ³ ketones in urine) and with reduced feed intake. The composition of the feed ration of bulls is similar to that of Example 1 and in sheep weighing 40 to 45 kg / head, daily grazing consumption of 8 to 11 kg of grazing / head. By bull, 250 g / pc of complex fortified feed is added to the bull in the morning and evening as a sample of complex fortified product no. 5 in Example no. 1 and also in sheep, as samples of complex fortificate no. 5 in Example no. 1, twice a day at 40 cm ³ . The results obtained are shown in Tables. 2 and 3.

Table no. 2

Fortification application (number) Number of bulls [Ks] Ketone content in bull urine [mmol / dm ³ ] remark Before applying the fortificate After 7 days After 14 days After 21 days - 1 1.50 1.50 3.50 3.50 - 1 neg 1.50 3.50 3.50 5 2 3.50 3.50 1.50 neg. 5 2 7.50 3.50 neg. neg. 5 2 7.50 3.50 1.00 neg. 1 2 7.50 5.50 3.50 1.00 * * Forticate without β-Dglucan

SK 286998 Β6

Table no. 3

Application of fortificate (number) Number of sheep FCS] Ketone content in sheep urine [mmol / dm ³ ] remark Before application of fortificate After 7 days After 14 days After 21 days - 2 3.50 3.50 5.00 * 7.00 * * After 14 days 1 2 3.50 1.50 1.00 neg. in addition 5 2 3.50 1.00 neg. neg manifestations 5 2 7.00 3.50 1.50 neg. rarity and 6 2 3.50 1.00 neg. neg. milk fever

Example 3

Complex fortified fodder produced from the by-product of the production of unsaturated and saturated carboxylic acids methyl esters C ₁₆ to C ₂₂ catalysed by the transesterification of rapeseed oil with an aqueous solution of sodium hydroxide from so-called. glycerine phase. The glycerol phase after removal of methanol and fortification with methionine, cobalt acetate, iron (III) chloride, zinc chloride, lysine hydrochloride and a suspension disintegrated to a size of 40 to 52 μπ of lyophilized oyster mushroom containing 23.8 wt. β-D-glucan gives a complex fortified animal and poultry feed no. Ί of the following composition (in% by weight): glycerol 74.1; disintegrated oyster powder depths of 3.9; water 13.2; methanol 0.09; propylene glycol 2.5; methionine propionate 0.35; lysine hydrochloride 0.5; manganese chloride 0,01; cobalt chloride 0.03; ferric chloride 0.25; zinc chloride 0.154; Vitamin A 0.006; Vitamin E 0.005; sodium chloride 3.8 and a mixture of carboxylic acids C ₁₆ to C ₂₂ and lipids 1.1.

Of the 80 high-yielding dairy cows, 9 units are separated into two groups in the first lactation phase, while maintaining a comparable body weight of 660 to 720 kg / head.

Dairy cows were housed in two buildings with free standing, with a passage feed corridor and automatic drinkers. Feeding was carried out with a feeding wagon with mixing and dosing. In both groups, the same feeding rations were administered to the dairy cows in terms of both quantitative and qualitative nutritional value. But in dairy cows in group 1 (9 dairy cows), each dairy cow was given twice a day, in the morning and in the evening, with 330 g of complex fortificate feed no. 7. The second group was the control group. The dairy cattle liveliness in group 1 was approximately twice as high. The feed ration was consumed in a shorter time (up to 2/3 of group 2 time).

Over 90 days, the addition of complex feed fortificate 5.7 in group 1 was evaluated compared to group 2 of dairy cows fed without the addition of complex feed fortificate no. 7. The results of the mean and standard deviation of cows 1 and 2, checked at 10-30 day intervals, are summarized and compared in Table 2. 4th

In the second, control group, however, after 20 days, 2 dairy cows became ill, their voracity decreased significantly, they were indicated for inflammatory lung diseases, they were subjected to veterinary treatment. Thus, after 20 days, the results in Group 2 were used only from 7 dairy cows.

