SG11202105914QA - Specific electroporation and lysis of eukaryotic cells - Google Patents

Specific electroporation and lysis of eukaryotic cells

Info

Publication number
SG11202105914QA
SG11202105914QA SG11202105914QA SG11202105914QA SG11202105914QA SG 11202105914Q A SG11202105914Q A SG 11202105914QA SG 11202105914Q A SG11202105914Q A SG 11202105914QA SG 11202105914Q A SG11202105914Q A SG 11202105914QA SG 11202105914Q A SG11202105914Q A SG 11202105914QA
Authority
SG
Singapore
Prior art keywords
lysis
eukaryotic cells
specific electroporation
electroporation
specific
Prior art date
Application number
SG11202105914QA
Other languages
English (en)
Inventor
Klemens Wassermann
Terje Wimberger
Johannes Peham
Original Assignee
Ait Austrian Inst Tech Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ait Austrian Inst Tech Gmbh filed Critical Ait Austrian Inst Tech Gmbh
Publication of SG11202105914QA publication Critical patent/SG11202105914QA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Mechanical Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
SG11202105914QA 2018-12-12 2019-12-12 Specific electroporation and lysis of eukaryotic cells SG11202105914QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18211969.3A EP3666882A1 (fr) 2018-12-12 2018-12-12 Électroporation et lyse spécifiques de cellules eucaryotes
PCT/EP2019/084793 WO2020120651A1 (fr) 2018-12-12 2019-12-12 Électroporation et lyse spécifiques de cellules eucaryotes

Publications (1)

Publication Number Publication Date
SG11202105914QA true SG11202105914QA (en) 2021-07-29

Family

ID=64901311

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202105914QA SG11202105914QA (en) 2018-12-12 2019-12-12 Specific electroporation and lysis of eukaryotic cells

Country Status (8)

Country Link
US (1) US20220049239A1 (fr)
EP (2) EP3666882A1 (fr)
CN (1) CN113272416A (fr)
CA (1) CA3122890A1 (fr)
ES (1) ES2964636T3 (fr)
IL (1) IL283854A (fr)
SG (1) SG11202105914QA (fr)
WO (1) WO2020120651A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4105312A1 (fr) * 2021-06-17 2022-12-21 Bühler AG Procédé et système d'identification de conditions de traitement optimisées

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2025022A1 (fr) * 1990-03-07 1991-09-08 Dael R. Casnig Dispositif permettant l'electroporation sur place des cellules adherentes
US8226811B2 (en) 2005-11-02 2012-07-24 May-Ruben Technologies, Inc. High impedance system for generating electric fields and method of use
GB201006841D0 (en) * 2010-04-26 2010-06-09 Thomsen Lars Method, device and system for targetted cell lysis
WO2015044191A1 (fr) 2013-09-24 2015-04-02 Ait Austrian Institute Of Technology Gmbh Procédé pour isoler des agents pathogènes
SG11201900447SA (en) * 2016-07-21 2019-02-27 Maxcyte Inc Methods and compositions for modifying genomic dna

Also Published As

Publication number Publication date
CN113272416A (zh) 2021-08-17
EP3666882A1 (fr) 2020-06-17
ES2964636T3 (es) 2024-04-08
EP3894539A1 (fr) 2021-10-20
EP3894539B1 (fr) 2023-08-16
WO2020120651A1 (fr) 2020-06-18
CA3122890A1 (fr) 2020-06-18
US20220049239A1 (en) 2022-02-17
IL283854A (en) 2021-07-29
EP3894539C0 (fr) 2023-08-16

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