SK12022U1 - Disinfectant mixture, method of disinfection of air and surfaces and use of disinfectant mixture - Google Patents

Abstract

The disinfectant mixture contains active ingredients and other ingredients, the active ingredients being N- (3-aminopropyl) -N-dodecylpropane-1,3-diamine, 2-phenoxyethanol and bis (persulphate) -bis (sulphate) pentapotassium and the other ingredients being isopropyl myristate α-hexylcinnamaldehyde, 4-tert-butylcyclohexyl acetate, eucalyptol, 1- (2,3,8,8-tetramethyl-1,2,3,4,5,6,7,8-octahydronaphthalen-2-yl) ethanone, limonene, linalol, resublimed iodine, ethyl alcohol and fragrance composition. The disinfectant mixture is intended for use as a biocidal product for the disinfection of indoor air and surfaces. A method of disinfecting air and surfaces in enclosed spaces using this disinfectant mixture is also described.

Description

SK 1-2022 U1

Field of technology

The technical solution concerns the disinfectant mixture, the method of disinfecting air and surfaces in enclosed spaces and the use of the disinfectant mixture as a biocidal product.

Prior art

Air pollution damages human health and the environment.

In Europe, emissions of many pollutants have been significantly reduced in recent decades, leading to improved air quality throughout the region. However, pollutant concentrations are still too high and air quality problems persist. A significant proportion of Europe's population lives in areas, especially in cities, where air quality standards are exceeded and ozone, nitrogen dioxide and particulate matter pollution pose serious health risks. Several countries have exceeded one or more of their 2010 emission limits for emissions of four important pollutants. Reducing air pollution therefore remains important.

Approximately 90% of the population of European cities is exposed to pollutants in concentrations higher than air quality levels considered harmful to health. The presence of fine particulate matter (PM2.5) in the air is expected to reduce life expectancy in the EU by more than eight months. Benzopyrene is a carcinogenic pollutant of growing concern, with concentrations in many urban areas above the thresholds set for the protection of human health, especially in Central and Eastern Europe.

Indoor air purification is currently addressed in several ways, one of which is air purifiers. With an air purifier, you can reduce the likelihood of getting ill with the flu or other airborne illness. With the air purifier, improvements in allergy and respiratory problems can be achieved, and mold growth is also eliminated. Thanks to the effective HEPA filter system, it is possible to reduce the number of allergens in the air, as well as the occurrence of viruses and bacteria, cigarette smoke and other types of odor. Commercially available air purifiers are equipped with functions such as antiseptic plastic filter with silver ions for coarse impurities, electrostatic filter for dust separation, cotton LTC catalytic filter, HIMOP formaldehyde removal filter, HEPA dust removal filter, active carbon filter, plasma generator, negative ions (anions), technology for electrostatic dust separation, air humidification. The disadvantage of air purifiers is the need to replace filters, which is quite economical.

Another method of air purification is provided by a germicidal radiator, which is designed to eliminate microbes, bacteria and viruses in the air or on various surfaces (carpets, tables, examination chairs, examination aids). It is often used in environments where biological materials are handled (such as pharmacy, laboratories, veterinary facilities, chemical and food industries).

We distinguish two types of germicidal radiators, namely: closed (this is indirect irradiation) or open (direct irradiation). When we talk about a closed type of radiator, it means that it is used for indirect irradiation of the room, which can be used in the presence of people. No chemicals are used with this type of radiator. It is a constant cleaning of the air, while the radiator has a source of UV radiation hidden in the tunnel through which the air flows.

Polluted air enters the device in the lower part and exits from the upper part, thanks to which a constant circulation of air in the room is ensured. The air is moved every 6 hours as a result of circulating in the room.

The second type of radiator is an open germicidal radiator. With this type of radiator, the room is disinfected without the presence of persons, due to the fact that direct radiation has a negative effect on the human body. It is best to sterilize the space in the evening, and by directing the radiator directly to a specific place or object. The recommended operating distance of the radiator is up to 1 meter, because then the effect of UV radiation occurs 100%. Radiation destroys microorganisms and their germs at the same time, ie in the air and on the surface at the same time. If microbes are to be removed, they must be exposed to UV radiation for a period of time.

Another known method of air purification is the ozone generator. This device is placed in the area you want to treat, and all windows and doors must be carefully closed when cleaning the air. It is advisable to connect the ozone generator via a timer, where the required treatment time is set. The ozone generator starts to generate ozone, and there are no people or animals in the room during this process. At least 40 minutes after the treatment time has elapsed before people can return to the room,

SK 1-2022 U1 or animals. The room must be properly ventilated after using the generator. The disadvantage of the device is that an unpleasant odor may persist in the room after its use. The biggest disadvantage of this solution remains that the ozone generator must not be used in the presence of humans, animals or plants.