Table no. 4

parameter 1. odber (after 10 days) 2nd subscription (after 20 days) 3rd subscription (after 60 days) 4th subscription (after 90 days) Average daily milk yield [dm ³ / pc] First group Group 2 1st group Group 2 1st group Group 2 First group Group 2 24.4 19.8 24.9 20.1 24.42 14.25 22.9 16.8 Standard deviation fdm ³ / ksl 5.01 5.1 5.31 4.85 4.11 4.01 3.64 5.31 Minimum milk yield ³ mp / day and denl 12.5 5.9 14.1 5.1 14.0 6.5 13.9 5.9 The maximum milk yield is ³ / pc and denl 33 31 37 30 32 29 31 27 Difference in average milk yield 23.2 23.9 35.7 36.3

The results show an average higher milking rate of the dairy cows of the 1st group compared to the control 2nd group, in which two dairy cows were also infected. In addition to the increased milk yield of dairy cows of the 1st group, whose feed was fortified twice a day with complex fortification no. 7, its positive effect has been shown to be more aggressive in group 1 dairy cows, as well as in their very good physical condition, resulting in a shorter service period of 27 to 32 days, compared to dairy cows control and, last but not least, very good health.

Example 4

As the synergistic, energy-therapeutic to immunostimulatory effect of aliphatic di1010 hunting to nonanols is manifested along with BD-glucan or its donors, the positive effect on dairy cows with symptoms of ketosis has also been verified. Dairy cows, similar to Example 3, weighed 660-720 kg / pc. The complex fortificate in their feed was as follows (in wt.%): Glycerol = 59.5; propylene glycol = 3.2; sorbitol = 3.1; isomaltose = 1; xylitol propionate = 5.0; BD-glucan = 4.98; water = 15.2; methionine hydrochloride = 0.29; lysine hydrochloride = 0.55; manganese chloride = 0.02; cobalt chloride = 0.021; ferric chloride = 0.25; copper (I) chloride 15 = 0.05; zinc chloride = 0.121; calcium chloride = 1.23; sodium chloride = 4.4; the mixture of carboxylic acids, C ₆ to C ₂₂ and a lipid, 0.91; B-carotene = 0.10; vitamin A = 0.006; Vitamin E = 0.006; B-complex = 0.04; a mixture of vitamins K = 0.04 and sodium selenite = 0.02.

Complex fortificate no. 9 differed from complex fortification no. 8 only by replacing the B-D-glucan with an increased water content to a total content of 20.18% by weight. and instead of a mixture of glycerol, propylene glycol, 20 sorbitol and xylitol propionate, pure glycerol obtained from sunflower oil glycerides was used, the total content of which in feed fortification no. 9 constituted 71.8 wt.

20 dairy cows were selected from the dairy herd and the symptoms of ketosis became apparent. Of these, 12 dairy cows, in addition to feed and water content, received twice a day (in the morning and in the evening) of 375 cm ^{3 of} complex fortified feed no. 8 and 4 dairy cows fortificate no. 9, also twice a day at 375 cm ³ . The results obtained are shown in Table 1. 5th

Table no. 5

Fodder fortification application (number) Number of dairy cows [pcs] Ketone content in dairy cows [mmol / dm ³ ] Before applying the fortificate After 7 days After 21 days After 42 days inspection 2 1.5 1.5 3.5 5.0 inspection 1 3.0 3.5 5.0 7.5 inspection 1 3.0 3.5 5.0 7.0 8 4 1.5 neg. neg. neg. 8 4 3.0 1.5 neg. neg. 8 2 3.5 1.0 neg. neg. 8 2 3.5 1.5 neg. neg. 9 2 3.0 2.5 1.0 neg 9 1 3.5 2.5 l of neg. 9 1 1.5 1.0 neg. neg.

In 4 dairy cows not fortified with feed, not only did ketosis progressively deteriorate, but in two, symptoms also began to develop after 3 weeks and, after 40 days, mucosal inflammation began to occur and, of course, their liveliness and milk yields decreased significantly. The milking rate of the 'most productive' dairy cow was always calculated.

