SI3218514T1 - Napovedovanje proizvodnje genetsko stabilnih rekombinantnih proteinov v zgodnjem razvoju celične linije - Google Patents

Napovedovanje proizvodnje genetsko stabilnih rekombinantnih proteinov v zgodnjem razvoju celične linije

Info

Publication number
SI3218514T1
SI3218514T1 SI201530923T SI201530923T SI3218514T1 SI 3218514 T1 SI3218514 T1 SI 3218514T1 SI 201530923 T SI201530923 T SI 201530923T SI 201530923 T SI201530923 T SI 201530923T SI 3218514 T1 SI3218514 T1 SI 3218514T1
Authority
SI
Slovenia
Prior art keywords
predicting
cell line
recombinant protein
protein production
genetically stable
Prior art date
Application number
SI201530923T
Other languages
English (en)
Inventor
Uros Jamnikar
Kristina Gruden
Original Assignee
Lek Pharmaceuticals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lek Pharmaceuticals filed Critical Lek Pharmaceuticals
Publication of SI3218514T1 publication Critical patent/SI3218514T1/sl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/71—Assays involving receptors, cell surface antigens or cell surface determinants for growth factors; for growth regulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/906—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
    • G01N2333/9065—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on CH-NH groups of donors (1.5)
    • G01N2333/90655—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1) in general
    • G01N2333/90661—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1) in general with a definite EC number (1.5.1.-)
    • G01N2333/90666—Dihydrofolate reductase [DHFR] (1.5.1.3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SI201530923T 2014-11-12 2015-11-12 Napovedovanje proizvodnje genetsko stabilnih rekombinantnih proteinov v zgodnjem razvoju celične linije SI3218514T1 (sl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14192809 2014-11-12
EP15797902.2A EP3218514B1 (en) 2014-11-12 2015-11-12 Predicting genetically stable recombinant protein production in early cell line development
PCT/EP2015/076390 WO2016075216A1 (en) 2014-11-12 2015-11-12 Predicting genetically stable recombinant protein production in early cell line development

Publications (1)

Publication Number Publication Date
SI3218514T1 true SI3218514T1 (sl) 2019-11-29

Family

ID=51947148

Family Applications (1)

Application Number Title Priority Date Filing Date
SI201530923T SI3218514T1 (sl) 2014-11-12 2015-11-12 Napovedovanje proizvodnje genetsko stabilnih rekombinantnih proteinov v zgodnjem razvoju celične linije

Country Status (6)

Country Link
US (1) US10913984B2 (sl)
EP (1) EP3218514B1 (sl)
DK (1) DK3218514T3 (sl)
PL (1) PL3218514T3 (sl)
SI (1) SI3218514T1 (sl)
WO (1) WO2016075216A1 (sl)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024048079A1 (ja) * 2022-08-31 2024-03-07 富士フイルム株式会社 有用物質を産生するクローンの産生安定性を予測する方法、情報処理装置、プログラムおよび予測モデル生成方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136536A (en) 1997-10-29 2000-10-24 Genetics Institute, Inc. Rapid generation of stable mammalian cell lines producing high levels of recombinant proteins
CA2574953A1 (en) 2004-07-26 2006-02-09 Dow Global Technolgies Inc. Process for improved protein expression by strain engineering
US20080070268A1 (en) 2006-04-21 2008-03-20 Wyeth Differential expression profiling analysis of cell culture phenotypes and the uses thereof
US20110200987A1 (en) 2006-08-24 2011-08-18 Government Of The United States Of America, As Represented By The Secretary, Department Of Health Compositions and methods for modifying cellular properties
US20090017460A1 (en) 2007-06-15 2009-01-15 Wyeth Differential expression profiling analysis of cell culture phenotypes and uses thereof
GB0813253D0 (en) 2008-07-18 2008-08-27 Univ Aston Protein expression system
JP2012526535A (ja) 2009-05-15 2012-11-01 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング ＮｏＣＲの発現が低下した改良細胞系及びその使用
EP2453007A1 (en) * 2010-11-10 2012-05-16 LEK Pharmaceuticals d.d. Nuclear DNA content of CHO cell lines as a selection criterion in screening of the best recombinant protein producers
WO2012102793A2 (en) * 2010-12-10 2012-08-02 Zirus, Inc. Mammalian genes involved in toxicity and infection

Also Published As

Publication number Publication date
US20170314082A1 (en) 2017-11-02
PL3218514T3 (pl) 2020-01-31
US10913984B2 (en) 2021-02-09
EP3218514B1 (en) 2019-07-24
DK3218514T3 (da) 2019-10-21
EP3218514A1 (en) 2017-09-20
WO2016075216A1 (en) 2016-05-19

Similar Documents

Publication Publication Date Title
IL261979A (en) A mammalian cell line for protein production and a breeding library
IL246080B (en) Saliva bio-sensors and biofuel cells
SG11201803493UA (en) Genetically modified cells and uses thereof
SG10202005876VA (en) Cell reprogramming
PL3134724T3 (pl) Komórka elektrochemiczna
HK1248272A1 (zh) 宿主細胞蛋白質修飾
IL247491A0 (en) Expression constructs and methods for selecting host cells expressing polypeptides
DK3569697T3 (da) Stamcelleterapi i endometriumpatologier
HUE054259T2 (hu) Sejttenyészet
SG11201702964UA (en) Methods of recombinant protein expression in a cell comprising reduced udp-galactose transporter activity
IL252570A0 (en) in the colon of a small cell
SI3218514T1 (sl) Napovedovanje proizvodnje genetsko stabilnih rekombinantnih proteinov v zgodnjem razvoju celične linije
GB201817435D0 (en) Detecting cell vitality
PL3218478T3 (pl) Przewidywanie produktywności we wczesnym rozwoju linii komórkowej
GB2539233B (en) Electrochemical cell
EP2970916A4 (en) LEPIDOPTER INSECT CELLS SUITABLE FOR THE PRODUCTION OF RECOMBINANT PROTEINS
GB201521571D0 (en) Electrochemical cell
GB201510142D0 (en) Electrochemical cells
HUE046774T2 (hu) Telepcella
GB201506317D0 (en) Hepatocyte-like cells and related cell lines
GB201421342D0 (en) Cell culture
GB201420709D0 (en) Electrochemical cells and components thereof