The ozone generator does not replace washing, vacuuming, washing or beating (ie no mechanical cleaning). Therefore, in order for ozone to work properly, it is necessary to mechanically clean all impurities on the treated areas before using it.

The essence of the technical solution

The shortcomings of the hitherto known methods of disinfecting air and surfaces in enclosed spaces are solved by a disinfection mixture and a method of disinfecting air and surfaces in enclosed spaces, using this mixture, according to the present technical solution.

The disinfectant mixture contains active ingredients and other ingredients, the active ingredients being: N- (3-aminopropyl) -N-dodecylpropane-1,3-diamine at a concentration of 10,000 mg / kg 2-phenoxyethanol at a concentration of 5,000 mg / kg bis ( persulphate) bis (potassium) pentapotassium at a concentration of 5 000 mg / kg and the other ingredients are:

isopropyl myristate at a concentration of 902,000 mg / kg α-hexylcinnamaldehyde at a concentration of 10,000 mg / kg 4-tert-butylcyclohexyl acetate at a concentration of 10,000 mg / kg eucalyptol at a concentration of 10,000 mg / kg 1- (2,3,8,8-tetramethyl -1,2,3,4,5,6,7,8-octahydronaphthalen-2-yl) ethanone at a concentration of 10,000 mg / kg limonene at a concentration of 10,000 mg / kg linalol at a concentration of 10,000 mg / kg iodine resublimed in at a concentration of 99.98% 3,000 mg / kg ethyl alcohol at a concentration of 10,000 mg / kg fragrant composition at a concentration of 5,000 mg / kg.

Table 1 shows the composition of the disinfectant mixture.

Active substances

Chemical name EC number CAS number Concentration N- (3-aminopropyl) -N-dodecylpropane-1,3-diamine 219-145-8 2372-82-9 10,000 mg / kg 2-phenoxyethanol 204-589-7 122-99-6 5,000 mg / kg bis (persulphate) -bis (sulphate) pentapotassium 742-778-7 70693-62-8 5,000 mg / kg

Concentrations in metric units (eg 10 mg / l or 4.5 mg / kg)

Other substances

Chemical name EC number CAS number Concentration isopropyl myristate 203-751-4 110-27-0 902,000 mg / kg α-hexylcinnamaldehyde 202-983-3 101-86-0 10,000 mg / kg 4-tert-butylcyclohexyl acetate 250-954-9 32210-23-4 10,000 mg / kg eucalyptol 207-413-5 470-82-6 10,000 mg / kg 1- (2,3,8,8-tetramethyl-1,2,3,4,5,6,7,8-octahydro-naphthalen-2-yl) -ethanone 259-174-3 54464-57-2 10,000 mg / kg lemonade 227-813-5 5989-27-5 10,000 mg / kg linalol 201-134-4 78-70-6 10,000 mg / kg iodine resublimed 99.98% 231-442-4 7553-56-2 3,000 mg / kg ethyl alcohol 200-578-6 64-17-5 10,000 mg / kg fragrant composition does not have does not have 5,000 mg / kg

Concentrations in metric units (eg 10 mg / l or 4.5 mg / kg)

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All components are mixed at room temperature.

The disinfectant mixture is a clear oily liquid. The oil-based mixture does not irritate the respiratory tract.

This mixture disrupts the integrity of pathogens such as bacteria, fungi and viruses. It is suitable for day and night use indoors. It is not harmful to health and can be used in the room and in the presence of people. The results of the tests showed that the mixture functions as a biocidal product and has proven effects in purifying air from different strains of microorganisms.

The disinfectant mixture is a biocidal product that must be observed when working with safety precautions. The product must not be exposed to frost or direct sunlight.

The disinfectant mixture is used as a filler in diffusers, thus ensuring the disinfection of air and surfaces in enclosed spaces.

Air and surface disinfection is based on the application of domesticated biocidal oil to the interior using a diffuser. The basis is the cooperation of diffusers with a built-in atomizer and biocidal aromatized oils, which spread through the interior after darkening.

The undiluted disinfectant mixture is poured into the inner vessel of the diffuser and then the required exposure time and power intensity are set on the diffuser.

The atomized disinfectant mixture begins to spread into the interior, eliminating viruses and bacteria directly in the air.

