EP3224363A4 - Nucleic acid constructs for genome editing - Google Patents
Nucleic acid constructs for genome editing Download PDFInfo
- Publication number
- EP3224363A4 EP3224363A4 EP15862422.1A EP15862422A EP3224363A4 EP 3224363 A4 EP3224363 A4 EP 3224363A4 EP 15862422 A EP15862422 A EP 15862422A EP 3224363 A4 EP3224363 A4 EP 3224363A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- nucleic acid
- genome editing
- acid constructs
- constructs
- editing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000010362 genome editing Methods 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203—Virus mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462085292P | 2014-11-27 | 2014-11-27 | |
PCT/IL2015/051150 WO2016084084A1 (en) | 2014-11-27 | 2015-11-26 | Nucleic acid constructs for genome editing |
Publications (3)
Publication Number | Publication Date |
---|---|
EP3224363A1 EP3224363A1 (en) | 2017-10-04 |
EP3224363A4 true EP3224363A4 (en) | 2018-05-23 |
EP3224363B1 EP3224363B1 (en) | 2021-11-03 |
Family
ID=56073731
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP15862422.1A Active EP3224363B1 (en) | 2014-11-27 | 2015-11-26 | Nucleic acid constructs for genome editing |
Country Status (7)
Country | Link |
---|---|
US (1) | US10883111B2 (en) |
EP (1) | EP3224363B1 (en) |
JP (1) | JP2017535296A (en) |
KR (1) | KR20170081268A (en) |
CA (1) | CA2968808A1 (en) |
IL (1) | IL252556A0 (en) |
WO (1) | WO2016084084A1 (en) |
Families Citing this family (41)
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US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
LT3456831T (en) | 2013-04-16 | 2021-09-10 | Regeneron Pharmaceuticals, Inc. | Targeted modification of rat genome |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
AU2014360811B2 (en) | 2013-12-11 | 2017-05-18 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for the targeted modification of a genome |
US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
RU2020134412A (en) | 2014-11-21 | 2020-11-11 | Регенерон Фармасьютикалз, Инк. | METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION WITH THE USE OF PAIRED GUIDE RNA |
KR20170081268A (en) | 2014-11-27 | 2017-07-11 | 단지거 이노베이션즈 엘티디. | Nucleic acid constructs for genome editing |
BR112017015988B1 (en) | 2015-01-27 | 2024-02-27 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | METHODS FOR SITE-TARGETED MODIFICATION OF A TARGET FRAGMENT OF A TARGET GENE IN A WHOLE PLANT AND FOR OBTAINING A TRANSGENE-FREE MUTANT PLANT |
EP3095870A1 (en) | 2015-05-19 | 2016-11-23 | Kws Saat Se | Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom |
CA2994883A1 (en) | 2015-08-14 | 2017-02-23 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Scnces | Method for obtaining glyphosate-resistant rice by site-directed nucleotide substitution |
IL258821B (en) | 2015-10-23 | 2022-07-01 | Harvard College | Nucleobase editors and uses thereof |
KR102547316B1 (en) | 2016-08-03 | 2023-06-23 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | Adenosine nucleobase editing agents and uses thereof |
CN109804066A (en) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | Programmable CAS9- recombination enzyme fusion proteins and application thereof |
WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
US20190225974A1 (en) | 2016-09-23 | 2019-07-25 | BASF Agricultural Solutions Seed US LLC | Targeted genome optimization in plants |
KR102622411B1 (en) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | AAV delivery of nucleobase editor |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
GB2575930A (en) | 2017-03-23 | 2020-01-29 | Harvard College | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
ES2693895A1 (en) | 2017-06-12 | 2018-12-14 | Consejo Superior De Investigaciones Científicas (Csic) | BINARY VECTORS AND USES OF THEM (Machine-translation by Google Translate, not legally binding) |
US20190032067A1 (en) * | 2017-07-28 | 2019-01-31 | Organic Genomics, Inc. | Automated methods and systems for genetically enhancing genomes in computer controlled environments utilizing recurrently segmented nucleotide replications |
