SG11202002009PA - Methods for preventing disulfide bond reduction in cell culture harvest - Google Patents

Methods for preventing disulfide bond reduction in cell culture harvest

Info

Publication number
SG11202002009PA
SG11202002009PA SG11202002009PA SG11202002009PA SG11202002009PA SG 11202002009P A SG11202002009P A SG 11202002009PA SG 11202002009P A SG11202002009P A SG 11202002009PA SG 11202002009P A SG11202002009P A SG 11202002009PA SG 11202002009P A SG11202002009P A SG 11202002009PA
Authority
SG
Singapore
Prior art keywords
methods
cell culture
disulfide bond
bond reduction
culture harvest
Prior art date
Application number
SG11202002009PA
Other languages
English (en)
Inventor
Kathryn Kwant
Original Assignee
Bayer Healthcare Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Healthcare Llc filed Critical Bayer Healthcare Llc
Publication of SG11202002009PA publication Critical patent/SG11202002009PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39591—Stabilisation, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11202002009PA 2017-10-02 2018-09-26 Methods for preventing disulfide bond reduction in cell culture harvest SG11202002009PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762566746P 2017-10-02 2017-10-02
PCT/US2018/052800 WO2019070469A1 (en) 2017-10-02 2018-09-26 METHODS FOR PREVENTING DISULFIDE LINK REDUCTION IN A CELL CULTURE HARVEST

Publications (1)

Publication Number Publication Date
SG11202002009PA true SG11202002009PA (en) 2020-04-29

Family

ID=63896645

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202002009PA SG11202002009PA (en) 2017-10-02 2018-09-26 Methods for preventing disulfide bond reduction in cell culture harvest

Country Status (15)

Country Link
US (1) US20200237911A1 (ko)
EP (2) EP4074340A1 (ko)
JP (1) JP2020535825A (ko)
KR (1) KR20200058425A (ko)
CN (1) CN111182922A (ko)
AU (1) AU2018345511A1 (ko)
BR (1) BR112020006487A2 (ko)
CA (1) CA3077752A1 (ko)
IL (1) IL273593A (ko)
MX (1) MX2020003011A (ko)
PE (1) PE20201147A1 (ko)
RU (1) RU2020115256A (ko)
SG (1) SG11202002009PA (ko)
TW (1) TW201927335A (ko)
WO (1) WO2019070469A1 (ko)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050095605A (ko) * 2003-01-09 2005-09-29 제넨테크, 인크. 폴리펩티드의 정제
KR20190140090A (ko) * 2007-07-09 2019-12-18 제넨테크, 인크. 폴리펩티드의 재조합 생산 동안의 디술피드 결합 환원의 방지
JP7170542B2 (ja) * 2016-05-10 2022-11-14 メディミューン，エルエルシー タンパク質ジスルフィド結合の還元の防止
RU2759602C2 (ru) * 2017-02-08 2021-11-16 Пфайзер Инк. Процесс крупномасштабного производства кэпированных и некэпированных цистеинов антител и их применение для конъюгации терапевтических белков

Also Published As

Publication number Publication date
RU2020115256A (ru) 2021-11-08
EP3691683A1 (en) 2020-08-12
TW201927335A (zh) 2019-07-16
CN111182922A (zh) 2020-05-19
BR112020006487A2 (pt) 2020-10-13
EP3691683B1 (en) 2022-03-16
CA3077752A1 (en) 2019-04-11
US20200237911A1 (en) 2020-07-30
KR20200058425A (ko) 2020-05-27
IL273593A (en) 2020-05-31
WO2019070469A1 (en) 2019-04-11
PE20201147A1 (es) 2020-10-26
JP2020535825A (ja) 2020-12-10
EP4074340A1 (en) 2022-10-19
RU2020115256A3 (ko) 2022-02-16
MX2020003011A (es) 2020-07-22
AU2018345511A1 (en) 2020-03-26

Similar Documents

Publication Publication Date Title
ZA201608303B (en) Methods for harvesting mammalian cell cultures
EP3534705A4 (en) COMPOSITIONS AND METHODS FOR CYCLE PRESERVATION OF CELLS
IL246847A0 (en) Systems and methods for growing and harvesting cells
EP3331582A4 (en) METHODS AND COMPOSITIONS FOR THE TREATMENT OF TRANSPLANTATION CELLS
SG11201606934SA (en) Improved reprogramming methods and cell culture platforms
GB201611982D0 (en) Cell culture
EP3384038A4 (en) METHODS AND COMPOSITIONS FOR CELL REPROGRAMMING
SG11202004935UA (en) Cell culture methods
EP3325612A4 (en) Methods and compositions for stem cell transplantation
PT3365107T (pt) Cultura celular
EP3340997A4 (en) METHODS FOR TRANSPLANTATION OF STEM CELLS
IL290590A (en) Methods for growing cells
EP3321351A4 (en) Cell-holding container and cell culture method using same
EP3318630A4 (en) METHOD FOR SORTING TISSUE CELLS
EP3630946C0 (en) CELL CULTURE METHODS
PL3287521T3 (pl) Hodowla komórkowa
IL273593A (en) Methods for preventing disulfide bond reduction in cell culture crops
EP3119926A4 (en) Multi-junction artificial photosynthetic cell with enhanced photovoltages
IL273302A (en) Methods to prevent reduction of disulfide bonds in harvesting culture cells with selenite
GB201715518D0 (en) Cell cultivation methods
GB201707301D0 (en) Sickle cell technology
SG10202010002RA (en) Cell culture
EP3191576A4 (en) Methods for quantifying particulates in cell culture