The diffuser requires almost no maintenance, which saves time and operating personnel who would have to perform disinfection. Everything is done by the built-in control panel, which controls the setting of the action time and its intensity. You can change them at any time.

In practice, there are 4 main types of diffusers:

- nebulizing (spray)

- ultrasonic

heat, heating and

- evaporative (steam).

Each type of diffuser has a different way of releasing the oil-based disinfectant mixture into the space. It is possible to use any type of diffuser, each of which has its advantages and limitations.

The spray type diffuser disperses the oil into the space in the form of a fine mist through the pressure created by the diffuser mechanism. The disinfectant enters sublimation (a physical process in which a solid particle turns into a gaseous particle) and is thus able to disperse in space. The disinfectant released by the diffuser thus spreads into the space and spreads in a closed room due to the natural flow of air. The fine fragrance vapor created by the diffuser does not change the chemical composition because it does not heat the oil, only to eject it into space.

Advantages:

• the diffuser does not use heating, which achieves high disinfection efficiency • the diffuser does not use water, the oil does not drip into the water, but directly disperses into the space • the most effective way to get 100% of the disinfectant mixture into the space.

Ultrasonic diffuser - this type uses water into which you drip oil and thanks to the electric frequency, a fine mist of water vapor is created with oil molecules, which spreads around the room. The diffuser breaks down the oil into molecules that are negatively charged so that they can disperse by combining with positively charged molecules in space. The ultrasonic diffuser uses water as a form of oil transport in such a way that the ultrasonic vibrations act on the water, causing the oil molecules to be released and the negatively charged parts to disperse into the air together with the water vapor.

Advantages:

• low oil consumption, only water-soluble drops are used • no heating to ensure oil quality • humidifies the air by evaporating water (there may be a disadvantage for some)

Thermal diffuser - this type uses heat to allow the oil to evaporate into the room. Usually the oil drips back into the water, but this is not a condition, depending on the specific diffuser. The more heat is generated, the more oil evaporates. On the other hand, it is important to use as little heat as possible so as not to reduce the effects of the oil or change the properties of the oil.

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Advantages:

• lower price

Steam diffuser - this type uses a stream of air to disperse oil into the room. Drip the oil onto the filter (pad) inside the diffuser, without using water. A small fan blows air from the room into the diffuser through a filter on which the oil is applied, thus speeding up the evaporation of oil particles into the room.

Advantages:

• quiet, no heating, no water

Different types of diffusers can be used, but the maximum effect of the disinfectant mixture is achieved if the oil substance is not mixed with water or heated. Such a process is made possible by a diffuser with cold diffusion technology, which uses only the nebulizing method of oil filling distribution, so that the diffuser does not use water or heating.

The method of disinfecting air and surfaces in enclosed spaces using a disinfectant mixture works by inserting the disinfectant mixture into a diffuser. Before that, the disinfectant mixture is prepared in an amount determined according to the size and parameters of the diffuser vessel. The diffuser container is then opened and, if necessary, the safety catch of the closure, if the diffuser contains it, is removed.

The diffuser vessel is then filled with the undiluted disinfectant mixture and inserted into the diffuser head. The diffuser is set to the specified power intensity and time, which are set according to the size of the disinfected area and the degree of its contamination, while the exposure time is at least 1 hour. The diffuser is switched on via the diffuser control, then the disinfectant mixture is mixed with air by automatic diffusion through the diffuser head. After the set time has elapsed, the diffuser switches off automatically, with the diffuser optionally announcing that it has been deactivated by an audible signal, depending on the device type.

The diffuser can be of various types, but it ideally works on the basis of cold diffusion technology, which uses exclusively the spray method of distribution of the disinfectant mixture.

If the disinfectant mixture is mixed with air for 24 hours or more, complete cleaning and disinfection of the area is achieved.

Diffuser control can be: manual or remote, e.g. can be secured via Bluetooth.

The disinfectant mixture is used as a biocidal product to disinfect indoor air and surfaces.

The disinfectant mixture is continuously chemically deactivated from the surfaces by oxidation. The treated surfaces should not come into contact with food or animal feed for 60 minutes after application. It is recommended to rinse the surfaces with clean water and ventilate the area 60 minutes after applying the disinfectant mixture.

The diffuser with disinfectant mixture should only be used in a well-ventilated area.

The procedure of such disinfection is very simple and suitable for any type of operation. The products are not harmful to health and therefore it is possible for the device to work even when there are people in operation. The volume of the disinfectant mixture lasts in the diffuser on average two to six months, depending on the type of diffuser and the selected intensity.