While for dairy cows consuming complex fortified feedstuffs no. 8, the milk yield increased by 11 to 14% on average after 7 days, by 16 to 20% after 21 days and by 26 to 52% after 42 days. In the case of dairy cows No. 9, fortified mainly by glycerol and trace element compounds, milk yield was 11 to 13% higher after 7 days, 16 to 18% after 21 days and 22 to 34% after 42 days. In the case of two other dairy cows, such as controls which received the same and quantitative feed as

4 dairy cows control, but twice daily received β-D-glucan in the same amount as 12 dairy cows with complex feed fortificate no. 8, but without additional components. They were given β-D-Glucan applied to the flour, ensuring complete consumption of β-D-glucan with the flour twice a day. One cow had 1.5 mmol / dm ³ ketones in the urine at the start of the experiment and the other 2.5 mmol / dm ³ . Already after 21 days the urinary ketone content of both dairy cows dropped to 1.0 mmol / dm ³ , they were more voracious than the control dairy cows and after 42 days in both dairy cows, in good health, no ketones were present in the urine. Flour as a carrier of β-D-glucan could also partially help.

However, the synergistic effect of β-D-glucan with diols to nonaols of the complex dairy fodder fortify is evident.

Example 5

The procedure is similar to that of Example 4, except that the intake of the complex feed fortificate is not carried out in the liquid phase in the presence of larger amounts of water, in the presence of glycerol, respectively. glycerol propylene glycol sorbitol xylitol propionate, but deposited or adsorbed on the perlite powder, the expanded perlite being less than 0.1 mm of this composition (in wt.%); SiO ₂ = 70.5; Al ₂ O ₃ = 13.5; TiO ₂ = 0.4; Fe ₂ O ₃ = 1.5; CaO = 1.3; MgO = 0.7; MnO = 0.3; K ₂ O = 3.1 Na ₂ O = 3.5; P ₂ O ₅ = 0.1 and H ₂ O = 5.0. This perlite after bonding of 50.1 wt. Compound feed fortificate composition (% by weight): 85.3 glycerol;

6.1 β-D-glucan; 5.4 micron milled dolomite CaCO ₃ .MgCO ₃ ; 0.15 sodium selenite; 0.35 mixtures of vitamins A, E, K; 2.2 NaCl and 0.5 NaH ₂ PO ₄ , after thorough mixing, had a free-flowing form. Complex fortification of feed No. 10 at 350 g twice a day for dairy cows for 8 cows with a ketone content of 1.0 to 1.5 mmol / dm ³ in two control dairy cows also with a starting ketone content of 1.0 to 1.5 mmol / dm ³ , all 8 dairy cows consuming the complex fortify twice daily for 7 days had no ketones in their urine and their milk yield was 10 to 15% higher than the two control dairy cows. After 42 days, they had already increased milk yield by 21 to 36% while, in addition, two control dairy cows measured urinary ketone levels of 3.5 to 5.0 mmol / dm ³ and markedly started to show quite lower gluttony as well as symptoms inflammation of oral mucous membranes.

Example 6

Complex Fortified Feed No. 10 consists of (wt%) 70.8 glycerol; 2.0 6-O-α-D-glucopyrazonyl-D-glucitol; 4,6 β-D-glucan; 1.17 methionine hydrochloride; 0.06 cobalt chloride; 0.24 ferric chloride; 0.18 zinc chloride; 0.35 calcium chloride; 0.25 sodium chloride; 0.04 cupric chloride;

5.8 diglycerol; 3.0 dibasic calcium phosphate; 10.2 water; 0.06 Vitamin A; 0.18 vitamin E; 0.09 Vitamin K ₃ ; 0.28 mixture of vitamins B and 0.5 of vitamin C.

It is applied as a feed component of racehorses (Arabian warm-blooded horse), of which 10 are applied to 6 horses by careful injection into the horse muzzle twice a day, morning and evening, 180 g each, otherwise sufficient quality standard horse feed. Smaller group - 4 horses, however, does not receive a complex fortified feed. The horse's weight was 690 to 730 kg. However, after a week's application to horse training, the performance of the horses receiving the complex fortified feed was 11 to 21% higher, and moreover, the horses of the controls after the difficult hurdle training were in most cases so tired that they received a shiver. After 2 weeks, two horses were also given skin inflammation (Dermatitis) and after 3 weeks they also received skin inflammation and a third horse. After being deployed after 4 weeks to the horses of the control group twice a day of complex fortified feed, for the next 2 to 4 weeks, the horses of the original control were also improved.

