SG48047A1 - Polypeptides possessing a nitrilase activity dna sequence coding for said polypeptides expression cassettes and host microorganisms enabling them to be obtained (see file for full - Google Patents

Polypeptides possessing a nitrilase activity dna sequence coding for said polypeptides expression cassettes and host microorganisms enabling them to be obtained (see file for full

Info

Publication number
SG48047A1
SG48047A1 SG1996006647A SG1996006647A SG48047A1 SG 48047 A1 SG48047 A1 SG 48047A1 SG 1996006647 A SG1996006647 A SG 1996006647A SG 1996006647 A SG1996006647 A SG 1996006647A SG 48047 A1 SG48047 A1 SG 48047A1
Authority
SG
Singapore
Prior art keywords
polypeptides
full
dna sequence
expression cassettes
sequence coding
Prior art date
Application number
SG1996006647A
Inventor
Edith Cerbelaud
Joel Crouzet
Sophie Levy-Schill
Dominique Petre
Original Assignee
Rhone Poulenc Chimie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhone Poulenc Chimie filed Critical Rhone Poulenc Chimie
Publication of SG48047A1 publication Critical patent/SG48047A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/05—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in nitriles (3.5.5)
    • C12Y305/05001—Nitrilase (3.5.5.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG1996006647A 1992-08-10 1993-08-06 Polypeptides possessing a nitrilase activity dna sequence coding for said polypeptides expression cassettes and host microorganisms enabling them to be obtained (see file for full SG48047A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9209882A FR2694571B1 (en) 1992-08-10 1992-08-10 Polypeptides having nitrilase activity, DNA sequence coding for said polypeptides, expression cassettes and host microorganisms allowing their production.

Publications (1)

Publication Number Publication Date
SG48047A1 true SG48047A1 (en) 1998-04-17

Family

ID=9432757

Family Applications (1)

Application Number Title Priority Date Filing Date
SG1996006647A SG48047A1 (en) 1992-08-10 1993-08-06 Polypeptides possessing a nitrilase activity dna sequence coding for said polypeptides expression cassettes and host microorganisms enabling them to be obtained (see file for full

Country Status (10)

Country Link
EP (1) EP0596812B1 (en)
JP (1) JPH0751070A (en)
KR (1) KR940004057A (en)
BR (1) BR9305280A (en)
CA (1) CA2103616A1 (en)
DE (1) DE69314280T2 (en)
ES (1) ES2108850T3 (en)
FR (1) FR2694571B1 (en)
MX (1) MX9304825A (en)
SG (1) SG48047A1 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU669951B2 (en) * 1994-01-12 1996-06-27 Rhone-Poulenc Nutrition Animale Polypeptides possessing a nitrilase activity, DNA sequence coding for said polypeptides, expression cassettes and host microorganisms enabling them to be obtained, and method of converting nitriles to carboxylates by means of said polypeptides
US5858736A (en) * 1996-05-17 1999-01-12 E. I. Du Pont De Nemours And Company Preparation of lactams from aliphatic α,ω-dinitriles
FR2780416B1 (en) * 1998-06-10 2002-12-20 Rhone Poulenc Nutrition Animal INDUSTRIAL PROCESS FOR PRODUCING HETEROLOGOUS PROTEINS IN E. COLI AND STRAINS USEFUL FOR THE PROCESS
US6365376B1 (en) 1999-02-19 2002-04-02 E. I. Du Pont De Nemours And Company Genes and enzymes for the production of adipic acid intermediates
US6551804B2 (en) 1999-07-12 2003-04-22 E. I. Du Pont De Nemours And Company Process for preparing 4-cyanopentanoic acid
US7300775B2 (en) 1999-12-29 2007-11-27 Verenium Corporation Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents
US7521216B2 (en) 1999-12-29 2009-04-21 Verenium Corporation Nitrilases and methods for making and using them
US7608445B1 (en) 1999-12-29 2009-10-27 Verenium Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
JP4755796B2 (en) 2000-03-31 2011-08-24 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー Isolation and expression of the gene for nitrilase from Acidovoraxfacilis 72W
US6562603B2 (en) 2000-08-04 2003-05-13 E. I. Du Pont De Nemours And Company 3-hydroxycarboxylic acid production and use in branched polymers
EP1320594A2 (en) 2000-09-30 2003-06-25 E.I. Du Pont De Nemours And Company Exbb-exbd-tonb gene cluster of pseudomonas putida dot-t1e conferring tolerance to aromatic compounds and resistance to antibiotics
FR2822460B1 (en) * 2001-03-26 2005-04-29 Rhodia Chimie Sa PROCESS FOR THE ENANTIOSELECTIVE PREPARATION OF OPTICALLY ACTIVE CARBOXYLIC ACIDS BY ENZYMATIC HYDROLYSIS OF NITRILES
US6670158B2 (en) * 2002-02-05 2003-12-30 E. I. Du Pont De Nemours And Company Method for producing methacrylic acid acrylic acid with a combination of enzyme catalysts
US7198926B2 (en) 2003-05-08 2007-04-03 E. I. Du Pont De Nemours And Company Preparation of (E)- and (Z)-2-methyl-2-butenoic acids
US7148051B2 (en) 2004-08-16 2006-12-12 E. I. Du Pont De Nemours And Company Production of 3-hydroxycarboxylic acid using nitrilase
DE102004043748A1 (en) * 2004-09-10 2006-03-16 Degussa Ag Biocatalyst for the hydrolysis of cyanide
KR101312262B1 (en) * 2006-02-23 2013-09-25 삼성에스디아이 주식회사 Polymer membrane, a method for preparing the polymer membrane and a fuel cell employing the same
US9217164B2 (en) 2007-03-01 2015-12-22 Basf Enzymes Llc Nitrilases, nucleic acids encoding them and methods for making and using them
KR101223664B1 (en) * 2011-01-26 2013-01-17 재단법인 경북해양바이오산업연구원 Method for preparing a carboxylic acid using nitrilase RMN1
GB2554708A (en) * 2016-10-05 2018-04-11 Chemoxy Int Process for converting nitriles
MX2021008768A (en) * 2019-01-22 2022-01-06 Basf Se Method for production of 4-cyano benzoic acid or salts thereof.
CN112359036B (en) * 2020-11-30 2022-03-08 浙江工业大学 Nitrilase mutant with improved catalytic activity and reaction specificity and application thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4629700A (en) * 1983-11-30 1986-12-16 Standard Oil Company (Indiana) Selective conversion of cyano compounds to amides and carboxylic acids
US4810648A (en) * 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
DE3926103A1 (en) * 1989-08-08 1991-02-14 Hoechst Ag METHOD FOR THE BIOCATALYTIC CORRECT CHAIN FOLDING OF DENATURED RECOMBINANT FUSION PROTEINS

Also Published As

Publication number Publication date
EP0596812A1 (en) 1994-05-11
KR940004057A (en) 1994-03-14
CA2103616A1 (en) 1994-02-11
ES2108850T3 (en) 1998-01-01
MX9304825A (en) 1994-05-31
DE69314280D1 (en) 1997-11-06
BR9305280A (en) 1994-06-28
EP0596812B1 (en) 1997-10-01
FR2694571B1 (en) 1994-11-04
DE69314280T2 (en) 1998-02-05
FR2694571A1 (en) 1994-02-11
JPH0751070A (en) 1995-02-28

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