The technology of disinfecting air using diffusers using a disinfectant mixture based on biocidal oils is not visible to the naked eye and therefore a number of tests in accredited laboratories had to be performed to demonstrate biocidal efficiency.

In addition, field tests were carried out: either by the gradient method, which compared the values of pathogens on petri dishes, or by the aroscopy method, where the aeroscope sucked microorganisms from the rooms, which it then evaluated from the internal medium. The measurement results showed a reduced amount of pathogens by up to 40%. Repeated testing confirmed these values.

The results of tests in accredited laboratories are shown in Table 2:

Table 2

The strain used Amount of CTU / ml / Contact time Incubation TS 1 min. 60 min. Concentration m% (V / V) Undiluted Staphylococcus aureus CCM 4516 N 3 x 10 ⁸ 3 x 10 ⁸ 24 h / 36 + - 1 ° C N No 3 x 10 ⁷ 3 x 10 ⁷ On the <10 <10 Log R 7 7

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The strain used Amount of CTU / ml / Contact time Incubation TS 1 min. 60 min. Concentration m% (V / V) Undiluted The strain used Quantity KTJ / ml / Contact time Incubation Incubation TS 1 min. 60 min. Concentration m% (V / V) Undiluted Pseudomonas aeruginosa CCM1961 N No Na Log R 2.1 x 10 ⁸ 2.1 x 10 ⁷ <10 7 2.1 x 10 ⁸ 2.1 x 10 ⁷ <10 7 24 h / 36 + - 1 ° C N Escherichia coli CCM4533 N No Na Log R 2.7 x 10 ⁸ 2.7 x 10 ⁷ <10 7 2.7 x 10 ⁸ 2.7 x 10 ⁷ <10 7 24 h / 36 + - 1 ° C N Enterococcus hirae CMM 4533 N No Na Log R 1.9 x 10 ⁸ 1.9 x 10 ⁷ <10 7 1.9 x 10 ⁸ 1.9 x 10 ⁷ <10 7 24 h / 36 + - 1 ° C N Enterococcus faecalis CCM 4224 N No Na Log R 2.3 x 10 ⁸ 2.3 x 10 ⁷ <10 7 2.3 x 10 ⁸ 2.3 x 10 ⁷ <10 7 24 h / 36 + - 1 ° C N Citrobacter koseri CCM 2535 N No Na Log R 3.7 x 10 ⁸ 3.7 x 10 ⁷ <10 7 3.7 x 10 ⁸ 3.7 x 10 ⁷ <10 7 24 h / 36 + - 1 ° C N Legionella pneumophila Subsp. Pneumophila CCM 7170 N No Na Log R 2.3 x 10 ⁸ 2.3 x 10 ⁷ <10 7 2.3 x 10 ⁸ 2.3 x 10 ⁷ <10 7 24 h / 36 + - ° C N Salmonella enterica Subsp. Enterica CCM 7189 N No Na Log R 4 x 10 ⁸ 4 x 10 ⁷ <10 7 4 x 10 ⁸ 4 x 10 ⁷ <10 7 24 h / 36 + - 1 ° C N Candida albicans CCM 8215 N No On the Log R 1.9 x 10 ⁷ 1.9 x 10 ⁶ <10 6 1.9 x 10 ⁷ 1.9 x 10 ⁶ <10 6 24 h / 36 + - 1 ° C N Aspergillus niger / brasiliensis CCM 8222 N No Na Log R 2.2 x 10 ⁷ 2.2 x 10 ⁶ <10 6 2.2 x 10 ⁷ 2.2 x 10 ⁶ <10 6 24 h / 36 + - 1 ° C N Clostridium difficile CCM 3593 N No On the Log R 2.1 x 10 ⁶ 2.1 x 10 ⁵ <10 5 2.1 x 10 ⁶ 2.1 x 10 ⁵ <10 5 24 h / 36 + - 1 ° C N Bacillus cereus CCM 2010 N No Na Log R 3.3 x 10 ⁶ 3.3 x 10 ⁵ <10 5 3.3 x 10 ⁶ 3.3 x 10 ⁵ <10 5 24 h / 36 + - 1 ° C N

Other results are as follows:

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Institute of Clinical Microbiology Hradec Králové

The protocol from the Institute of Clinical Microbiology in Hradec Králové tested the amount of pathogens in the air in the normal operation of the office using the gradient method. The total amount of bacteria decreased by 40% after 8 hours. As for molds, we have shown a static effect there, t. j. that the molds stopped spreading despite the humid and warm environment, they even decreased slightly. The test was carried out after only 8 hours of diffuser operation (a longer effect is achieved with long-term use of the diffuser).