Example 7

Complex fortificate no. 11 is prepared by adsorbing to expanded powdered perlite to change 0.04 to 0.1 mm and a bulk density of 95 kg / m ^{3 with} glycerol, in an amount of 30% by weight. and intensively mixed still with β-D-glucan powder in an amount of 1.5 wt. and 5 wt. % barley meal with 10 wt. water. The initial expanded perlite used is of the following composition (in% by weight): SiO ₂ = 70.5; Al ₂ O ₃ = 13.5; TiO ₂ = 0.4; Fe ₂ O ₃ = 1.5; CaO = 1.3; MgO = 0.7; MnO = 0.3; K ₂ O = 3.1; Na ₂ O = 3.5; P ₂ O ₅ = 0.1 and H ₂ O = 5.0.

When administered to two groups of broilers of the COBB500 hybrid, the first group of 500 pieces at the age of 6 days is given, in the morning and in the evening, in addition to the same quantity and quality of feed as the second group, also 500 pieces after thorough mixing with other feed ( with sufficient water in both groups) twice daily for 25 g / pc of complex fortificate no. 11. Group 1 broilers grew to 37 carcasses in 37 days, but 6 were killed and 39 days in the 2nd control group, but up to 44 died.

Example 8

Complex fortified animal and poultry feed is prepared using so-called. glycerine phase as a byproduct of the production of the carboxylic acid ethyl esters of C ₁₆ to C ₂₂ from rapeseed oil. The glycerol phase has a composition (in% by weight): glycerol = 51.9; sodium chloride = 4.5; sodium dihydrogen phosphate = 2.5; ethanol = 3.0; a mixture of partially transesterified lipids, phospholipids, ethyl esters of carboxylic acids as well as free fatty acids = 2.5 and water 38.5.

Of the amount of 5,000 g, further 15.1 g BD-glucan, 0.5 zinc chloride, 0.9 g ferric acetate, 0.2 g manganese acetate, 0.15 g cobalt chloride, 1 g ascorbic acid (vitamin C) ), 0.5 g of a mixture of vitamins B, 0.3 g of vitamin A is formulated complex fortify feed no. 12, intended mainly for sows. In order to improve the health of the sows and, in particular, the number of births and viable piglets, 8 sows of comparable weight shall be selected and 300 g of complex fortificate added to the feed twice a day after thorough homogenization for 6 weeks of sowing. no. 12. Dosage of the complex fortificate continued until 6 weeks after the birth of the piglets. While 6 sows gave birth to 10 to 13 piglets, in 2 control sows it was 9 and 10 piglets. Despite the otherwise equally high-quality care of all 8 sows using fortificate no. 12, from 6 sows, 9 to 12 piglets were finally raised, in the control two it was only 7 piglets. Finally, mastitis also occurred in one sow.

To avoid a mistake in comparison, 6 sows were given a complex fortificate at the beginning of feed access, taking care of sufficient quantity and quality and satiety of all 8 sows so that the pig's condition and performance could not be affected by more food intake than in two control sows. sows.

Example 9

Complex fortificate no. 13 as opposed to fortificate no. 12 in Example 7 differs only by substituting glycerol with lactitol (15 wt%) and maltitol (15 wt%). It was applied in broiler nutrition. Broilers of the 1st group with complex fortificate to the carcass weight grew in 37 days, with 7 pieces dead and in the 2nd control group in 40 days, but up to 42 pieces died.

Industrial usability

The invention is useful in the manufacture of animal and poultry feed, in the manufacture of feed additives and feed supplements. Also when using the so-called. glycerine phase as a by-product in the production of methyl esters or ethyl esters of higher fatty acids by re-esterification of glycerides, in particular vegetable oils, in particular rapeseed oil with methanol and / or ethanol. Methyl esters as well as ethyl esters are a component of diesel fuel. biodiesel or other biofuels. Last but not least, in agriculture, especially in livestock production, for use as a complex fortified animal and poultry feed not only with a nutritional but mainly energy-therapeutic, especially immunostimulatory effect on animals and poultry.

