SG11202004691WA - Complex for genome editing having stability and few side-effects, and nucleic acid coding same - Google Patents

Complex for genome editing having stability and few side-effects, and nucleic acid coding same

Info

Publication number
SG11202004691WA
SG11202004691WA SG11202004691WA SG11202004691WA SG11202004691WA SG 11202004691W A SG11202004691W A SG 11202004691WA SG 11202004691W A SG11202004691W A SG 11202004691WA SG 11202004691W A SG11202004691W A SG 11202004691WA SG 11202004691W A SG11202004691W A SG 11202004691WA
Authority
SG
Singapore
Prior art keywords
complex
stability
effects
nucleic acid
genome editing
Prior art date
Application number
SG11202004691WA
Inventor
Keiji Nishida
Original Assignee
Univ Kobe Nat Univ Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kobe Nat Univ Corp filed Critical Univ Kobe Nat Univ Corp
Publication of SG11202004691WA publication Critical patent/SG11202004691WA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
    • C12Y302/02027—Uracil-DNA glycosylase (3.2.2.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
SG11202004691WA 2017-11-22 2018-11-21 Complex for genome editing having stability and few side-effects, and nucleic acid coding same SG11202004691WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017225221 2017-11-22
PCT/JP2018/042915 WO2019103020A1 (en) 2017-11-22 2018-11-21 Complex for genome editing having stability and few side-effects, and nucleic acid coding same

Publications (1)

Publication Number Publication Date
SG11202004691WA true SG11202004691WA (en) 2020-06-29

Family

ID=66632001

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202004691WA SG11202004691WA (en) 2017-11-22 2018-11-21 Complex for genome editing having stability and few side-effects, and nucleic acid coding same

Country Status (9)

Country Link
US (1) US20210024906A1 (en)
EP (1) EP3715454A4 (en)
JP (2) JP7328695B2 (en)
KR (1) KR102387830B1 (en)
CN (1) CN111615557A (en)
BR (1) BR112020010036A2 (en)
CA (1) CA3082922C (en)
SG (1) SG11202004691WA (en)
WO (1) WO2019103020A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112019019673A2 (en) * 2017-03-22 2020-04-22 National University Corporation Kobe University method and agent for altering a targeted DNA site in a cell, complex, and nucleic acid
WO2022095929A1 (en) * 2020-11-06 2022-05-12 The University Of Hong Kong Transferable type i-f crispr-cas genome editing system
CN114292831B (en) * 2021-02-03 2023-04-07 山东舜丰生物科技有限公司 Novel Cas enzyme and application
CN113862274B (en) * 2021-09-29 2023-10-03 燕山大学 Oligonucleotide aptamer APT-D1 for high-specificity recognition of APE1 and preparation method and application thereof
CN116064429B (en) * 2022-08-04 2023-11-03 四川省畜牧科学研究院 Method for efficiently expressing laccase by recombinant escherichia coli
WO2024094084A1 (en) * 2022-11-01 2024-05-10 Huidagene Therapeutics Co., Ltd. Iscb polypeptides and uses thereof
CN117947054B (en) * 2024-03-25 2024-06-21 上海羽冠生物技术有限公司 Method for editing pertussis Bao Te bacteria seamless genes