The second protocol from the Institute of Clinical Microbiology in Hradec Králové tested the effect of the disinfectant mixture on the cultivation of two types of bacteria. Staphylococcus aureus disappeared almost completely after 8 hours. Escherichia coli has only partially disappeared, but this is due to its higher resistance (a greater effect is achieved with long-term use of the diffuser). The test was performed after 8 hours. The test results are shown graphically in FIG. 1 and FIG. 2.

Regional Office of Public Health Trnava

The test report from the Regional Public Health Office proved the effectiveness of the use of oil with a disinfectant element on several bacterial strains. The test result is shown in FIG. 3.

MEDIREX Central Laboratory, a. s., member of M EDI REX GROUP

In cooperation with the Specialized Hospital of St. Svorada Zobor, n. about. we have proven the disinfection efficiency of disinfection mixtures according to this technical solution applied to the room by means of New Aroma brand diffusers with a built-in atomizer.

Testing lasted for two days.

A special device, an aeroscope, sucked air and microorganisms out of the room, which it captured on an internal medium and later evaluated in the laboratory. After 24 hours of active operation of the diffusers with the disinfectant mixture, we repeated the aeroscopy. We compared the measured values, resulting in a decrease in pathogens by 60 to 100%.

Overview of figures in the drawings

FIG. 1 Graphical representation of the resulting number of Escherichia coli ATCC 25922 bacteria after exposure to air without a diffuser and after using a diffuser

FIG. 2 Graphical representation of the resulting number of Staphylococcus aureus ATCC 6358 after exposure to air without a diffuser and after using a diffuser

FIG. 3 Graphical representation of the results of testing a mixed population of bacteria in the air without a diffuser and after using a diffuser

FIG. 4 Basic scheme of the device - diffuser without safety lock

Examples of embodiments

Example 1

The disinfectant mixture contains active ingredients and other ingredients, the active ingredients being: N- (3-aminopropyl) -N-dodecylpropane-1,3-diamine at a concentration of 10,000 mg / kg 2-phenoxyethanol at a concentration of 5,000 mg / kg bis ( persulphate) bis (potassium) pentapotassium at a concentration of 5 000 mg / kg and the other ingredients are:

isopropyl myristate at a concentration of 902,000 mg / kg α-hexylcinnamaldehyde at a concentration of 10,000 mg / kg

4-tert-butylcyclohexyl acetate at 10,000 mg / kg eucalyptol at 10,000 mg / kg 1- (2,3,8,8-tetramethyl-1,2,3,4,5,6,7,8-octahydronaphthalene -2-yl) ethanone at a concentration of 10,000 mg / kg limonene at a concentration of 10,000 mg / kg linalol at a concentration of 10,000 mg / kg iodine resublimed at a concentration of 99.98% 3,000 mg / kg ethyl alcohol at a concentration of 10,000 mg / kg fragrance composition at a concentration of 5,000 mg / kg.

All components are mixed at room temperature.

SK 1-2022 U1

The air purification was carried out in a room with an area of up to 30 m ² and a diffuser 1 intended for home or office use was used.

The powerful aroma diffuser 1 flavored the space in the range of 30 m ² . It can be hung on a wall or placed freely on a flat surface. It is made of quality plastic, is quiet, the volume of the filling is 100 ml and lasts min. 30 days.

Diffuser 1 Mini works even without the need for power supply. The package includes two sets of rechargeable batteries. The battery lasts charged for at least 1 month, while the second set can be powered by an electric cable.

Diffuser 1 is suitable for all types of smaller spaces that need to be discreetly flavored without the need for an electrical outlet, such as Toilets, bathrooms, wardrobes, entrance halls, waiting rooms.

Mini diffuser 1 uses atomization technology to create micron particles of essential oil that fill the space and last longer than conventional air fresheners. It is also suitable for sanitary facilities.

Basic technical parameters of the diffuser:

Installation: wall mounting, free-standing diffuser-table

Control: internal computer panel - automatically adjustable

The method of disinfecting air and surfaces was carried out by first preparing a disinfectant mixture in an amount determined according to the size and parameters of vessel 2 of the diffuser 1. The disinfectant mixture was placed in diffuser 1 by opening the diffuser vessel 2, which was then filled with undiluted disinfectant mixture. and this was inserted into the head 4 of the diffuser 1. The diffuser 1 was then set to the determined power intensity and time according to the size of the disinfected space and the degree of its contamination. The diffuser 1 was switched on via the control 5 of the diffuser 1. Then the disinfectant mixture was mixed with air by automatic diffusion through the head 4 of the diffuser 1, and after the set time had elapsed 1 the diffuser 1 was automatically switched off.