JP2020534795A (en) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Methods and Compositions for Evolving Base Editing Factors Using Phage-Supported Continuous Evolution (PACE) |
EP3661354A1 (en) * | 2017-07-31 | 2020-06-10 | R. J. Reynolds Tobacco Company | Methods and compositions for viral-based gene editing in plants |
EP3676376A2 (en) | 2017-08-30 | 2020-07-08 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
WO2019079347A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
WO2019143077A1 (en) | 2018-01-22 | 2019-07-25 | 경상대학교산학협력단 | Method for increasing efficiency of homologous recombination-based gene editing in plant |
KR102074744B1 (en) | 2018-09-11 | 2020-02-07 | 경상대학교산학협력단 | Virus-based replicon for plant genome editing without inserting replicon into plant genome and uses thereof |
WO2020191249A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
WO2020226176A1 (en) * | 2019-05-09 | 2020-11-12 | グランドグリーン株式会社 | Polynucleotide used for plant genome editing |
CN111304240B (en) * | 2020-03-25 | 2022-02-08 | 山东农业大学 | Method for rapidly identifying grape gene function based on tobacco transient expression system |
CN111235181B (en) * | 2020-03-25 | 2022-09-23 | 江苏科技大学 | Virus vector for efficiently screening gene editing crops without exogenous DNA (deoxyribonucleic acid), and construction method and application thereof |
MX2022014008A (en) | 2020-05-08 | 2023-02-09 | Broad Inst Inc | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence. |
JP2024514696A (en) | 2021-04-21 | 2024-04-02 | 浙江大学 | Minus-strand RNA viral vector and plant genome editing method that does not require transformation |
CN114621974B (en) * | 2022-03-04 | 2023-11-21 | 江苏科技大学 | Vector of plant single-gene or multi-gene CRISPR (clustered regularly interspaced short palindromic surface plasmon) activation technology, and construction method and application thereof |
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2015
- 2015-11-26 KR KR1020177016807A patent/KR20170081268A/en unknown
- 2015-11-26 JP JP2017547193A patent/JP2017535296A/en active Pending
- 2015-11-26 WO PCT/IL2015/051150 patent/WO2016084084A1/en active Application Filing
- 2015-11-26 US US15/531,436 patent/US10883111B2/en active Active
- 2015-11-26 EP EP15862422.1A patent/EP3224363B1/en active Active
- 2015-11-26 CA CA2968808A patent/CA2968808A1/en not_active Abandoned
-
2017
- 2017-05-28 IL IL252556A patent/IL252556A0/en unknown
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WO2009130695A2 (en) * | 2008-04-21 | 2009-10-29 | Danziger Innovation Ltd. | Plant viral expression vectors and use of same for generating genotypic variations in plant genomes |
WO2011048600A1 (en) * | 2009-10-21 | 2011-04-28 | Danziger Innovations Ltd. | Generating genotypic variations in plant genomes by gamete infection |
WO2015189693A1 (en) * | 2014-06-12 | 2015-12-17 | King Abdullah University Of Science And Technology | Targeted viral-mediated plant genome editing using crispr/cas9 |
Non-Patent Citations (3)
Title |
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See also references of WO2016084084A1 * |
SYED SHAN-E-ALI ZAIDI ET AL: "Viral Vectors for Plant Genome Engineering", FRONTIERS IN PLANT SCIENCE, vol. 8, 11 April 2017 (2017-04-11), CH, XP055465212, ISSN: 1664-462X, DOI: 10.3389/fpls.2017.00539 * |
ZAHIR ALI ET AL: "Activity and specificity of TRV-mediated gene editing in plants", PLANT SIGNALING & BEHAVIOR, vol. 10, no. 10, 3 October 2015 (2015-10-03), US, pages e1044191, XP055465491, ISSN: 1559-2316, DOI: 10.1080/15592324.2015.1044191 * |
Also Published As
Publication number | Publication date |
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EP3224363A1 (en) | 2017-10-04 |
IL252556A0 (en) | 2017-07-31 |
US20170260536A1 (en) | 2017-09-14 |
US10883111B2 (en) | 2021-01-05 |
JP2017535296A (en) | 2017-11-30 |
CA2968808A1 (en) | 2016-06-02 |
WO2016084084A1 (en) | 2016-06-02 |
KR20170081268A (en) | 2017-07-11 |
EP3224363B1 (en) | 2021-11-03 |
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