SG11202111649PA - Nucleic acid constructs and methods for their manufacture - Google Patents
Nucleic acid constructs and methods for their manufactureInfo
- Publication number
- SG11202111649PA SG11202111649PA SG11202111649PA SG11202111649PA SG11202111649PA SG 11202111649P A SG11202111649P A SG 11202111649PA SG 11202111649P A SG11202111649P A SG 11202111649PA SG 11202111649P A SG11202111649P A SG 11202111649PA SG 11202111649P A SG11202111649P A SG 11202111649PA
- Authority
- SG
- Singapore
- Prior art keywords
- manufacture
- methods
- nucleic acid
- acid constructs
- constructs
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
- C12N2310/151—Nucleic acids forming more than 2 strands, e.g. TFOs more than 3 strands, e.g. tetrads, H-DNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/52—Physical structure branched
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/125—Rolling circle
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1905651.4A GB201905651D0 (en) | 2019-04-24 | 2019-04-24 | Nucleic acid constructs and methods for their manufacture |
| PCT/GB2020/051003 WO2020217057A1 (en) | 2019-04-23 | 2020-04-23 | Nucleic acid constructs and methods for their manufacture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202111649PA true SG11202111649PA (en) | 2021-11-29 |
Family
ID=66641152
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202111649PA SG11202111649PA (en) | 2019-04-24 | 2020-04-23 | Nucleic acid constructs and methods for their manufacture |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20220195415A1 (en) |
| EP (1) | EP3959336A1 (en) |
| JP (1) | JP2022530432A (en) |
| KR (1) | KR20220003571A (en) |
| CN (1) | CN113874504A (en) |
| AU (1) | AU2020262371A1 (en) |
| BR (1) | BR112021021333A2 (en) |
| CA (1) | CA3137840A1 (en) |
| GB (1) | GB201905651D0 (en) |
| IL (1) | IL287468A (en) |
| MX (1) | MX2021013011A (en) |
| SG (1) | SG11202111649PA (en) |
| WO (1) | WO2020217057A1 (en) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| KR20220104847A (en) | 2017-06-30 | 2022-07-26 | 인스크립타 인코포레이티드 | Automated cell processing methods, modules, instruments, and systems |
| US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| CN113939593A (en) | 2019-06-06 | 2022-01-14 | 因思科瑞普特公司 | Treatment for recursive nucleic acid-guided cell editing |
| WO2021102059A1 (en) | 2019-11-19 | 2021-05-27 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
| AU2020407048A1 (en) | 2019-12-18 | 2022-06-09 | Inscripta, Inc. | Cascade/dCas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| US11299731B1 (en) | 2020-09-15 | 2022-04-12 | Inscripta, Inc. | CRISPR editing to embed nucleic acid landing pads into genomes of live cells |
| GB202014751D0 (en) * | 2020-09-18 | 2020-11-04 | Lightbio Ltd | Targeting vector |
| WO2022125329A1 (en) * | 2020-12-07 | 2022-06-16 | Inscripta, Inc. | gRNA STABILIZATION IN NUCLEIC ACID-GUIDED NICKASE EDITING |
| CA3204158A1 (en) | 2021-01-04 | 2022-07-07 | Juhan Kim | Mad nucleases |
| US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5624803A (en) * | 1993-10-14 | 1997-04-29 | The Regents Of The University Of California | In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom |
| US20050118616A1 (en) * | 2002-08-16 | 2005-06-02 | Kawashima Tadashi R. | Amplification of target nucleotide sequence without polymerase chain reaction |
| US20050153333A1 (en) * | 2003-12-02 | 2005-07-14 | Sooknanan Roy R. | Selective terminal tagging of nucleic acids |
| ATE483032T1 (en) * | 2005-04-12 | 2010-10-15 | Olink Ab | METHOD FOR PRODUCING OLIGONUCLEOTIDES |
| GB201321123D0 (en) * | 2013-11-29 | 2014-01-15 | Linea Ab Q | Amplification of circular molecules |
| JP6712948B2 (en) * | 2013-12-12 | 2020-06-24 | ザ・ブロード・インスティテュート・インコーポレイテッド | Compositions and methods of using the CRISPR-cas system in nucleotide repeat disorders |
| SI3354732T1 (en) * | 2014-06-23 | 2020-07-31 | Regeneron Pharmaceuticals, Inc. | Nuclease-mediated dna assembly |
| GB201413718D0 (en) * | 2014-08-01 | 2014-09-17 | Olink Ab | Method for selecting a target nucleic acid sequence |
| CA2975855A1 (en) * | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Compositions and methods for synthetic gene assembly |
| SG11201809002RA (en) * | 2016-04-29 | 2018-11-29 | Univ Nanyang Tech | G-quadruplex-containing antisense oligonucleotides |
| EP3763823A1 (en) | 2016-08-16 | 2021-01-13 | Touchlight IP Limited | Closed linear dna production |
| EP3678710A4 (en) | 2017-09-08 | 2021-06-09 | Generation Bio Co. | Modified closed-ended dna (cedna) |
| EP3692154A1 (en) * | 2017-10-02 | 2020-08-12 | Genedit Inc. | Modified cpf1 guide rna |
| MA51626A (en) | 2018-01-19 | 2020-11-25 | Generation Bio Co | CLOSED END DNA VECTORS AVAILABLE FROM ACELLULAR SYNTHESIS AND PROCESS FOR OBTAINING DNA VECTORS |
-
2019
- 2019-04-24 GB GBGB1905651.4A patent/GB201905651D0/en not_active Ceased
-
2020
- 2020-04-23 CA CA3137840A patent/CA3137840A1/en active Pending
- 2020-04-23 CN CN202080038141.6A patent/CN113874504A/en active Pending
- 2020-04-23 EP EP20722375.1A patent/EP3959336A1/en active Pending
- 2020-04-23 AU AU2020262371A patent/AU2020262371A1/en active Pending
- 2020-04-23 WO PCT/GB2020/051003 patent/WO2020217057A1/en active Application Filing
- 2020-04-23 US US17/605,474 patent/US20220195415A1/en active Pending
- 2020-04-23 JP JP2021563132A patent/JP2022530432A/en active Pending
- 2020-04-23 SG SG11202111649PA patent/SG11202111649PA/en unknown
- 2020-04-23 KR KR1020217038099A patent/KR20220003571A/en active Search and Examination
- 2020-04-23 BR BR112021021333A patent/BR112021021333A2/en unknown
- 2020-04-23 MX MX2021013011A patent/MX2021013011A/en unknown
-
2021
- 2021-10-21 IL IL287468A patent/IL287468A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN113874504A (en) | 2021-12-31 |
| KR20220003571A (en) | 2022-01-10 |
| BR112021021333A2 (en) | 2022-01-18 |
| AU2020262371A1 (en) | 2021-11-18 |
| JP2022530432A (en) | 2022-06-29 |
| IL287468A (en) | 2021-12-01 |
| WO2020217057A1 (en) | 2020-10-29 |
| MX2021013011A (en) | 2022-01-24 |
| CA3137840A1 (en) | 2020-10-29 |
| GB201905651D0 (en) | 2019-06-05 |
| US20220195415A1 (en) | 2022-06-23 |
| EP3959336A1 (en) | 2022-03-02 |
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