Before and after the diffuser was connected, testing of the change in the quantity of microbiological air pollution was performed in order to quantify the antibacterial and antifungal effects of the disinfectant mixture used as a filler in the diffuser.

Diffuser setting 1: Interval 60 s, exposure time 8 hours, intensity level 4.

Aids: agar media to determine the total concentration of the mixed bacterial population (pCA agar lot 02136 exP. 2020-10-01, LN / S, SK) agar media to determine the total concentration of the mixed fungal population (YCG agar lot 0 -23, LMS, CZ ).

Day 1:

detection of air pollution in a room without diffuser 1 - procedure:

- during 8 hours placing two open agar plates (1x PCA, 1x YGC) on a table in the middle of the room, closing the agar plates after 4 hours;

- during 12 hours placement of two agar plates (1x PCA, 1x YGC), sealing of agar plates after 4 hours;

Incubation of plates in PCA thermostat at 37C, YGC at 22C for 3-5 days.

Day 2:

detection of air pollution in a room with a functioning diffuser - procedure:

- 8 o'clock the work of diffuser 1, which was placed on a table in the middle of the room;

- placing two open agar plates (1x PCA, 1x YGC) on a table in the middle of the room at a distance of 50 cm from diffuser 1, closing the agar plates after 4 hours;

- 12 o'clock placing two open agar plates ltx niA, 1x yGC, sealing the agar plates after 4 hours;

- termination of diffuser 1;

- incubation of the plates in a PCA thermostat at a temperature of 37 ° C. C, YGC at 22 ° C. C for 3-5 days;

- manual counting of the number of colonies on agar plates and preparation of a test report.

The results:

Table no. 3

The date Mixed population of bacteria in the air Time 8-12 hours 12-16 o'clock in sum % Without diffuser September 8, 2020 37 33 70 100% During diffuser operation 9. 9. 2020 26 17 43 61.4%

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Table no. 4

The date Mixed population of yeasts and fungi in the air Time 8-12 hours 12-16 o'clock in sum % Without diffuser September 8, 2020 57 46 103 100% During diffuser operation 9. 9. 2020 50 47 97 94.2%

During the operation of diffuser 1, the mixed population of bacteria in the air decreased by 38.6%, and the mixed population of yeasts and fungi in the air by 5.8%.

Example 2

The preparation of the disinfectant mixture and the air disinfection procedure were the same as in Example 1. The air purification was carried out on an area of up to 100 m ² , a 1 Basic diffuser intended for home or office use was used.

The 1 Basic diffuser is preferably designed for wall mounting, but it can also be placed anywhere in the room.

The diffuser 1 has a metal construction with a glass display, and is equipped with a power pump. The filling in a volume of 200 ml is sufficient for at least 60 days, while the change of the disinfectant mixture is very simple.

Diffuser 1 is used in all types of smaller spaces - such as small businesses, offices, apartments, waiting rooms, surgeries, locker rooms.

Basic technical parameters of the diffuser:

Installation: wall mounting, free-standing diffuser-table

Control: internal computer panel - automatically adjustable

Example 3

The preparation of the disinfectant mixture and the air disinfection procedure were the same as in Example 1.

The air purification took place in a room up to 100 m ² , and such a solution is especially suitable for commercial premises and operations.

A free-standing diffuser 1 was used, which was equipped with an intuitive odor intensity control covering an area of up to 100 m ² .

Diffuser 1 is made of aluminum and its filling volume is 100 ml.

Basic technical parameters of the diffuser:

Installation: free-standing diffuser - table

Control: intuitive touch screen

Test performed by Mandario Company s.r.o., Prague, CZ

Protocol:

Testing of the change in the quantity of the defined inoculum of selected bacteria in a room with a natural atmosphere and in a room with a diffuser connected.

Objective: to quantify the antibacterial and antifungal effects of air containing substances released from the diffuser against a test performed in a room with a normal atmosphere.

2 strains were selected for testing: Escherichia coli (indicator of faecal contamination), Staphylococcus aureus (indicator of hand and airway secretion),

Apparatus and cartridge used in the test: NEW AROMA diffuser, contents of the SCENT OIL Medical Gare with Disinfectant Element cartridge.

Time setting on the device: interval 30 sec., Timer 8 hrs., Power intensity level 4

Tools:

- Meuller-Hinton agar growth agar media (batch 4271055)

- reference strains of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6358, inoculum diluents

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Test area used:

Laboratories were used, in this case 2 rooms measuring 7.5 m x 4 m at rest, t. j. without activity and movement of persons.

