SG11202102008XA - Method and apparatus for high throughput high efficiency transfection of cells - Google Patents
Method and apparatus for high throughput high efficiency transfection of cellsInfo
- Publication number
- SG11202102008XA SG11202102008XA SG11202102008XA SG11202102008XA SG11202102008XA SG 11202102008X A SG11202102008X A SG 11202102008XA SG 11202102008X A SG11202102008X A SG 11202102008XA SG 11202102008X A SG11202102008X A SG 11202102008XA SG 11202102008X A SG11202102008X A SG 11202102008XA
- Authority
- SG
- Singapore
- Prior art keywords
- cells
- efficiency transfection
- high efficiency
- high throughput
- transfection
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
- B01L2300/047—Additional chamber, reservoir
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/087—Multiple sequential chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0493—Specific techniques used
- B01L2400/0496—Travelling waves, e.g. in combination with electrical or acoustic forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Physics & Mathematics (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Electromagnetism (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201862725382P | 2018-08-31 | 2018-08-31 | |
PCT/US2019/049210 WO2020047504A2 (en) | 2018-08-31 | 2019-08-30 | Method and apparatus for high throughput high efficiency transfection of cells |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11202102008XA true SG11202102008XA (en) | 2021-03-30 |
Family
ID=67982179
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11202102008XA SG11202102008XA (en) | 2018-08-31 | 2019-08-30 | Method and apparatus for high throughput high efficiency transfection of cells |
Country Status (10)
Country | Link |
---|---|
US (2) | US11859162B2 (en) |
EP (1) | EP3844261A2 (en) |
JP (1) | JP2021534793A (en) |
KR (1) | KR20210089640A (en) |
CN (1) | CN113166706A (en) |
AU (1) | AU2019331113A1 (en) |
CA (1) | CA3111720A1 (en) |
IL (1) | IL281138A (en) |
SG (1) | SG11202102008XA (en) |
WO (1) | WO2020047504A2 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108435265A (en) * | 2017-01-25 | 2018-08-24 | 富士康(昆山)电脑接插件有限公司 | Microfluidic cartridge and its stacking test suite |
US11878300B2 (en) | 2018-09-04 | 2024-01-23 | The Charles Stark Draper Laboratory, Inc. | Method and device for high field strength electrotransfection of microvescicles and cells |
EP4085982A1 (en) | 2021-05-06 | 2022-11-09 | ETH Zurich | Acoustofluidic device and method for trapping particles in a fluid |
CN114196532A (en) * | 2021-11-01 | 2022-03-18 | 上海盟德生物科技有限公司 | Multi-channel cell electrotransformation instrument |
WO2024148320A1 (en) * | 2023-01-06 | 2024-07-11 | University Of Kentucky Research Foundation | 3d microfluidic transfection devices and method of transfecting target cells with a transfection agent |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0814855A4 (en) | 1995-03-10 | 2002-07-17 | Theramed Inc | Flow electroporation chamber and method |
US6773669B1 (en) | 1995-03-10 | 2004-08-10 | Maxcyte, Inc. | Flow electroporation chamber and method |
US6090617A (en) * | 1996-12-05 | 2000-07-18 | Entremed, Inc. | Flow electroporation chamber with electrodes having a crystalline metal nitride coating |
US7029916B2 (en) * | 2001-02-21 | 2006-04-18 | Maxcyte, Inc. | Apparatus and method for flow electroporation of biological samples |
SE522801C2 (en) * | 2001-03-09 | 2004-03-09 | Erysave Ab | Apparatus for separating suspended particles from an ultrasonic fluid and method for such separation |
DK1456345T3 (en) * | 2001-08-22 | 2016-09-26 | Maxcyte Inc | Apparatus and method for electroporation of biological samples |
EP2156178B1 (en) | 2007-04-02 | 2011-12-21 | Acoustic Cytometry Systems, Inc. | Methods for enhanced analysis of acoustic field focused cells and particles |
US9816086B2 (en) | 2010-07-06 | 2017-11-14 | The Ohio State University | Dose and location controlled drug/gene/particle delivery to individual cells by nanoelectroporation |
DE102014200505A1 (en) * | 2014-01-14 | 2015-07-16 | Robert Bosch Gmbh | Container and device for the digestion and / or homogenization of a fluid and method for digesting and / or homogenizing a fluid |
JP6846345B2 (en) | 2014-11-14 | 2021-03-24 | マサチューセッツ インスティテュート オブ テクノロジー | Destruction and field delivery of compounds and compositions to cells |
WO2016187535A2 (en) * | 2015-05-20 | 2016-11-24 | Zephyrus Biosciences, Inc. | System and methods for electrophoretic separation and analysis of analytes |
KR20180042422A (en) | 2015-09-04 | 2018-04-25 | 룻거스, 더 스테이트 유니버시티 오브 뉴저지 | High-efficiency, feedback-controlled electroporation microdevices for efficient molecular delivery to single cells |
US11225638B2 (en) * | 2016-04-04 | 2022-01-18 | CyteQuest, Inc. | System, device and method for electroporation of cells |
CN107988070A (en) | 2017-06-13 | 2018-05-04 | 北京呈诺医学科技有限公司 | A kind of few cells electricity turns micro-fluidic chip, electricity turns sorter and application |
CN107699485B (en) * | 2017-11-06 | 2020-08-11 | 东南大学 | Microelectrode flow control chip and parameter-adjustable single-cell electroporation device |
WO2020014538A1 (en) | 2018-07-12 | 2020-01-16 | Gpb Scientific, Llc | Microfluidic methods for the preparation of cells |
-
2019
- 2019-08-30 CN CN201980068022.2A patent/CN113166706A/en active Pending
- 2019-08-30 KR KR1020217009598A patent/KR20210089640A/en unknown
- 2019-08-30 SG SG11202102008XA patent/SG11202102008XA/en unknown
- 2019-08-30 AU AU2019331113A patent/AU2019331113A1/en active Pending
- 2019-08-30 JP JP2021510943A patent/JP2021534793A/en active Pending
- 2019-08-30 US US16/557,820 patent/US11859162B2/en active Active
- 2019-08-30 WO PCT/US2019/049210 patent/WO2020047504A2/en active Application Filing
- 2019-08-30 CA CA3111720A patent/CA3111720A1/en active Pending
- 2019-08-30 EP EP19769666.9A patent/EP3844261A2/en active Pending
-
2021
- 2021-02-28 IL IL281138A patent/IL281138A/en unknown
-
2023
- 2023-11-21 US US18/515,493 patent/US20240084236A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CA3111720A1 (en) | 2020-03-05 |
WO2020047504A3 (en) | 2020-04-09 |
AU2019331113A1 (en) | 2021-03-18 |
US11859162B2 (en) | 2024-01-02 |
US20200071727A1 (en) | 2020-03-05 |
CN113166706A (en) | 2021-07-23 |
IL281138A (en) | 2021-04-29 |
KR20210089640A (en) | 2021-07-16 |
US20240084236A1 (en) | 2024-03-14 |
WO2020047504A2 (en) | 2020-03-05 |
JP2021534793A (en) | 2021-12-16 |
EP3844261A2 (en) | 2021-07-07 |
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