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4968498B2 (en) 2002-01-23 2012-07-04 ユニバーシティ オブ ユタ リサーチ ファウンデーション Targeted chromosomal mutagenesis using zinc finger nuclease
JP2013128413A (en) 2010-03-11 2013-07-04 Kyushu Univ Method for modifying rna-binding protein using ppr motif
RU2595840C2 (en) 2011-10-04 2016-08-27 АйЭйчАй КОРПОРЕЙШН Therapeutic agent, sensitive to metal-salen complex compound, and system for regulation of intracorporeal properties for metal-salen complex compound
JP6206893B2 (en) 2014-03-05 2017-10-04 国立大学法人神戸大学 Method for modifying genomic sequence to specifically convert nucleobase of targeted DNA sequence and molecular complex used therefor
KR102630014B1 (en) * 2014-10-01 2024-01-25 더 제너럴 하스피탈 코포레이션 Methods for increasing efficiency of nuclease-induced homology-directed repair
PT3216867T (en) 2014-11-04 2020-07-16 Univ Kobe Nat Univ Corp Method for modifying genome sequence to introduce specific mutation to targeted dna sequence by base-removal reaction, and molecular complex used therein
CN104531632A (en) * 2014-11-18 2015-04-22 李云英 Rapidly-degraded Cas9-ODC422-461 fusion protein and application thereof
US20160362667A1 (en) * 2015-06-10 2016-12-15 Caribou Biosciences, Inc. CRISPR-Cas Compositions and Methods
IL293323B2 (en) * 2015-06-18 2024-01-01 Massachusetts Inst Technology Crispr enzyme mutations reducing off-target effects
JP6909475B2 (en) 2016-06-14 2021-07-28 有限会社メカノトランスフォーマ How to draw lead wires for actuators and telescopic elements
WO2018145068A1 (en) * 2017-02-06 2018-08-09 Trustees Of Boston University An integrated system for programmable dna methylation
WO2018226880A1 (en) * 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving escherichia coli

Also Published As

Publication number Publication date
BR112020010036A2 (en) 2020-10-20
JP7328695B2 (en) 2023-08-17
JP2023129709A (en) 2023-09-14
CA3082922A1 (en) 2019-05-31
CA3082922C (en) 2024-02-13
KR102387830B1 (en) 2022-04-18
EP3715454A4 (en) 2021-08-18
CN111615557A (en) 2020-09-01
KR20200091884A (en) 2020-07-31
EP3715454A1 (en) 2020-09-30
JPWO2019103020A1 (en) 2021-01-21
WO2019103020A1 (en) 2019-05-31
US20210024906A1 (en) 2021-01-28

Similar Documents

Publication Publication Date Title
IL273307A (en) Polynucleotides, compositions, and methods for genome editing
SG11202004691WA (en) Complex for genome editing having stability and few side-effects, and nucleic acid coding same
HK1254230A1 (en) Tat-induced crispr/endonuclease-based gene editing
EP3692145A4 (en) Systems, methods, and compositions for targeted nucleic acid editing
EP3684397A4 (en) Systems, methods, and compositions for targeted nucleic acid editing
EP3625340A4 (en) Genome editing system
EP3701042A4 (en) Systems, methods, and compositions for targeted nucleic acid editing
PH12018501159A1 (en) Composition and methods for immunooncology
HK1253403A1 (en) Genome editing vectors
EP3469084A4 (en) High specificity genome editing using chemically modified guide rnas
EP3303585A4 (en) Dna editing using single-stranded dna
EP3500271A4 (en) Genome editing enhancers
EP3353309A4 (en) Compositions and methods for genome editing
EP3601615A4 (en) Genomic stability profiling
GB201613135D0 (en) Genome editing
EP3487992A4 (en) Methods and compositions for modifying genomic dna
EP3224363A4 (en) Nucleic acid constructs for genome editing
EP3348636A4 (en) Method for modifying genome sequence that specifically converts nucleobase of targeted dna sequence, and molecular complex used in said method
EP3353298A4 (en) Allele selective gene editing and uses thereof
EP3630350A4 (en) Homopolymer encoded nucleic acid memory
EP3316676A4 (en) Haploid inducer line for accelerated genome editing
IL271239A (en) Nucleic acid indexing techniques
EP3427822A4 (en) Catalyst for preparing 2,5-furancarboxylic acid and method for preparing 2,5-furancarboxylic acid using catalyst
EP3186375A4 (en) Novel cas9 proteins and guiding features for dna targeting and genome editing
EP3521430A4 (en) Double-stranded nucleic acid complex having overhang