The test in both rooms took place on the same day.

Work procedure:

- dilution of a defined inoculum of reference bacterial strains

- preparation of 8 agar plates for each microorganism

- placement of open agar plates in room no. 1 and to room no. 2 for 8 hours

- placing the diffuser in room 1 at a distance of 30 cm from the agar plates

- after 4 hours (A), 6 p.m. (B), 8 p.m. (C) 1 + 1 (one each with S. aureus and E. coli) agar plates were sealed and transferred to a thermostat

- after 8 o'clock diffuser 1 has been terminated

- incubation of the plates in a thermostat at 37 ° C was then started. C

- after 24 hours the last pair of dishes (D) was closed and taken to the thermostat

- counting of the number of colonies on plates (A), (B), (C) was performed after 24 and after 48 hours - counting of the number of colonies on plates (D) was performed after 24 and after 48 hours.

The results:

1. Testing of the quantity change of the defined Escherichia coli ATCC 25922 inoculum in a room with a natural atmosphere and in a room with a diffuser connected.

Table no. 5

Escherichia coli bacterium ATCC 25922

9. 9. 2020 Escherichia coli ATCC 25922 Without diffuser During diffuser operation Number of CFU Number of CFU Change % After 4 hours 239 251 +5.0 After 6 hours 231 262 +13.4 After 8 o'clock 315 270 -14.3 After 24 hours 248 211 -14.9

During the operation of the NEW ARÓMA diffuser 1, the quantity of the defined Escherichia coli ATCC 25922 inoculum decreased by 14.3% after the measurement after 8 hours, and decreased by 14.9% after the measurement after 24 hours compared to the control measurement.

2. Testing of the change in the quantity of the defined inoculum Staphylococcus aureus ATGC 6358 in the room with the natural atmosphere and in the room with the connected diffuser of the aromatic substance with disinfection of the components

Table no. 6

ATCC 6358 aureus

Sep 17, 2020 Staphylococcus aureus ATCC 6358 Without diffuser During diffuser operation Number of KTJ Number of KTJ Change % After 4 hours 1 276 1061 -16.8 After 6 hours 934 503 -46.1 After 8 o'clock 914 128 -86.0 After 24 hours 854 18 -97.9

During the operation of the NEW ARÓMA diffuser 1, the quantity of the defined Escherichia coli ATCC 25922 inoculum decreased by 46.1% after 6 hours, and by 86% after 8 hours, and 97.9% decreased after 24 hours compared to the control measurement.

Example 4

The preparation of the disinfectant mixture and the air disinfection procedure were the same as in Example 1.

A professional diffuser 1 was used for connection to air conditioning, designed for large areas from 1000 m ³ to 15,000 m ³ , which use the HVAC system (air conditioning).

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This type of diffuser 1 is equipped with a powerful pump which has a service life of min. 8,000 hours. It can be supplied with a 500 ml or 5 liter container. It is designed for large spaces such as hotel halls, dance halls, shopping malls, production halls.

Basic technical parameters of the diffuser:

Installation: connection to HVAC

Control: external computer panel - automatically adjustable

Example 5

The preparation of the disinfectant mixture and the air disinfection procedure were the same as in Example 1.

A small portable diffuser 1 was used, which is suitable for a car, apartment or office. Ideally covers 27 m ³ of space. The 20 ml cartridge lasts up to 100 hours. It has a selectable 3-degree intensity.

It is made of aluminum and the volume of the filling - disinfectant mixture is 20 ml.

Basic technical parameters of diffuser 1:

Installation: free-standing 1-table diffuser

Control: intuitive touch screen

Example 6

The preparation of the disinfectant mixture and the air disinfection procedure were the same as in Example 1. A diffuser 1 was used for connection to air conditioning and recuperation, which belongs to the group of professional technical equipment. Thanks to the connection to air conditioning, the device can more efficiently and evenly disperse the aroma throughout the space, up to 1,000 m ³ while maintaining a minimum consumption of essential oil.

The device is suitable for all types of smaller spaces equipped with air conditioning - such as small businesses, offices, apartments, waiting rooms, surgeries, changing rooms.

Basic technical parameters of diffuser 1:

Installation: connection to HVAC

Control: external computer panel.

Example 7

The preparation of the disinfectant mixture and the air disinfection procedure were the same as in Example 1.

A professional diffuser 1 was used for connection to air conditioning, designed for large spaces up to 15,000 m ³ , which use the HVAC system (air conditioning).