Claims (12)

Complex fortified animal and / or poultry feed, in the form of and separately or as a feed component, wherein the feed is based on known components such as proteins, carbohydrates, polysaccharides, fats, atoxic salts, atoxic macroelement compounds, microelements, amino acids, water and auxiliary substances, characterized in that, calculated on dry weight, it contains 0.1 to 9 wt. B-D-glucan; 99.9 wt. at least one atoxic aliphatic diol to nonaol or a mixture thereof with at least one solid carrier and / or at least one excipient selected from vitamins, essential amino acids and bioelement compounds. Complex animal and / or poultry feed fortify according to claim 1, characterized in that the at least one atoxic diol to nonanol is selected from propylene glycol, glycerol, diglycerol, xylitol, xylose, sorbitol, maltizole and glycerol. glycerine phase, as a by-product of the esterification of glycerides with methanol and / or ethanol. Complex animal and / or poultry fortified feedstuff according to claims 1 and 2, characterized in that the at least one solid carrier is selected from hydrophilic powder carriers, such as natural and / or synthetic zeolites, silicates, silicate, perlite, expanded perlite, oligosaccharides to polysaccharides containing in the structural unit at least 2 hydroxyl groups, such as starch, cereal flour, cereal meal, soybean meal, corn meal, rapeseed meal and bran. Complex fortified animal and / or poultry feed according to claims 1 to 3, characterized in that the BD-glucan content, calculated on the dry matter, is 0.2 to 8% by weight, wherein the BD-glucan applied is % purity 90-99.9 wt. and / or in the case of a fortify intended for polygastric animals, it is also in the form of compounds with proteins, oligosaccharides to polysaccharides such as D-glucanic acid and hyaluronic acid and N-acetyl-D-glucosamine and chitin / glucan complexes. Complex animal and / or poultry fortified feedstuff according to claims 1 to 4, characterized in that the fortify intended for polygastric animals contains as BD-glucan donor, which is at least one native fungus containing free and / or bound BD- glucan, selected from Auricularia auriculajude, Agaricus bisporus, Aspergillus niger, Grifola frondosa, Schizophyleum commune, Tylopilus felleus, Amanita muscaria, Sclerotium glucanicum, Pleurotus ostreatus, Lentinus edodes, Sacharomyces cerevisiae and Por. Complex animal and / or poultry feed fortificate according to claims 1 to 5, characterized in that at least one excipient is selected from essential amino acids, vitamins A, B, E, K and C. Complex animal and / or poultry feed fortificate according to claims 1 to 6, characterized in that at least one excipient is selected from compounds, usually deficient organic elements such as iron, zinc, cobalt, copper, iodine and selenium. Complex animal and / or poultry feed fortificate according to claims 2 to 7, characterized in that, in the case of glycerol in the form of the glycerin phase, it is in the form of an aqueous solution together with an atoxic mineral salt or atoxic alkali metal salts and / or a partially saponified glycerides with up to alkaline salts of higher fatty acids. Complex animal and / or poultry feed fortificate according to claims 1 to 8, characterized in that the atoxic mineral alkali metal salts form or form alkali salts selected from sodium and / or potassium chlorides and phosphates. Complex animal and / or poultry feed fortificate according to claims 1 to 9, characterized in that it can be applied alone and / or as a component of at least one of an aqueous solution, an emulsion, a suspension as part of a drinking water and / or a powder. also with adsorbed diol to nonaol. Complex animal and / or poultry feed fortificate according to claims 1 to 10, characterized in that the diol and / or triol donor is propylene glycol and / or glycerol, so-called diol and / or glycerol. a glycerin phase, preferably on a solid mineral support. Complex animal and / or poultry feed fortificate according to claims 1 to 11, characterized in that the B-D-glucan donor, in particular for polygastric animals, is at least one native fungus according to claim 1. 5, or refined dehydrated and disintegrated to a particle size of 1 to 300 µm, and / or fed with at least one diol to nonaol.