SG11202102008XA - Method and apparatus for high throughput high efficiency transfection of cells - Google Patents

Method and apparatus for high throughput high efficiency transfection of cells

Info

Publication number
SG11202102008XA
SG11202102008XA SG11202102008XA SG11202102008XA SG11202102008XA SG 11202102008X A SG11202102008X A SG 11202102008XA SG 11202102008X A SG11202102008X A SG 11202102008XA SG 11202102008X A SG11202102008X A SG 11202102008XA SG 11202102008X A SG11202102008X A SG 11202102008XA
Authority
SG
Singapore
Prior art keywords
cells
efficiency transfection
high efficiency
high throughput
transfection
Prior art date
Application number
SG11202102008XA
Inventor
Vishal Tandon
Charles A Lissandrello
Jenna L Balestrini
Jonathan R Coppeta
Patricia A Swierk
Original Assignee
Charles Stark Draper Laboratory Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Charles Stark Draper Laboratory Inc filed Critical Charles Stark Draper Laboratory Inc
Publication of SG11202102008XA publication Critical patent/SG11202102008XA/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/16Microfluidic devices; Capillary tubes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/046Function or devices integrated in the closure
    • B01L2300/047Additional chamber, reservoir
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087Multiple sequential chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0887Laminated structure
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0493Specific techniques used
    • B01L2400/0496Travelling waves, e.g. in combination with electrical or acoustic forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
SG11202102008XA 2018-08-31 2019-08-30 Method and apparatus for high throughput high efficiency transfection of cells SG11202102008XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862725382P 2018-08-31 2018-08-31
PCT/US2019/049210 WO2020047504A2 (en) 2018-08-31 2019-08-30 Method and apparatus for high throughput high efficiency transfection of cells

Publications (1)

Publication Number Publication Date
SG11202102008XA true SG11202102008XA (en) 2021-03-30

Family

ID=67982179

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202102008XA SG11202102008XA (en) 2018-08-31 2019-08-30 Method and apparatus for high throughput high efficiency transfection of cells

Country Status (10)

Country Link
US (2) US11859162B2 (en)
EP (1) EP3844261A2 (en)
JP (1) JP2021534793A (en)
KR (1) KR20210089640A (en)
CN (1) CN113166706A (en)
AU (1) AU2019331113A1 (en)
CA (1) CA3111720A1 (en)
IL (1) IL281138A (en)
SG (1) SG11202102008XA (en)
WO (1) WO2020047504A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108435265A (en) * 2017-01-25 2018-08-24 富士康(昆山)电脑接插件有限公司 Microfluidic cartridge and its stacking test suite
US11878300B2 (en) 2018-09-04 2024-01-23 The Charles Stark Draper Laboratory, Inc. Method and device for high field strength electrotransfection of microvescicles and cells
EP4085982A1 (en) 2021-05-06 2022-11-09 ETH Zurich Acoustofluidic device and method for trapping particles in a fluid
CN114196532A (en) * 2021-11-01 2022-03-18 上海盟德生物科技有限公司 Multi-channel cell electrotransformation instrument
WO2024148320A1 (en) * 2023-01-06 2024-07-11 University Of Kentucky Research Foundation 3d microfluidic transfection devices and method of transfecting target cells with a transfection agent

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0814855A4 (en) 1995-03-10 2002-07-17 Theramed Inc Flow electroporation chamber and method
US6773669B1 (en) 1995-03-10 2004-08-10 Maxcyte, Inc. Flow electroporation chamber and method
US6090617A (en) * 1996-12-05 2000-07-18 Entremed, Inc. Flow electroporation chamber with electrodes having a crystalline metal nitride coating
US7029916B2 (en) * 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
SE522801C2 (en) * 2001-03-09 2004-03-09 Erysave Ab Apparatus for separating suspended particles from an ultrasonic fluid and method for such separation
DK1456345T3 (en) * 2001-08-22 2016-09-26 Maxcyte Inc Apparatus and method for electroporation of biological samples
EP2156178B1 (en) 2007-04-02 2011-12-21 Acoustic Cytometry Systems, Inc. Methods for enhanced analysis of acoustic field focused cells and particles
US9816086B2 (en) 2010-07-06 2017-11-14 The Ohio State University Dose and location controlled drug/gene/particle delivery to individual cells by nanoelectroporation
DE102014200505A1 (en) * 2014-01-14 2015-07-16 Robert Bosch Gmbh Container and device for the digestion and / or homogenization of a fluid and method for digesting and / or homogenizing a fluid
JP6846345B2 (en) 2014-11-14 2021-03-24 マサチューセッツ インスティテュート オブ テクノロジー Destruction and field delivery of compounds and compositions to cells
WO2016187535A2 (en) * 2015-05-20 2016-11-24 Zephyrus Biosciences, Inc. System and methods for electrophoretic separation and analysis of analytes
KR20180042422A (en) 2015-09-04 2018-04-25 룻거스, 더 스테이트 유니버시티 오브 뉴저지 High-efficiency, feedback-controlled electroporation microdevices for efficient molecular delivery to single cells
US11225638B2 (en) * 2016-04-04 2022-01-18 CyteQuest, Inc. System, device and method for electroporation of cells
CN107988070A (en) 2017-06-13 2018-05-04 北京呈诺医学科技有限公司 A kind of few cells electricity turns micro-fluidic chip, electricity turns sorter and application
CN107699485B (en) * 2017-11-06 2020-08-11 东南大学 Microelectrode flow control chip and parameter-adjustable single-cell electroporation device
WO2020014538A1 (en) 2018-07-12 2020-01-16 Gpb Scientific, Llc Microfluidic methods for the preparation of cells

Also Published As

Publication number Publication date
CA3111720A1 (en) 2020-03-05
WO2020047504A3 (en) 2020-04-09
AU2019331113A1 (en) 2021-03-18
US11859162B2 (en) 2024-01-02
US20200071727A1 (en) 2020-03-05
CN113166706A (en) 2021-07-23
IL281138A (en) 2021-04-29
KR20210089640A (en) 2021-07-16
US20240084236A1 (en) 2024-03-14
WO2020047504A2 (en) 2020-03-05
JP2021534793A (en) 2021-12-16
EP3844261A2 (en) 2021-07-07

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