The device has two independent computer-controlled control panels, so it is possible to independently flavor two spaces or one space with two types of scent. This is especially important in areas with a changing purpose of use (eg an energizing fresh aroma is used during the day, a soft soothing scent in the evening, or in production halls with night operation, where, on the contrary, an energizing scent is needed at night).

This type of device is equipped with a powerful pump, which has a service life of min. 8,000 hours. It can be used with a 500 ml or 5 liter container. It is designed for large spaces such as hotel halls, dance halls, shopping malls, production halls.

Basic technical parameters of the diffuser:

Installation: connection to HVAC

Control: double external computer panel - automatically adjustable

Example 8

The preparation of the disinfectant mixture and the air disinfection procedure were the same as in Example 1.

It was about air purification in the premises of commercial premises.

A powerful diffuser 1 with a fan and a touch screen was used. The area covered in this case was up to 300 m ² . The 500 ml cartridge lasts up to 90 days.

Diffuser 1 is also suitable for dispensaries, waiting rooms, retail outlets and smaller hotel lobbies and for school facilities.

Basic technical parameters of the diffuser:

Installation: free-standing diffuser - table, wall mounting is also possible

SK 1-2022 U1

Control: intuitive touch screen.

Industrial applicability

The disinfectant mixture and the disinfection method according to the present solution are used for disinfecting air and interior surfaces of various kinds, e.g. spaces in homes, offices, but also large spaces such as hotel halls, dance halls, shopping malls, production halls, hospitals.

Claims (8)

SK 1-2022 U1 PROTECTION REQUIREMENTS Disinfectant composition, characterized in that it contains active ingredients and other ingredients, the active ingredients being: N- (3-aminopropyl) -N-dodecylpropane-1,3-cliamine at a concentration of 10,000 mg / kg; 2-phenoxyethanol at a concentration of 5,000 mg / kg; bis (persulphate) -bis (sulphate) pentapotassium at a concentration of 5 000 mg / kg and the other ingredients are: isopropyl myristate at a concentration of 902 000 mg / kg; α-hexylcinnamaldehyde at a concentration of 10,000 mg / kg; 4-tert-butylcyclohexyl acetate at a concentration of 10,000 mg / kg; eucalyptol at a concentration of 10,000 mg / kg; 1- (2,3,8,8-tetramethyl-1,2,3,4,5,6,7,8-octahydro-naphthalen-2-yl) ethanone at a concentration of 10,000 mg / kg; limonene at a concentration of 10,000 mg / kg; linalol at a concentration of 10,000 mg / kg; iodine resublimed at a concentration of 99.98% 3,000 mg / kg; ethyl alcohol at a concentration of 10,000 mg / kg; fragrance composition at a concentration of 5,000 mg / kg. A method of disinfecting air and surfaces in enclosed spaces using a disinfectant composition according to claim 1, characterized in that the disinfection composition is prepared by mixing all components at room temperature in an amount determined by the size and parameters of the diffuser vessel (2), then the diffuser container (2) is opened and, if necessary, the safety lock of the cap is removed, then the diffuser container (2) (1) is filled with undiluted disinfectant mixture and inserted into the diffuser head (4) (1), then the diffuser (1) is set to the specified power intensity and exposure time, which are set according to the size of the disinfected space and the degree of its contamination, the exposure time is at least 1 hour, and the diffuser (1) is switched on via the diffuser (1) control (5), then the disinfectant mixture is mixed with air by automatic diffusion through the head (4) of the diffuser (1), and after the set time has elapsed, the diffuser (1) automatically shuts off, and if the diffuser (1) supports such a function, has been deactivated. Method for disinfecting air and surfaces in enclosed spaces according to claim 2, characterized in that the diffuser (1) operates on the basis of cold diffusion technology, which uses a spray method for distributing the disinfectant mixture. A method of disinfecting air and surfaces in enclosed spaces according to claim 2, characterized in that the disinfectant mixture is mixed with air for 24 hours or more. Method for disinfecting air and surfaces in enclosed spaces according to claim 2, characterized in that the control (5) of the diffuser (1) is manual. Method for disinfecting air and surfaces in enclosed spaces according to claim 2, characterized in that the control (5) of the diffuser (1) is remote. A method of disinfecting air and surfaces in enclosed spaces according to claim 6, characterized in that the control (5) of the diffuser (1) is provided via Bluetooth. Use of a disinfectant composition according to claim 1 as a biocidal product for disinfecting indoor air and surfaces. 2 drawings