SG11202102817WA - Intron-based universal cloning methods and compositions - Google Patents

Intron-based universal cloning methods and compositions

Info

Publication number
SG11202102817WA
SG11202102817WA SG11202102817WA SG11202102817WA SG11202102817WA SG 11202102817W A SG11202102817W A SG 11202102817WA SG 11202102817W A SG11202102817W A SG 11202102817WA SG 11202102817W A SG11202102817W A SG 11202102817WA SG 11202102817W A SG11202102817W A SG 11202102817WA
Authority
SG
Singapore
Prior art keywords
intron
compositions
cloning methods
based universal
universal cloning
Prior art date
Application number
SG11202102817WA
Inventor
Wulf Leuschner
Werner Dittrich
Joerg Birkenfeld
Marion Schneider
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of SG11202102817WA publication Critical patent/SG11202102817WA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/313—Type II endonucleases, i.e. cutting outside recognition site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11202102817WA 2018-09-20 2019-09-19 Intron-based universal cloning methods and compositions SG11202102817WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18306220 2018-09-20
PCT/EP2019/075232 WO2020058438A1 (en) 2018-09-20 2019-09-19 Intron-based universal cloning methods and compositions

Publications (1)

Publication Number Publication Date
SG11202102817WA true SG11202102817WA (en) 2021-04-29

Family

ID=63832350

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202102817WA SG11202102817WA (en) 2018-09-20 2019-09-19 Intron-based universal cloning methods and compositions

Country Status (13)

Country Link
US (1) US11834693B2 (en)
EP (1) EP3853361A1 (en)
JP (1) JP2022501036A (en)
KR (1) KR20210060561A (en)
CN (1) CN113015801A (en)
AU (1) AU2019342866A1 (en)
BR (1) BR112021005115A2 (en)
CA (1) CA3113130A1 (en)
IL (1) IL281536A (en)
MX (1) MX2021003273A (en)
SG (1) SG11202102817WA (en)
TW (1) TW202026422A (en)
WO (1) WO2020058438A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL315140A (en) * 2022-03-08 2024-10-01 Amgen Inc Modular vector (modvec) system: a platform for construction of next generation expression vectors

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002303176A1 (en) * 2001-03-27 2002-10-15 University Of Delaware Genomics applications for modified oligonucleotides
DE10145553B4 (en) 2001-09-16 2006-06-08 Gene Architects Ag Nucleic acid for a cloning vector
WO2004015085A2 (en) * 2002-08-09 2004-02-19 California Institute Of Technology Method and compositions relating to 5’-chimeric ribonucleic acids
US20100291633A1 (en) 2007-09-03 2010-11-18 Thorsten Selmer Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
WO2010053820A1 (en) * 2008-10-29 2010-05-14 Trustees Of Boston University Sequence preserved dna conversion
GB0909660D0 (en) * 2009-06-04 2009-07-22 Gene Bridges Gmbh Method of altering nucleic acids
PL2816112T3 (en) * 2009-12-10 2019-03-29 Regents Of The University Of Minnesota Tal effector-mediated DNA modification
US8771985B2 (en) * 2010-04-26 2014-07-08 Sangamo Biosciences, Inc. Genome editing of a Rosa locus using zinc-finger nucleases
EP2395087A1 (en) 2010-06-11 2011-12-14 Icon Genetics GmbH System and method of modular cloning
BR112013025567B1 (en) * 2011-04-27 2021-09-21 Amyris, Inc METHODS FOR GENOMIC MODIFICATION
CA2854249C (en) * 2011-12-22 2022-05-03 F. Hoffmann-La Roche Ag Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides
CN104372414B (en) * 2012-10-25 2016-05-04 盛司潼 A kind of method that builds sequencing library
WO2014102104A1 (en) * 2012-12-31 2014-07-03 Boehringer Ingelheim International Gmbh Artificial introns
US10253321B2 (en) * 2013-05-01 2019-04-09 Dna2.0, Inc. Methods, compositions and kits for a one-step DNA cloning system
EP3036334A1 (en) * 2013-08-22 2016-06-29 E. I. du Pont de Nemours and Company A soybean u6 polymerase iii promoter and methods of use
EP3757116A1 (en) * 2013-12-09 2020-12-30 Sangamo Therapeutics, Inc. Methods and compositions for genome engineering
CN106148322A (en) * 2015-04-22 2016-11-23 王金 A kind of method of synthetic DNA
US10041079B2 (en) * 2015-11-30 2018-08-07 Synthetic Genomics, Inc. Compositions and methods for expressing genes in algae
WO2018148256A1 (en) * 2017-02-07 2018-08-16 The Regents Of The University Of California Gene therapy for haploinsufficiency
EP3401328A1 (en) 2017-05-10 2018-11-14 Bayer Pharma Aktiengesellschaft One step antibody humanization by golden gate based germline framework region shuffling
CN116848244A (en) * 2020-12-15 2023-10-03 新英格兰生物实验室公司 Compositions and methods for improving polynucleotide in vitro assembly
CA3213502A1 (en) * 2021-04-21 2022-10-27 Nathaniel Stephen Wang Alphavirus vectors containing universal cloning adaptors

Also Published As

Publication number Publication date
CA3113130A1 (en) 2020-03-26
TW202026422A (en) 2020-07-16
AU2019342866A1 (en) 2021-05-20
JP2022501036A (en) 2022-01-06
CN113015801A (en) 2021-06-22
US11834693B2 (en) 2023-12-05
MX2021003273A (en) 2021-07-16
EP3853361A1 (en) 2021-07-28
IL281536A (en) 2021-05-31
WO2020058438A1 (en) 2020-03-26
US20200115728A1 (en) 2020-04-16
KR20210060561A (en) 2021-05-26
BR112021005115A2 (en) 2021-06-15

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